LOCUS SIT99470.1 372 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE CYSTEINE SYNTHASE A CYSK2 (O-ACETYLSERINE SULFHYDRYLASE) (O-ACETYLSERINE (THIOL)-LYASE) (CSASE) protein. ACCESSION LT708304-882 PROTEIN_ID SIT99470.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cysK2" /locus_tag="BQ2027_MB0871" /note="Mb0871, cysK2, len: 372 aa. Equivalent to Rv0848, len: 372 aa, from Mycobacterium tuberculosis strain H37Rv, (99.5% identity in 372 aa overlap). Possible cysK2, cysteine synthase A (EC 4.2.99.8), but could be also a cysteine synthase B (EC 4.2.99.8) cysM2-product, similar to many e.g. NP_109408.1|NC_002682 cysteine synthase from Mesorhizobium loti (357 aa); Q44004|CYSM_ALCEU CYSTEINE SYNTHASE from Alcaligenes eutrophus strain CH34 (Ralstonia eutropha) (339 aa), FASTA scores: opt: 511, E(): 1.7e-25, (35.0% identity in 314 aa overlap); etc. BELONGS TO THE CYSTEINE SYNTHASE/CYSTATHIONINE BETA-SYNTHASE FAMILY. COFACTOR: PYRIDOXAL PHOSPHATE. Note that previously known as cysM3. Protein product from Mb0871 detected using SWATH mass spectrometry. Mb0871 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWM7" /db_xref="InterPro:IPR001926" /db_xref="InterPro:IPR036052" /db_xref="UniProtKB/TrEMBL:A0A1R3XWM7" BEGIN 1 MRSRQTRDRY RLLPEGYQVT PGRNRHPGTM VGNTPVLWIP ELSGTSDPDR GFWAKLEGFN 61 PGGMKDRPAL YMVECARARG DIAPGAAIVE STSGTLGLGL ALAGKVYRHP VTLVTDPGLE 121 PIIARMLTAY GAGVDMGTQP HPVGGWQQAR KDRVAQLMAE YPGAWNPNQY GNPDNVGAYR 181 SLALELVAQL GRIDVLVCSV GTGGHSAGVA RVLREFNPDM RLIGVDTIGS TIFGQPASNR 241 LMRGLGSSIY PRNVDYRAFD EVHWVAPPEA VWACRSLAAT HYASGGWSVG AVALVAGWAA 301 RNLPADTTIA AVFPDGPQRY FDTIYNDAYC NEHELLGGQP PTEPDEIASP LDAVVTRWTR 361 STTVIDPTQV VS //