LOCUS       SIT99470.1               372 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            CYSTEINE SYNTHASE A CYSK2 (O-ACETYLSERINE SULFHYDRYLASE)
            (O-ACETYLSERINE (THIOL)-LYASE) (CSASE) protein.
ACCESSION   LT708304-882
PROTEIN_ID  SIT99470.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cysK2"
                     /locus_tag="BQ2027_MB0871"
                     /note="Mb0871, cysK2, len: 372 aa. Equivalent to Rv0848,
                     len: 372 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.5% identity in 372 aa overlap). Possible cysK2,
                     cysteine synthase A (EC 4.2.99.8), but could be also a
                     cysteine synthase B (EC 4.2.99.8) cysM2-product, similar
                     to many e.g. NP_109408.1|NC_002682 cysteine synthase from
                     Mesorhizobium loti (357 aa); Q44004|CYSM_ALCEU CYSTEINE
                     SYNTHASE from Alcaligenes eutrophus strain CH34 (Ralstonia
                     eutropha) (339 aa), FASTA scores: opt: 511, E(): 1.7e-25,
                     (35.0% identity in 314 aa overlap); etc. BELONGS TO THE
                     CYSTEINE SYNTHASE/CYSTATHIONINE BETA-SYNTHASE FAMILY.
                     COFACTOR: PYRIDOXAL PHOSPHATE. Note that previously known
                     as cysM3. Protein product from Mb0871 detected using SWATH
                     mass spectrometry. Mb0871 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3XWM7"
                     /db_xref="InterPro:IPR001926"
                     /db_xref="InterPro:IPR036052"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XWM7"
BEGIN
        1 MRSRQTRDRY RLLPEGYQVT PGRNRHPGTM VGNTPVLWIP ELSGTSDPDR GFWAKLEGFN
       61 PGGMKDRPAL YMVECARARG DIAPGAAIVE STSGTLGLGL ALAGKVYRHP VTLVTDPGLE
      121 PIIARMLTAY GAGVDMGTQP HPVGGWQQAR KDRVAQLMAE YPGAWNPNQY GNPDNVGAYR
      181 SLALELVAQL GRIDVLVCSV GTGGHSAGVA RVLREFNPDM RLIGVDTIGS TIFGQPASNR
      241 LMRGLGSSIY PRNVDYRAFD EVHWVAPPEA VWACRSLAAT HYASGGWSVG AVALVAGWAA
      301 RNLPADTTIA AVFPDGPQRY FDTIYNDAYC NEHELLGGQP PTEPDEIASP LDAVVTRWTR
      361 STTVIDPTQV VS
//