LOCUS       SIT99446.1               338 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ACYL-[ACYL-CARRIER PROTEIN] DESATURASE DESA1 (ACYL-[ACP]
            DESATURASE) (STEAROYL-ACP DESATURASE) (PROTEIN DES) protein.
ACCESSION   LT708304-858
PROTEIN_ID  SIT99446.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="desA1"
                     /locus_tag="BQ2027_MB0847C"
                     /standard_name="des"
                     /note="Mb0847c, desA1, len: 338 aa. Equivalent to Rv0824c,
                     len: 338 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 338 aa overlap). Probable desA1
                     (alternate gene name: des), acyl-[acyl-carrier protein]
                     desaturase (stearoyl-ACP desaturase) (EC 1.14.19.2) (see
                     first citation below), equivalent to U15182|MLU15182_32
                     acyl-[ACP] desaturase from Mycobacterium leprae (338 aa),
                     FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa
                     overlap); and highly similar in part to fragment
                     CAB96061.1|AJ250019 Steroyl-ACP-desaturase from
                     Mycobacterium avium subsp. paratuberculosis (93 aa). Also
                     similar to other fatty acid desaturases e.g. T35035
                     probable acyl-[acyl-carrier protein] desaturase from
                     Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA
                     ACYL-[ACYL-CARRIER PROTEIN] DESATURASE PRECURSOR from
                     Oryza sativa (Rice) (390 aa); etc. Also highly similar to
                     desA2|Rv1094 from Mycobacterium tuberculosis (275 aa).
                     Contains PS00225 Crystallins beta and gamma 'Greek key'
                     motif signature. BELONGS TO THE FATTY ACID DESATURASE
                     FAMILY. COFACTOR: FERREDOXIN, FERREDOXIN NADPH REDUCTASE,
                     AND NADPH. Protein product from Mb0847c detected using
                     shotgun mass spectrometry and SWATH mass spectrometry.
                     Mb0847c found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XWJ1"
                     /db_xref="InterPro:IPR005067"
                     /db_xref="InterPro:IPR009078"
                     /db_xref="InterPro:IPR012348"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XWJ1"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSAKLTDLQL LHELEPVVEK YLNRHLSMHK PWNPHDYIPW SDGKNYYALG GQDWDPDQSK
       61 LSDVAQVAMV QNLVTEDNLP SYHREIAMNM GMDGAWGQWV NRWTAEENRH GIALRDYLVV
      121 TRSVDPVELE KLRLEVVNRG FSPGQNHQGH YFAESLTDSV LYVSFQELAT RISHRNTGKA
      181 CNDPVADQLM AKISADENLH MIFYRDVSEA AFDLVPNQAM KSLHLILSHF QMPGFQVPEF
      241 RRKAVVIAVG GVYDPRIHLD EVVMPVLKKW RIFEREDFTG EGAKLRDELA LVIKDLELAC
      301 DKFEVSKQRQ LDREARTGKK VSAHELHKTA GKLAMSRR
//