LOCUS SIT99446.1 338 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ACYL-[ACYL-CARRIER PROTEIN] DESATURASE DESA1 (ACYL-[ACP] DESATURASE) (STEAROYL-ACP DESATURASE) (PROTEIN DES) protein. ACCESSION LT708304-858 PROTEIN_ID SIT99446.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="desA1" /locus_tag="BQ2027_MB0847C" /standard_name="des" /note="Mb0847c, desA1, len: 338 aa. Equivalent to Rv0824c, len: 338 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 338 aa overlap). Probable desA1 (alternate gene name: des), acyl-[acyl-carrier protein] desaturase (stearoyl-ACP desaturase) (EC 1.14.19.2) (see first citation below), equivalent to U15182|MLU15182_32 acyl-[ACP] desaturase from Mycobacterium leprae (338 aa), FASTA scores: opt: 1880, E(): 0, (79.9% identity in 338 aa overlap); and highly similar in part to fragment CAB96061.1|AJ250019 Steroyl-ACP-desaturase from Mycobacterium avium subsp. paratuberculosis (93 aa). Also similar to other fatty acid desaturases e.g. T35035 probable acyl-[acyl-carrier protein] desaturase from Streptomyces coelicolor (328 aa); Q40731|STAD_ORYSA ACYL-[ACYL-CARRIER PROTEIN] DESATURASE PRECURSOR from Oryza sativa (Rice) (390 aa); etc. Also highly similar to desA2|Rv1094 from Mycobacterium tuberculosis (275 aa). Contains PS00225 Crystallins beta and gamma 'Greek key' motif signature. BELONGS TO THE FATTY ACID DESATURASE FAMILY. COFACTOR: FERREDOXIN, FERREDOXIN NADPH REDUCTASE, AND NADPH. Protein product from Mb0847c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0847c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWJ1" /db_xref="InterPro:IPR005067" /db_xref="InterPro:IPR009078" /db_xref="InterPro:IPR012348" /db_xref="UniProtKB/TrEMBL:A0A1R3XWJ1" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSAKLTDLQL LHELEPVVEK YLNRHLSMHK PWNPHDYIPW SDGKNYYALG GQDWDPDQSK 61 LSDVAQVAMV QNLVTEDNLP SYHREIAMNM GMDGAWGQWV NRWTAEENRH GIALRDYLVV 121 TRSVDPVELE KLRLEVVNRG FSPGQNHQGH YFAESLTDSV LYVSFQELAT RISHRNTGKA 181 CNDPVADQLM AKISADENLH MIFYRDVSEA AFDLVPNQAM KSLHLILSHF QMPGFQVPEF 241 RRKAVVIAVG GVYDPRIHLD EVVMPVLKKW RIFEREDFTG EGAKLRDELA LVIKDLELAC 301 DKFEVSKQRQ LDREARTGKK VSAHELHKTA GKLAMSRR //