LOCUS SIT99445.1 389 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN protein. ACCESSION LT708304-857 PROTEIN_ID SIT99445.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0846C" /note="Mb0846c, -, len: 389 aa. Equivalent to Rv0823c, len: 389 aa, from Mycobacterium tuberculosis strain H37Rv, (99.5% identity in 389 aa overlap). Possible transcriptional regulator (resembles nitrogen regulation protein), equivalent (but longer 24 aa in N-terminus) to MLU15182_31|U15182|NtrB NtrB protein from Mycobacterium leprae (384 aa), FASTA scores: opt: 2070, E(): 0, (82.3% identity in 384 aa overlap) (see citation below). Also highly similar to CAB63312.1|AL133471|SCC82.03c hypothetical protein from Streptomyces coelicolor (406 aa); and to many transcriptional regulators members of UPF0034 FAMILY (NIFR3/SMM1) e.g. D26185|BAC180K_143 protein similar to transcriptional regulator (nitrogen regulation protein) from Bacillus subtilis (333 aa), FASTA scores: opt: 609, E(): 1.4e-32, (38.3% identity in 326 aa overlap); NP_349795.1|NC_003030 NifR3 family enzyme from Clostridium acetobutylicum (321 aa); etc. Contains PS01136 Uncharacterized protein family UPF0034 signature. Protein product from Mb0846c detected using SWATH mass spectrometry. Mb0846c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWX6" /db_xref="InterPro:IPR001269" /db_xref="InterPro:IPR004652" /db_xref="InterPro:IPR013785" /db_xref="InterPro:IPR018517" /db_xref="InterPro:IPR024036" /db_xref="InterPro:IPR035587" /db_xref="UniProtKB/TrEMBL:A0A1R3XWX6" BEGIN 1 MSRRRAIQPS PALRIGPIEL ASPVVLAPMA GVTNVAFRAL CRQLEQSKVG TVSGLYVCEM 61 VTARALIERH PVTMHMTTFS ADESPRSLQL YTVDPDTTYA AARMIAGEGL ADHIDMNFGC 121 PVPKVTKRGC GAALPFKRRL FGQIVAAAVR ATEGTDIPVT VKFRIGIDDA HHTHLDAGRI 181 AEAEGAAAVA LHARTAAQRY SGTADWEQIA RLKQHVRTIP VLGNGDIYDA GDALAMMSTT 241 GCDGVVIGRG CLGRPWLFAE LSAAFTGSPA PTPPTLGEVA DIIRRHGTLL AAHFGEDKGM 301 RDIRKHIAWY LHGFPAGSAL RRALAMVKTL DELDCLLDRL DGTVPFPDSA TGARGRQGSP 361 ARVALPDGWL TDPDDCRVPE GADAMGSGG //