LOCUS SIT99431.1 364 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE PURM (AIRS) (PHOSPHORIBOSYL-AMINOIMIDAZOLE SYNTHETASE) (AIR SYNTHASE) protein. ACCESSION LT708304-843 PROTEIN_ID SIT99431.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="purM" /locus_tag="BQ2027_MB0832" /note="Mb0832, purM, len: 364 aa. Equivalent to Rv0809, len: 364 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 364 aa overlap). Probable purM, 5'-phosphoribosyl-5-aminoimidazole synthetase (EC 6.3.3.1), equivalent to NP_302446.1|NC_002677 5'-phosphoribosyl-5-aminoimidazole synthase from Mycobacterium leprae (364 aa). Also highly similar to many e.g. P12043|PUR5_BACSU PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE from Bacillus subtilis (346 aa), FASTA scores: opt: 1023, E(): 0, (46.5% identity in 331 aa overlap); U68765|STU68765_2 from Salmonella typhimurium (345 aa), FASTA scores: opt: 1014, E():0, (47.6% identity in 330 aa overlap); etc. Protein product from Mb0832 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0832 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWH4" /db_xref="InterPro:IPR004733" /db_xref="InterPro:IPR010918" /db_xref="InterPro:IPR016188" /db_xref="InterPro:IPR036676" /db_xref="InterPro:IPR036921" /db_xref="UniProtKB/TrEMBL:A0A1R3XWH4" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTDLAKGPGK DPGSRGITYA SAGVDIEAGD RAIDLFKPLA SKATRPEVRG GLGGFAGLFT 61 LRGDYREPVL AASSDGVGTK LAIAQAMDKH DTVGLDLVAM VVDDLVVCGA EPLFLLDYIA 121 VGRIVPERLS AIVAGIADGC MRAGCALLGG ETAEHPGLIE PDHYDISATG VGVVEADNVL 181 GPDRVKPGDV IIAMGSSGLH SNGYSLVRKV LLEIDRMNLA GHVEEFGRTL GEELLEPTRI 241 YAKDCLALAA ETRVRTFCHV TGGGLAGNLQ RVIPHGLIAE VDRGTWTPAP VFTMIAQRGR 301 VRRTEMEKTF NMGVGMIAVV APEDTTRALA VLTARHLDCW VLGTVCKGGK QGPRAKLVGQ 361 HPRF //