LOCUS SIT99428.1 532 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE UDP-GLUCOSE-4-EPIMERASE CPSY (GALACTOWALDENASE) (UDP-GALACTOSE- 4-EPIMERASE) (URIDINE DIPHOSPHATE GALACTOSE-4-EPIMERASE) (URIDINE DIPHOSPHO-GALACTOSE-4-EPIMERASE) protein. ACCESSION LT708304-840 PROTEIN_ID SIT99428.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cpsY" /locus_tag="BQ2027_MB0829C" /note="Mb0829c, cpsY, len: 532 aa. Equivalent to Rv0806c, len: 532 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 532 aa overlap). Possible cpsY, UDP-glucose-4-epimerase (EC 5.1.3.2), equivalent to Q50025|CPSY probable UDP-glucose-4-epimerase from Mycobacterium leprae (542 aa), FASTA scores: opt: 2964, E(): 0, (82.3% identity in 530 aa overlap). Also similar to AAC38286.1|AF019760|SACB CpsY homolog (involved in meningococcal capsule biosynthesis) from Neisseria meningitidis serogroup A (545 aa); Q51151 CAPSULE GENE COMPLEX UPD-GLUCOSE-4-EPIMERASE (GALE) from Neisseria meningitidis (373 aa), FASTA scores: opt: 496, E(): 9.5e-27, (29.3% identity in 358 aa overlap); C-terminus of CAB75373.1|AL139298 putative transferase from Streptomyces coelicolor (942 aa); and many hypothetical proteins from Streptomyces coelicolor. SEEMS TO BELONG TO THE SUGAR EPIMERASE FAMILY. Protein product from Mb0829c detected using SWATH mass spectrometry. Mb0829c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7U184" /db_xref="InterPro:IPR021520" /db_xref="InterPro:IPR031356" /db_xref="InterPro:IPR031357" /db_xref="InterPro:IPR031358" /db_xref="UniProtKB/Swiss-Prot:Q7U184" BEGIN 1 MPKISSRDGG RPAQRTVNPI IVTRRGKIAR LESGLTPQEA QIEDLVFLRK VLNRADIPYL 61 LIRNHKNRPV LAINIELRAG LERALAAACA TEPMYAKTID EPGLSPVLVA TDGLSQLVDP 121 RVVRLYRRRI APGGFRYGPA FGVELQFWVY EETVIRCPVE NSLSRKVLPR NEITPTNVKL 181 YGYKWPTLDG MFAPHASDVV FDIDMVFSWV DGSDPEFRAR RMAQMSQYVV GEGDDAEARI 241 RQIDELKYAL RSVNMFAPWI RRIFIATDST PPPWLAEHPK ITIVRAEDHF SDRSALPTYN 301 SHAVESQLHH IPGLSEHFLY SNDDMFFGRP LKASMFFSPG GVTRFIEAKT RIGLGANNPA 361 RSGFENAARV NRQLLFDRFG QVITRHLEHT AVPLRKSVLI EMEREFPEEF ARTAASPFRS 421 DTDISVTNSF YHYYALMTGR AVPQEKAKVL YVDTTSYAGL RLLPKLRKHR GYDFFCLNDG 481 SFPEVPAAQR AERVVSFLER YFPIPAPWEK IAADVSRRDF AVPRTSAPSE GA //