LOCUS SIT99422.1 433 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE AMINOPEPTIDASE PEPC protein. ACCESSION LT708304-834 PROTEIN_ID SIT99422.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pepC" /locus_tag="BQ2027_MB0823" /note="Mb0823, pepC, len: 433 aa. Equivalent to Rv0800, len: 433 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 433 aa overlap). Probable pepC, aminopeptidase I (EC 3.4.11.-), highly similar (but shorter 17 aa) to Q50022|PEPX AMINOPEPTIDASE from Mycobacterium leprae (443 aa), FASTA scores: opt: 2237, E(): 0, (78.3% identity in 433 aa overlap). Also highly similar to others from Eukaryotes and bacteria, e.g. T36482 probable aminopeptidase from Streptomyces coelicolor (432 aa), P14904|AMPL_YEAST vacuolar aminopeptidase I precursor from Saccharomyces cerevisiae (514 aa), FASTA scores: opt: 425, E(): 4.8e-21, (31.0% identity in 445 aa overlap); etc. Also similar to hypothetical proteins e.g. P38821|YHR3_YEAST hypothetical 54.2 kd protein from Saccharomyces cerevisiae (490 aa), FASTA scores: opt: 429, E(): 2.5e-21, (34.8% identity in 443 aa overlap); etc. Protein product from Mb0823 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0823 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P59951" /db_xref="InterPro:IPR001948" /db_xref="InterPro:IPR022984" /db_xref="InterPro:IPR023358" /db_xref="UniProtKB/Swiss-Prot:P59951" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MAATAHGLCE FIDASPSPFH VCATVAGRLL GAGYRELREA DRWPDKPGRY FTVRAGSLVA 61 WNAEQSGHTQ VPFRIVGAHT DSPNLRVKQH PDRLVAGWHV VALQPYGGVW LHSWLDRDLG 121 ISGRLSVRDG TGVSHRLVRI DDPILRVPQL AIHLAEDRKS LTLDPQRHIN AVWGVGERVE 181 SFVGYVAQRA GVAAADVLAA DLMTHDLTPS ALIGASVNGT ASLLSAPRLD NQASCYAGME 241 ALLAVDVDSA SSGFVPVLAI FDHEEVGSAS GHGAQSDLLS SVLERIVLAA GGTREDFLRR 301 LTTSMLASAD MAHATHPNYP DRHEPSHPIE VNAGPVLKVH PNLRYATDGR TAAAFALACQ 361 RAGVPMQRYE HRADLPCGST IGPLAAARTG IPTVDVGAAQ LAMHSARELM GAHDVAAYSA 421 ALQAFLSAEL SEA //