LOCUS       SIT99411.1               224 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE I PURG (FGAM
            SYNTHASE I) protein.
ACCESSION   LT708304-823
PROTEIN_ID  SIT99411.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="purQ"
                     /locus_tag="BQ2027_MB0812"
                     /note="Mb0812, purQ, len: 224 aa. Equivalent to Rv0788,
                     len: 224 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 224 aa overlap). Probable purQ,
                     phosphoribosylformylglycinamidine synthase I (EC 6.3.5.3),
                     equivalent to MLCB5_24|Z95151|O05756|PURQ
                     PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE I from
                     Mycobacterium leprae (224 aa), FASTA scores: opt: 1341,
                     E(): 0, (88.7% identity in 222 aa overlap). Also highly
                     similar to others e.g. P12041|PURQ_BACSU
                     PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE I from Bacillus
                     subtilis (227 aa), FASTA scores: opt: 691, E(): 8.6e-39,
                     (47.7% identity in 214 aa overlap); etc. Contains PS00442
                     Glutamine amidotransferases class-I active site. BELONGS
                     TO TYPE-1 GLUTAMINE AMIDOTRANSFERASES. Protein product
                     from Mb0812 detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb0812 found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P65903"
                     /db_xref="InterPro:IPR010075"
                     /db_xref="InterPro:IPR017926"
                     /db_xref="InterPro:IPR029062"
                     /db_xref="UniProtKB/Swiss-Prot:P65903"
BEGIN
        1 MTARIGVVTF PGTLDDVDAA RAARQVGAEV VSLWHADADL KGVDAVVVPG GFSYGDYLRA
       61 GAIARFAPVM DEVVAAADRG MPVLGICNGF QVLCEAGLLP GALTRNVGLH FICRDVWLRV
      121 ASTSTAWTSR FEPDADLLVP LKSGEGRYVA PEKVLDELEG EGRVVFRYHD NVNGSLRDIA
      181 GICSANGRVV GLMPHPEHAI EALTGPSDDG LGLFYSALDA VLTG
//