LOCUS SIT99411.1 224 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE I PURG (FGAM SYNTHASE I) protein. ACCESSION LT708304-823 PROTEIN_ID SIT99411.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="purQ" /locus_tag="BQ2027_MB0812" /note="Mb0812, purQ, len: 224 aa. Equivalent to Rv0788, len: 224 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 224 aa overlap). Probable purQ, phosphoribosylformylglycinamidine synthase I (EC 6.3.5.3), equivalent to MLCB5_24|Z95151|O05756|PURQ PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE I from Mycobacterium leprae (224 aa), FASTA scores: opt: 1341, E(): 0, (88.7% identity in 222 aa overlap). Also highly similar to others e.g. P12041|PURQ_BACSU PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE I from Bacillus subtilis (227 aa), FASTA scores: opt: 691, E(): 8.6e-39, (47.7% identity in 214 aa overlap); etc. Contains PS00442 Glutamine amidotransferases class-I active site. BELONGS TO TYPE-1 GLUTAMINE AMIDOTRANSFERASES. Protein product from Mb0812 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0812 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P65903" /db_xref="InterPro:IPR010075" /db_xref="InterPro:IPR017926" /db_xref="InterPro:IPR029062" /db_xref="UniProtKB/Swiss-Prot:P65903" BEGIN 1 MTARIGVVTF PGTLDDVDAA RAARQVGAEV VSLWHADADL KGVDAVVVPG GFSYGDYLRA 61 GAIARFAPVM DEVVAAADRG MPVLGICNGF QVLCEAGLLP GALTRNVGLH FICRDVWLRV 121 ASTSTAWTSR FEPDADLLVP LKSGEGRYVA PEKVLDELEG EGRVVFRYHD NVNGSLRDIA 181 GICSANGRVV GLMPHPEHAI EALTGPSDDG LGLFYSALDA VLTG //