LOCUS SIT99403.1 719 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable protease ii ptrbb [second part] (oligopeptidase b) protein. ACCESSION LT708304-815 PROTEIN_ID SIT99403.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ptrB" /locus_tag="BQ2027_MB0804" /note="Mb0804, ptrB, len: 719 aa. Equivalent to Rv0781 and Rv0782, len: 236 aa and 552 aa, from Mycobacterium tuberculosis strain H37Rv, (97.6% identity in 206 aa overlap and 100.0% identity in 517 aa overlap). Probable ptrB, protease II (EC 3.4.21.83), equivalent to NP_302455.1|NC_002677 protease II from Mycobacterium leprae (724 aa). Also highly similar to C-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 204, E(): 7.4e-07, (29.6% identity in 230 aa overlap); etc. Also highly similar to N-termini of many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II from Escherichia coli strains K12 and HB101 (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to be a frameshifted homologues of protease II, but we can find no error in the cosmid sequence to account for this. BELONGS TO PEPTIDASE FAMILY S9A; ALSO KNOWN AS THE PROLYL OLIGOPEPTIDASE FAMILY. Note that previously known as ptrBb. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, ptrB is split into 2 genes, ptrBa and ptrBb, due to a frameshift. In Mycobacterium bovis, a 2 bp insertion (*-gc) leads to a single product. Protein product from Mb0804 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0804 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXD9" /db_xref="InterPro:IPR001375" /db_xref="InterPro:IPR002470" /db_xref="InterPro:IPR023302" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/TrEMBL:A0A1R3XXD9" BEGIN 1 MMHRTALPSP PVAKRVQTRR EHHGDVFVDP YEWLRDKDSP EVIAYLEAEN DYTERTTAHL 61 EPLRQKIFHE IKARTKETDL SVPTRRGNWW YYARTFEGKQ YGVHCRCPVT DPDDWNPPEF 121 DERTEIPGEQ LLLDENVEAD GHDFFALGAA SVSLDDNLLA YSVDVVGDER YTLRFKDLRT 181 GEQYPDEIAG IGAGVTWAAD NRTVYYTTVD AAWRPDTVWR YRLGSGESSE RVYHEADDRF 241 WLAVGRTRSN AYLLIAAGSS ITSEVRYAHA ADPTAQFSVV LPRRDGVEYS VEHAVIAGQD 301 RFLILHNDGA VNFTLVEAPV EDPARQRTLI AHRDDVRLDA VDALAGHLVV SYRREALPRV 361 QLWPIGPDGN YGEPEEISFD SELMSAGLGP NPNWDSPKLR VGAGSFVTPV RIYDIDLVTG 421 ERTLLKEQPV LGGYRREDYV ERRDWAYGDD GTRIPVSIVH RADIEFPAPA LIYGYGAYEI 481 CEDPRFSIAR LSLLDRGMVF VVAHVRGGGE MGRLWYENGK LLDKKNTFTD FIAVARHLVD 541 TGLTSQQQLV ALGGSAGGLL MGAVANMAPD LFAGILAQVP FVDPLTTILD PSLPLTVTEW 601 DEWGNPLNDS DVYAYVKSYS PYENVTAQKY PAILAMTSLN DTRVYYVEPA KWVAALRHAK 661 TDGNSVLLKT QMHAGHGGIS GRYERWKETA FQYGWLLATA DSDRYGGGQG NDLDGAAPA //