LOCUS       SIT99403.1               719 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 probable
            protease ii ptrbb [second part] (oligopeptidase b) protein.
ACCESSION   LT708304-815
PROTEIN_ID  SIT99403.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ptrB"
                     /locus_tag="BQ2027_MB0804"
                     /note="Mb0804, ptrB, len: 719 aa. Equivalent to Rv0781 and
                     Rv0782, len: 236 aa and 552 aa, from Mycobacterium
                     tuberculosis strain H37Rv, (97.6% identity in 206 aa
                     overlap and 100.0% identity in 517 aa overlap). Probable
                     ptrB, protease II (EC 3.4.21.83), equivalent to
                     NP_302455.1|NC_002677 protease II from Mycobacterium
                     leprae (724 aa). Also highly similar to C-termini of many
                     proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845 protease II
                     from Escherichia coli strains K12 and HB101 (707 aa),
                     FASTA scores: opt: 204, E(): 7.4e-07, (29.6% identity in
                     230 aa overlap); etc. Also highly similar to N-termini of
                     many proteases II e.g. P24555|PTRB_ECOLI|TLP|B1845
                     protease II from Escherichia coli strains K12 and HB101
                     (707 aa), FASTA scores: opt: 1251, E(): 0, (42.7% identity
                     in 489 aa overlap); etc. ORFs Rv0782 and Rv0781 appear to
                     be a frameshifted homologues of protease II, but we can
                     find no error in the cosmid sequence to account for this.
                     BELONGS TO PEPTIDASE FAMILY S9A; ALSO KNOWN AS THE PROLYL
                     OLIGOPEPTIDASE FAMILY. Note that previously known as
                     ptrBb. REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, ptrB is split into 2 genes,
                     ptrBa and ptrBb, due to a frameshift. In Mycobacterium
                     bovis, a 2 bp insertion (*-gc) leads to a single product.
                     Protein product from Mb0804 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0804 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XXD9"
                     /db_xref="InterPro:IPR001375"
                     /db_xref="InterPro:IPR002470"
                     /db_xref="InterPro:IPR023302"
                     /db_xref="InterPro:IPR029058"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XXD9"
BEGIN
        1 MMHRTALPSP PVAKRVQTRR EHHGDVFVDP YEWLRDKDSP EVIAYLEAEN DYTERTTAHL
       61 EPLRQKIFHE IKARTKETDL SVPTRRGNWW YYARTFEGKQ YGVHCRCPVT DPDDWNPPEF
      121 DERTEIPGEQ LLLDENVEAD GHDFFALGAA SVSLDDNLLA YSVDVVGDER YTLRFKDLRT
      181 GEQYPDEIAG IGAGVTWAAD NRTVYYTTVD AAWRPDTVWR YRLGSGESSE RVYHEADDRF
      241 WLAVGRTRSN AYLLIAAGSS ITSEVRYAHA ADPTAQFSVV LPRRDGVEYS VEHAVIAGQD
      301 RFLILHNDGA VNFTLVEAPV EDPARQRTLI AHRDDVRLDA VDALAGHLVV SYRREALPRV
      361 QLWPIGPDGN YGEPEEISFD SELMSAGLGP NPNWDSPKLR VGAGSFVTPV RIYDIDLVTG
      421 ERTLLKEQPV LGGYRREDYV ERRDWAYGDD GTRIPVSIVH RADIEFPAPA LIYGYGAYEI
      481 CEDPRFSIAR LSLLDRGMVF VVAHVRGGGE MGRLWYENGK LLDKKNTFTD FIAVARHLVD
      541 TGLTSQQQLV ALGGSAGGLL MGAVANMAPD LFAGILAQVP FVDPLTTILD PSLPLTVTEW
      601 DEWGNPLNDS DVYAYVKSYS PYENVTAQKY PAILAMTSLN DTRVYYVEPA KWVAALRHAK
      661 TDGNSVLLKT QMHAGHGGIS GRYERWKETA FQYGWLLATA DSDRYGGGQG NDLDGAAPA
//