LOCUS SIT99399.1 472 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ADENYLOSUCCINATE LYASE PURB (ADENYLOSUCCINASE) (ASL) ( ASASE) protein. ACCESSION LT708304-811 PROTEIN_ID SIT99399.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="purB" /locus_tag="BQ2027_MB0800" /note="Mb0800, purB, len: 472 aa. Equivalent to Rv0777, len: 472 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 472 aa overlap). Probable purB, adenylosuccinate lyase (EC 4.3.2.2), equivalent (but shorter 15 aa) to MLCB5.13|Z95151|g2076607|PURB ADENYLOSUCCINATE LYASE from Mycobacterium leprae (487 aa), FASTA scores: opt: 2640, E(): 0, (86.7% identity in 472 aa overlap). More similar to eukaryotic adenylosuccinate lyases than to prokaryotic adenylosuccinate lyases e.g. P54822|PUR8_MOUSE ADENYLOSUCCINATE LYASE from Mus musculus (484 aa), FASTA scores: opt: 762, E(): 0, (32.4% identity in 445 aa overlap); CAB99134.1|AL390188 putative adenylosuccino lyase (fragment) from Streptomyces coelicolor (362 aa); etc. Contains PS00163 Fumarate lyases signature. BELONGS TO THE LYASE 1 FAMILY, ADENYLOSSUCINATE LYASE SUBFAMILY. Protein product from Mb0800 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0800 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWE6" /db_xref="InterPro:IPR000362" /db_xref="InterPro:IPR004769" /db_xref="InterPro:IPR008948" /db_xref="InterPro:IPR019468" /db_xref="InterPro:IPR020557" /db_xref="InterPro:IPR022761" /db_xref="UniProtKB/TrEMBL:A0A1R3XWE6" BEGIN 1 MSIPNVLATR YASAEMVAIW SPEAKVVSER RLWLAVLRAQ AELGVAVADS VLADYERVVD 61 DVDLASISAR ERVLRHDVKA RIEEFNALAG HEHVHKGMTS RDLTENVEQL QIRRSLEVIF 121 AHGVAAVARL AERAVSYRDL IMAGRSHNVA AQATTLGKRF ASAAQEMMIA LRRLRELIDR 181 YPLRGIKGPM GTGQDMLDLL GGDRAALADL ERRVADFLGF ATVFNSVGQV YPRSLDHDVV 241 SALVQLGAGP SSLAHTIRLM AGHELATEGF APGQVGSSAM PHKMNTRSCE RVNGLQVVLR 301 GYASMVAELA GAQWNEGDVF CSVVRRVALP DSFFAVDGQI ETFLTVLDEF GAYPAVIGRE 361 LDRYLPFLAT TKVLMAAVRA GMGRESAHRL ISEHAVATAL AMREHGAEPD LLDRLAADPR 421 LPLGRDALEA ALADKKAFAG AAGDQVDDVV AMVDALVSRY PDAAKYTPGA IL //