LOCUS       SIT99399.1               472 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ADENYLOSUCCINATE LYASE PURB (ADENYLOSUCCINASE) (ASL) (
            ASASE) protein.
ACCESSION   LT708304-811
PROTEIN_ID  SIT99399.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="purB"
                     /locus_tag="BQ2027_MB0800"
                     /note="Mb0800, purB, len: 472 aa. Equivalent to Rv0777,
                     len: 472 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 472 aa overlap). Probable purB,
                     adenylosuccinate lyase (EC 4.3.2.2), equivalent (but
                     shorter 15 aa) to MLCB5.13|Z95151|g2076607|PURB
                     ADENYLOSUCCINATE LYASE from Mycobacterium leprae (487 aa),
                     FASTA scores: opt: 2640, E(): 0, (86.7% identity in 472 aa
                     overlap). More similar to eukaryotic adenylosuccinate
                     lyases than to prokaryotic adenylosuccinate lyases e.g.
                     P54822|PUR8_MOUSE ADENYLOSUCCINATE LYASE from Mus musculus
                     (484 aa), FASTA scores: opt: 762, E(): 0, (32.4% identity
                     in 445 aa overlap); CAB99134.1|AL390188 putative
                     adenylosuccino lyase (fragment) from Streptomyces
                     coelicolor (362 aa); etc. Contains PS00163 Fumarate lyases
                     signature. BELONGS TO THE LYASE 1 FAMILY, ADENYLOSSUCINATE
                     LYASE SUBFAMILY. Protein product from Mb0800 detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb0800 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3XWE6"
                     /db_xref="InterPro:IPR000362"
                     /db_xref="InterPro:IPR004769"
                     /db_xref="InterPro:IPR008948"
                     /db_xref="InterPro:IPR019468"
                     /db_xref="InterPro:IPR020557"
                     /db_xref="InterPro:IPR022761"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XWE6"
BEGIN
        1 MSIPNVLATR YASAEMVAIW SPEAKVVSER RLWLAVLRAQ AELGVAVADS VLADYERVVD
       61 DVDLASISAR ERVLRHDVKA RIEEFNALAG HEHVHKGMTS RDLTENVEQL QIRRSLEVIF
      121 AHGVAAVARL AERAVSYRDL IMAGRSHNVA AQATTLGKRF ASAAQEMMIA LRRLRELIDR
      181 YPLRGIKGPM GTGQDMLDLL GGDRAALADL ERRVADFLGF ATVFNSVGQV YPRSLDHDVV
      241 SALVQLGAGP SSLAHTIRLM AGHELATEGF APGQVGSSAM PHKMNTRSCE RVNGLQVVLR
      301 GYASMVAELA GAQWNEGDVF CSVVRRVALP DSFFAVDGQI ETFLTVLDEF GAYPAVIGRE
      361 LDRYLPFLAT TKVLMAAVRA GMGRESAHRL ISEHAVATAL AMREHGAEPD LLDRLAADPR
      421 LPLGRDALEA ALADKKAFAG AAGDQVDDVV AMVDALVSRY PDAAKYTPGA IL
//