LOCUS SIT99396.1 303 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED EXPORTED PROTEIN protein. ACCESSION LT708304-808 PROTEIN_ID SIT99396.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0797C" /note="Mb0797c, -, len: 303 aa. Equivalent to Rv0774c, len: 303 aa, from Mycobacterium tuberculosis strain H37Rv, (99.3% identity in 303 aa overlap). Possible conserved exported protein with hydrophobic region near N-terminus, highly similar, except in N-terminus, to Rv0519c|Z97831|MTY20G10.09c|O33364 HYPOTHETICAL PROTEIN from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 1092, E(): 0, (57.9% identity in 299 aa overlap). Contains PS00061 Short-chain alcohol dehydrogenase family signature, and PS00120 Lipases, serine active site. So could be a lipase (EC 3.1.-.-). Protein product from Mb0797c detected using SWATH mass spectrometry. Mb0797c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="InterPro:IPR000801" /db_xref="InterPro:IPR006311" /db_xref="InterPro:IPR029058" /db_xref="UniProtKB/TrEMBL:A0A1R3XWD7" BEGIN 1 MMARMPELSR RAVLGLGAGT VLGATSAYAI DMLLQPRTSH AAPAAAIGTN VPLAPTPALD 61 PAPPAQAAPT MSTGSFVSAA RAGKMTNWAI ARPPGQTQAL RPVIALHGLG GSASAVMDGG 121 VEQGLAQAVN AGLPPFAVVS VAGGSSYWHQ RASGEDAGAM VLNELIPLLD TQRLDTSRVA 181 FLGWSMGGYG ALLLGSRLGP ARTAAICAVS PALWLSAGAV APGSFDGPDD WSANSVFGLP 241 ALGSIPIRVD CGNSDPFYAA TKQFVAQLPH PPAGGFSPGG HNGGFWSAQL PAELTWFAPL 301 LTG //