LOCUS SIT99393.1 144 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE 4-CARBOXYMUCONOLACTONE DECARBOXYLASE (CMD) protein. ACCESSION LT708304-805 PROTEIN_ID SIT99393.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0794" /note="Mb0794, -, len: 144 aa. Equivalent to Rv0771, len: 144 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 144 aa overlap). Possible 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), showing similarity with other carboxymuconolactone decarboxylases e.g. AAD39557.1|AF031417 PcaC-like protein from Pseudomonas putida (130 aa); P20370|DC4C_ACICA 4-CARBOXYMUCONOLACTONE DECARBOXYLASE (CMD) from Acinetobacter sp. ADP1 (134 aa), FASTA scores: opt: 174, E(): 0.00075, (31.4% identity in 121 aa overlap); C-terminus of NP_421214.1|NC_002696 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase from Caulobacter crescentus (393 aa); C-terminus of T47115 probable 4-carboxymuconolactone decarboxylase / 3-oxoadipate enol-lactone hydrolase from Streptomyces sp (373 aa); NP_407104.1|NC_003143 putative gamma carboxymuconolactone decarboxylase from Yersinia pestis (131 aa); etc. Protein product from Mb0794 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0794 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XXC9" /db_xref="InterPro:IPR003779" /db_xref="InterPro:IPR029032" /db_xref="UniProtKB/TrEMBL:A0A1R3XXC9" BEGIN 1 MMDELRRTGL DKMNEVYAWD MPDMPGEFFA LTVDHLFGRI WTRPGLSMRD RRMAVIAVLT 61 AQGQSDLLEV QVNAVLHNDE LTIDELRELA VFITHYVGFP LGSRLNSAIE RVAAKRKQAA 121 ENGSLPDTKA NVAEVLAKES GKSS //