LOCUS SIT99392.1 295 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE DEHYDROGENASE/REDUCTASE protein. ACCESSION LT708304-804 PROTEIN_ID SIT99392.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0793" /note="Mb0793, -, len: 295 aa. Equivalent to Rv0770, len: 295 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 295 aa overlap). Probable dehydrogenase/reductase, 3-hydroxyisobutyrate dehydrogenase family (EC 1.1.1.-), possibly 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) or 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), similar to others e.g. P23523|GARR_ECOLI 2-HYDROXY-3-OXOPROPIONATE REDUCTASE (TARTRONATE SEMIALDEHYDE REDUCTASE) (TSAR) from Escherichia coli strain K12 (294 aa), FASTA scores: opt: 469, E(): 6.7e-22, (34.4% identity in 282 aa overlap); P28811|MMSB_PSEAE 3-hydroxyisobutyrate dehydrogenase (HIBADH) from Pseudomonas aeruginosa (298 aa), FASTA scores: opt: 439, E(): 4.3e-20, (34.9% identity in 269 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv1122 and Rv1844c. SEEMS TO BELONG TO THE 3-HYDROXYISOBUTYRATE DEHYDROGENASE FAMILY. Mb0793 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XYH6" /db_xref="InterPro:IPR002204" /db_xref="InterPro:IPR006115" /db_xref="InterPro:IPR008927" /db_xref="InterPro:IPR013328" /db_xref="InterPro:IPR015815" /db_xref="InterPro:IPR029154" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/TrEMBL:A0A1R3XYH6" BEGIN 1 MTAHPETPRL GYIGLGNQGA PMAKRLLDWP GGLTVFDVRV EAMAPFVEGG ATAAASVSDV 61 AEADIISITV FDDAQVSSVI TADNGLATHA KPGTIVAIHS TIADTTAVDL AEKLKPQGIH 121 IVDAPGSGGA AAAAKGELAV MVGADDEAFQ RIKEPFSRWA SLLIHAGEPG AGTRMKLARN 181 MLTFVSYAAA AEAQRLAEAC GLDLVALGKV VRHSDSFTGG AGAIMFRNTT APMEPADPLR 241 PLLEHTRGLG EKDLSLALAL GEVVSVDLPL AQLALQRLAA GLGVPHPDTE PAKET //