LOCUS SIT99386.1 451 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CYTOCHROME P450 51 CYP51 (CYPL1) (P450-L1A1) (STEROL 14-ALPHA DEMETHYLASE) (LANOSTEROL 14-ALPHA DEMETHYLASE) (P450-14DM) protein. ACCESSION LT708304-798 PROTEIN_ID SIT99386.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp51" /locus_tag="BQ2027_MB0787C" /note="Mb0787c, cyp51, len: 451 aa. Equivalent to Rv0764c, len: 451 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 451 aa overlap). cyp51, cytochrome P450 51 (sterol 14-alpha demethylase) (EC 1.14.14.-), similar to others e.g. Q16850|CP51_HUMAN CYTOCHROME P450 51 (CYPL1) (P450L1) (STEROL 14-ALPHA DEMETHYLASE) (LANOSTEROL 14-ALPHA DEMETHYLASE) from Homo sapiens (509 aa), FASTA scores: opt: 848, E(): 0, (33.9% identity in 439 aa overlap); NP_172633.1|NC_003070 putative obtusifoliol 14-alpha demethylase from Arabidopsis thaliana (488 aa); P93596|CP51_WHEAT CYTOCHROME P450 51 (CYPL1) (P450-L1A1) (OBTUSIFOLIOL 14-ALPHA DEMETHYLASE) from Triticum aestivum (453 aa); etc. Also similar to many other Mycobacterium tuberculosis cytochromes P450 e.g. Rv1394c, FASTA score: (22.5% identity in 444 aa overlap). Contains PS00086 Cytochrome P450 cysteine heme-iron ligand signature. BELONGS TO THE CYTOCHROME P450 FAMILY. Protein product from Mb0787c detected using SWATH mass spectrometry. Mb0787c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A513" /db_xref="InterPro:IPR001128" /db_xref="InterPro:IPR002403" /db_xref="InterPro:IPR017972" /db_xref="InterPro:IPR036396" /db_xref="UniProtKB/Swiss-Prot:P0A513" BEGIN 1 MSAVALPRVS GGHDEHGHLE EFRTDPIGLM QRVRDECGDV GTFQLAGKQV VLLSGSHANE 61 FFFRAGDDDL DQAKAYPFMT PIFGEGVVFD ASPERRKEML HNAALRGEQM KGHAATIEDQ 121 VRRMIADWGE AGEIDLLDFF AELTIYTSSA CLIGKKFRDQ LDGRFAKLYH ELERGTDPLA 181 YVDPYLPIES FRRRDEARNG LVALVADIMN GRIANPPTDK SDRDMLDVLI AVKAETGTPR 241 FSADEITGMF ISMMFAGHHT SSGTASWTLI ELMRHRDAYA AVIDELDELY GDGRSVSFHA 301 LRQIPQLENV LKETLRLHPP LIILMRVAKG EFEVQGHRIH EGDLVAASPA ISNRIPEDFP 361 DPHDFVPARY EQPRQEDLLN RWTWIPFGAG RHRCVGAAFA IMQIKAIFSV LLREYEFEMA 421 QPPESYRNDH SKMVVQLAQP ACVRYRRRTG V //