LOCUS       SIT99386.1               451 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 CYTOCHROME
            P450 51 CYP51 (CYPL1) (P450-L1A1) (STEROL 14-ALPHA DEMETHYLASE)
            (LANOSTEROL 14-ALPHA DEMETHYLASE) (P450-14DM) protein.
ACCESSION   LT708304-798
PROTEIN_ID  SIT99386.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="cyp51"
                     /locus_tag="BQ2027_MB0787C"
                     /note="Mb0787c, cyp51, len: 451 aa. Equivalent to Rv0764c,
                     len: 451 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 451 aa overlap). cyp51, cytochrome
                     P450 51 (sterol 14-alpha demethylase) (EC 1.14.14.-),
                     similar to others e.g. Q16850|CP51_HUMAN CYTOCHROME P450
                     51 (CYPL1) (P450L1) (STEROL 14-ALPHA DEMETHYLASE)
                     (LANOSTEROL 14-ALPHA DEMETHYLASE) from Homo sapiens (509
                     aa), FASTA scores: opt: 848, E(): 0, (33.9% identity in
                     439 aa overlap); NP_172633.1|NC_003070 putative
                     obtusifoliol 14-alpha demethylase from Arabidopsis
                     thaliana (488 aa); P93596|CP51_WHEAT CYTOCHROME P450 51
                     (CYPL1) (P450-L1A1) (OBTUSIFOLIOL 14-ALPHA DEMETHYLASE)
                     from Triticum aestivum (453 aa); etc. Also similar to many
                     other Mycobacterium tuberculosis cytochromes P450 e.g.
                     Rv1394c, FASTA score: (22.5% identity in 444 aa overlap).
                     Contains PS00086 Cytochrome P450 cysteine heme-iron ligand
                     signature. BELONGS TO THE CYTOCHROME P450 FAMILY. Protein
                     product from Mb0787c detected using SWATH mass
                     spectrometry. Mb0787c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P0A513"
                     /db_xref="InterPro:IPR001128"
                     /db_xref="InterPro:IPR002403"
                     /db_xref="InterPro:IPR017972"
                     /db_xref="InterPro:IPR036396"
                     /db_xref="UniProtKB/Swiss-Prot:P0A513"
BEGIN
        1 MSAVALPRVS GGHDEHGHLE EFRTDPIGLM QRVRDECGDV GTFQLAGKQV VLLSGSHANE
       61 FFFRAGDDDL DQAKAYPFMT PIFGEGVVFD ASPERRKEML HNAALRGEQM KGHAATIEDQ
      121 VRRMIADWGE AGEIDLLDFF AELTIYTSSA CLIGKKFRDQ LDGRFAKLYH ELERGTDPLA
      181 YVDPYLPIES FRRRDEARNG LVALVADIMN GRIANPPTDK SDRDMLDVLI AVKAETGTPR
      241 FSADEITGMF ISMMFAGHHT SSGTASWTLI ELMRHRDAYA AVIDELDELY GDGRSVSFHA
      301 LRQIPQLENV LKETLRLHPP LIILMRVAKG EFEVQGHRIH EGDLVAASPA ISNRIPEDFP
      361 DPHDFVPARY EQPRQEDLLN RWTWIPFGAG RHRCVGAAFA IMQIKAIFSV LLREYEFEMA
      421 QPPESYRNDH SKMVVQLAQP ACVRYRRRTG V
//