LOCUS SIT99374.1 510 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE MMSA (METHYLMALONIC ACID SEMIALDEHYDE DEHYDROGENASE) (MMSDH) protein. ACCESSION LT708304-786 PROTEIN_ID SIT99374.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mmsA" /locus_tag="BQ2027_MB0775C" /note="Mb0775c, mmsA, len: 510 aa. Equivalent to Rv0753c, len: 510 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 510 aa overlap). Probable mmsA, methylmalonic acid semialdehyde dehydrogenase (EC 1.2.1.27), highly similar to others e.g. NP_420115.1|NC_002696 putative methylmalonate-semialdehyde dehydrogenase from Caulobacter crescentus (499 aa); L48550|STMMSDA_1|CAB75315.1|AL139164 methylmalonic acid semialdehyde dehydrogenase from Streptomyces coelicolor (500 aa), FASTA score: (51.6% identity in 498 aa overlap); M84911|PSEMMSRAB_2|NP_252260.1|NC_002516 methylmalonate-semialdehyde dehydrogenase from Pseudomonas aeruginosa (497 aa), FASTA scores: opt: 1127, E(): 0, (47.9% identity in 507 aa overlap); etc. Note that also highly similar to malonic semialdehyde oxidative decarboxylases e.g. NP_104968.1|NC_002678 malonic semialdehyde oxidative decarboxylase from Mesorhizobium loti (498 aa); NP_384832.1|NC_003047 PUTATIVE MALONIC SEMIALDEHYDE OXIDATIVE DECARBOXYLASE PROTEIN from Sinorhizobium meliloti (498 aa); etc. Contains PS00070 Aldehyde dehydrogenases cysteine active site. BELONGS TO THE ALDEHYDE DEHYDROGENASES FAMILY. Protein product from Mb0775c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0775c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XWE3" /db_xref="InterPro:IPR010061" /db_xref="InterPro:IPR015590" /db_xref="InterPro:IPR016160" /db_xref="InterPro:IPR016161" /db_xref="InterPro:IPR016162" /db_xref="InterPro:IPR016163" /db_xref="UniProtKB/TrEMBL:A0A1R3XWE3" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTTQISHFID GQRTAGQSTR SADVFDPNTG QIQAKVPMAG KSDIDAAVAS AVEAQKGWAA 61 WNPQRRARVL MRFIELVNDT IDELAELLSR EHGKTLADAR GDVQRGIEVI EFCLGIPHLL 121 KGEYTEGAGP GIDVYSLRQP LGVVAGITPF NFPAMIPLWK AGPALACGNA FVLKPSERDP 181 SVPVRLAELF IEAGLPAGVF QVVHGDKEAV DAILHHPDIK AVGFVGSSDI AQYIYAGAAA 241 TGKRAQCFGG AKNHMIVMPD ADLDQAVDAL IGAGYGSAGE RCMAISVAVP VGDQTAERLR 301 ARLIERINNL RVGHSLDPKA DYGPLVTGAA LARVRDYIGQ GVAAGAELVV DGRDRASDDL 361 TFGLPEGDAN LEGGFFIGPT LFDHVAAHMS IYTDEIFGPV LCMVRARDYE EALRLPSEHE 421 YGNGVAIFTR DGDAARDFVS RGQVGMVGVN VPIPVPVAYH TFGGWKRSGF GDLNQHGPAA 481 IQFYTKVKTV TSRWPSGIKD GAEFVIPTMS //