LOCUS SIT99349.1 448 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE D-XYLULOSE KINASE XYLB (XYLULOKINASE) (XYLULOSE KINASE) protein. ACCESSION LT708304-761 PROTEIN_ID SIT99349.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="xylB" /locus_tag="BQ2027_MB0750" /note="Mb0750, xylB, len: 448 aa. Equivalent to Rv0729, len: 448 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 448 aa overlap). Possible xylB, D-xylulose-kinase (xylulokinase) (EC 2.7.1.17). C-terminus highly similar to AAD09880.1|U77912 unknown protein from Mycobacterium bovis (102 aa); and N-terminus highly similar to T45387|Z98756|MLCB2492_25 hypothetical protein from Mycobacterium leprae (110 aa), FASTA scores: opt: 427, E(): 1.1e-19, (60.9% identity in 110 aa overlap). Also similar to xylA/xylB genes from various bacterial species e.g. AAC26499.1|AF045245 D-xylulose-kinase from Klebsiella pneumoniae (487 aa); NP_418021.1|NC_000913 xylulokinase from Escherichia coli strain K12 (484 aa), FASTA scores: opt: 260, E(): 7.5e-09, (25.9% identity in 478 aa overlap); etc. Also similar to Rv3696c|glpK PROBABLE GLYCEROL KINASE (EC 2.7.1.30) from Mycobacterium tuberculosis (517 aa). BELONGS TO THE FUCOKINASE / GLUCONOKINASE / GLYCEROKINASE / XYLULOKINASE FAMILY. Protein product from Mb0750 detected using SWATH mass spectrometry. Mb0750 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XW81" /db_xref="InterPro:IPR000577" /db_xref="InterPro:IPR018484" /db_xref="InterPro:IPR018485" /db_xref="UniProtKB/TrEMBL:A0A1R3XW81" BEGIN 1 MSRDDVTIGI DIGTTAVKAV AADDNGRVTA RVRIGHQLAV PAPDRLEHDA DEAWRRGPLA 61 ALDRLVGPDT RALAVAAMVP SLTAVDPAGR PITPGLLYGD ARGRVPNASV ARAQSVPSVG 121 ETAEFLRWTA GQAPDASGYW PAPAVANYAL SGEAVIDYAT AVTTLPLFDG TGWNATACAD 181 CGVTVDRMPR VETFGVGVGQ VRGTGAVLAV GAVDALCEQI VAGADRDGDV LVLCGATLIV 241 WTTISAARQV PGLWTIPHTA PGKSQIGGAS NAGGLFLNWV DRVIGPGDPA LADPRRVPVW 301 LPYIRGERTP FHEPDRRAVL DGVDLSQDAA SVRRAAYEAS GFVVRQLIEL SGAPVARIVA 361 AGGGTRIQPW MQAIADATGR PVEVSRVAEG AALGAAFLGR LAAGLESSIA DAARWASTDR 421 IVEPSADWAG PTKERYRRFL ALSGSKLA //