LOCUS SIT99331.1 636 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ARYLSULFATASE ATSAb [SECOND PART] (ARYL-SULFATE SULPHOHYDROLASE) (ARYLSULPHATASE) protein. ACCESSION LT708304-743 PROTEIN_ID SIT99331.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="atsAb" /locus_tag="BQ2027_MB0732" /note="Mb0732, atsAb, len: 636 aa. Equivalent to 3' end of Rv0711, len: 787 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 636 aa overlap). Possible atsA, arylsulfatase (EC 3.1.6.1), similar to others e.g. P51691|ARS_PSEAE arylsulfatase from Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439, E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc. Also similar to other hypothetical arylsulfatases from Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc. Contains PS00523 Sulfatases signature 1, and PS00149 Sulfatases signature 2. BELONGS TO THE SULFATASE FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, atsA exists as a single gene. In Mycobacterium bovis, a single base transversion (g-t), introducing a stop codon, splits atsA into 2 parts, atsAa and atsAb. Protein product from Mb0732 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0732 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XW65" /db_xref="InterPro:IPR000917" /db_xref="InterPro:IPR017850" /db_xref="UniProtKB/TrEMBL:A0A1R3XW65" BEGIN 1 MASTKRHWPT SRGFERFYGF LGGETDQWYP DLVYDNHPVS PPGTPEGGYH LSKDIADKTI 61 EFIRDAKVIA PDKPWFSYVC PGAGHAPHHV FKEWADRYAG RFDMGYERYR EIVLERQKAL 121 GIVPPDTELS PINPYLDVPG PNGETWPLQD TVRPWDSLSD EEKKLFCRMA EVFAGFLSYT 181 DAQIGRILDY LEESGQLDNT IIVVISDNGA SGEGGPNGSV NEGKFFNGYI DTVAESMKLF 241 DHLGGPQTYN HYPIGWAMAF NTPYKLFKRY ASHEGGIADP AIISWPNGIA AHGEIRDNYV 301 NVSDITPTVY DLLGMTPPGT VKGIPQKPMD GVSFIAALAD PAADTGKTTQ FYTMLGTRGI 361 WHEGWFANTI HAATPAGWSN FNADRWELFH IAADRSQCHD LAAEHPDKLE ELKALWFSEA 421 AKYNGLPLAD LNLLETMTRS RPYLVSERAS YVYYPDCADV GIGAAVEIRG RSFAVLADVT 481 IDTTGAEGVL FKHGGAHGGH VLFVRDGRLH YVYNFLGERQ QLVSSSGPVP SGRHLLGVRY 541 LRTGTVPNSH TPVGDLELFF DENLVGALTN VLTHPGTFGL AGAAISVGRN GGSAVSSHYE 601 APFAFTGGTI TQVTVDVSGR PFEDVESDLA LAFSRD //