LOCUS       SIT99331.1               636 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            ARYLSULFATASE ATSAb [SECOND PART] (ARYL-SULFATE SULPHOHYDROLASE)
            (ARYLSULPHATASE) protein.
ACCESSION   LT708304-743
PROTEIN_ID  SIT99331.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="atsAb"
                     /locus_tag="BQ2027_MB0732"
                     /note="Mb0732, atsAb, len: 636 aa. Equivalent to 3' end of
                     Rv0711, len: 787 aa, from Mycobacterium tuberculosis
                     strain H37Rv, (100.0% identity in 636 aa overlap).
                     Possible atsA, arylsulfatase (EC 3.1.6.1), similar to
                     others e.g. P51691|ARS_PSEAE arylsulfatase from
                     Pseudomonas aeruginosa (532 aa), FASTA scores: opt: 439,
                     E(): 2.9e-21, (30.8% identity in 552 aa overlap); etc.
                     Also similar to other hypothetical arylsulfatases from
                     Mycobacterium tuberculosis e.g. Rv3299c, Rv0663, etc.
                     Contains PS00523 Sulfatases signature 1, and PS00149
                     Sulfatases signature 2. BELONGS TO THE SULFATASE FAMILY.
                     REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, atsA exists as a single gene.
                     In Mycobacterium bovis, a single base transversion (g-t),
                     introducing a stop codon, splits atsA into 2 parts, atsAa
                     and atsAb. Protein product from Mb0732 detected using
                     shotgun mass spectrometry and SWATH mass spectrometry.
                     Mb0732 found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XW65"
                     /db_xref="InterPro:IPR000917"
                     /db_xref="InterPro:IPR017850"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XW65"
BEGIN
        1 MASTKRHWPT SRGFERFYGF LGGETDQWYP DLVYDNHPVS PPGTPEGGYH LSKDIADKTI
       61 EFIRDAKVIA PDKPWFSYVC PGAGHAPHHV FKEWADRYAG RFDMGYERYR EIVLERQKAL
      121 GIVPPDTELS PINPYLDVPG PNGETWPLQD TVRPWDSLSD EEKKLFCRMA EVFAGFLSYT
      181 DAQIGRILDY LEESGQLDNT IIVVISDNGA SGEGGPNGSV NEGKFFNGYI DTVAESMKLF
      241 DHLGGPQTYN HYPIGWAMAF NTPYKLFKRY ASHEGGIADP AIISWPNGIA AHGEIRDNYV
      301 NVSDITPTVY DLLGMTPPGT VKGIPQKPMD GVSFIAALAD PAADTGKTTQ FYTMLGTRGI
      361 WHEGWFANTI HAATPAGWSN FNADRWELFH IAADRSQCHD LAAEHPDKLE ELKALWFSEA
      421 AKYNGLPLAD LNLLETMTRS RPYLVSERAS YVYYPDCADV GIGAAVEIRG RSFAVLADVT
      481 IDTTGAEGVL FKHGGAHGGH VLFVRDGRLH YVYNFLGERQ QLVSSSGPVP SGRHLLGVRY
      541 LRTGTVPNSH TPVGDLELFF DENLVGALTN VLTHPGTFGL AGAAISVGRN GGSAVSSHYE
      601 APFAFTGGTI TQVTVDVSGR PFEDVESDLA LAFSRD
//