LOCUS SIT99302.1 396 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable iron-regulated elongation factor tu tuf (ef-tu) protein. ACCESSION LT708304-714 PROTEIN_ID SIT99302.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tuf" /locus_tag="BQ2027_MB0704" /note="Mb0704, tuf, len: 396 aa. Equivalent to Rv0685, len: 396 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 396 aa overlap). Probable tuf, elongation factor EF-Tu, equivalent to JC2262 translation elongation factor Tu from Mycobacterium leprae (396 aa). Also highly similar to others e.g. P42439|EFTU_CORGL ELONGATION FACTOR TU (EF-TU) from Corynebacterium glutamicum (396 aa); etc. Contains PS00017 ATP/GTP-binding site motif A, and PS00301 GTP-binding elongation factors signature. BELONGS TO THE GTP-BINDING ELONGATION FACTOR FAMILY, EF-TU/EF-1A SUBFAMILY. Protein product from Mb0704 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0704 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A559" /db_xref="InterPro:IPR000795" /db_xref="InterPro:IPR004160" /db_xref="InterPro:IPR004161" /db_xref="InterPro:IPR004541" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR009000" /db_xref="InterPro:IPR009001" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR031157" /db_xref="InterPro:IPR033720" /db_xref="InterPro:IPR041709" /db_xref="UniProtKB/Swiss-Prot:P0A559" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MAKAKFQRTK PHVNIGTIGH VDHGKTTLTA AITKVLHDKF PDLNETKAFD QIDNAPEERQ 61 RGITINIAHV EYQTDKRHYA HVDAPGHADY IKNMITGAAQ MDGAILVVAA TDGPMPQTRE 121 HVLLARQVGV PYILVALNKA DAVDDEELLE LVEMEVRELL AAQEFDEDAP VVRVSALKAL 181 EGDAKWVASV EELMNAVDES IPDPVRETDK PFLMPVEDVF TITGRGTVVT GRVERGVINV 241 NEEVEIVGIR PSTTKTTVTG VEMFRKLLDQ GQAGDNVGLL LRGVKREDVE RGQVVTKPGT 301 TTPHTEFEGQ VYILSKDEGG RHTPFFNNYR PQFYFRTTDV TGVVTLPEGT EMVMPGDNTN 361 ISVKLIQPVA MDEGLRFAIR EGGRTVGAGR VTKIIK //