LOCUS SIT99298.1 196 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN (POSSIBLY TETR-FAMILY) protein. ACCESSION LT708304-710 PROTEIN_ID SIT99298.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0700" /note="Mb0700, -, len: 196 aa. Equivalent to Rv0681, len: 196 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 196 aa overlap). Probable transcription regulator, TetR family, similar to others and especially many tetracycline repressors e.g. T34657 probable transcription regulator from Streptomyces coelicolor (189 aa); AF0278|AF027868_40|NP_389788.1|NC_000964 yobS regulator from Bacillus subtilis (191 aa), FASTA scores: opt: 213, E(): 1.6e-07, (28.8% identity in 153 aa overlap); P09164|TER4_ECOLI TETRACYCLINE REPRESSOR PROTEIN from Escherichia coli (217 aa), FASTA scores: opt: 145, E(): 0.0068, (39.0% identity in 59 aa overlap); etc. Contains helix-turn-helix motif at aa 28-49 (Score 1020, +2.66 SD). Protein product from Mb0700 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0700 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XW36" /db_xref="InterPro:IPR001647" /db_xref="InterPro:IPR009057" /db_xref="InterPro:IPR025996" /db_xref="InterPro:IPR036271" /db_xref="UniProtKB/TrEMBL:A0A1R3XW36" BEGIN 1 MARPAKLSRE SIVEGALTFL DREGWDSLTI NALATQLGTK GPSLYNHVDS LEDLRRAVRI 61 RVIDDIITML NRVGAGRARD DAVLVMAGAY RSYAHHHPGR YSAFTRMPLG GDDPEYTAAT 121 RGAAAPVIAV LSSYGLDGEQ AFYAALEFWS ALHGFVLLEM TGVMDDIDTD AVFTDMVLRL 181 AAGMERRTTH GGTAST //