LOCUS SIT99293.1 964 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED TRANSMEMBRANE TRANSPORT PROTEIN MMPL5 protein. ACCESSION LT708304-705 PROTEIN_ID SIT99293.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mmpL5" /locus_tag="BQ2027_MB0695C" /note="Mb0695c, mmpL5, len: 964 aa. Equivalent to Rv0676c, len: 964 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 964 aa overlap). Probable mmpL5, conserved transmembrane transport protein (see first citation below), member of RND superfamily, highly similar to other Mycobacterial proteins e.g. MTV037_14, MTCY98_8, MTCY20G9_34, MTCY4D9_15, MTCY48_8, MTCY19G5_6, MTV005_19, etc. Also similar to other Mycobacterial mmpl proteins e.g. P54881|MML4_MYCLE PUTATIVE MEMBRANE PROTEIN MMPL4 from Mycobacterium leprae (959 aa), FASTA scores: opt: 3991, E(): 0, (62.8% identity in 933 aa overlap); etc. BELONGS TO THE MMPL FAMILY. TBparse score is 0.884. Protein product from Mb0695c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0695c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:A0A1R3XX34" /db_xref="InterPro:IPR004707" /db_xref="InterPro:IPR004869" /db_xref="UniProtKB/TrEMBL:A0A1R3XX34" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MIVQRTAAPT GSVPPDRHAA RPFIPRMIRT FAVPIILGWL VTIAVLNVTV PQLETVGQIQ 61 AVSMSPDAAP SMISMKHIGK VFEEGDSDSA AMIVLEGQRP LGDAAHAFYD QMIGRLQADT 121 THVQSLQDFW GDPLTATGAQ SSDGKAAYVQ VKLAGNQGES LANESVEAVK TIVERLAPPP 181 GVKVYVTGSA ALVADQQQAG DRSLQVIEAV TFTVIIVMLL LVYRSIITSA IMLTMVVLGL 241 LATRGGVAFL GFHRIIGLST FATNLLVVLA IAAATDYAIF LIGRYQEARG LGQDRESAYY 301 TMFGGTAHVV LGSGLTIAGA TFCLSFTRLP YFQTLGVPLA IGMVIVVAAA LTLGPAIIAV 361 TSRFGKLLEP KRMARVRGWR KVGAAIVRWP GPILVGAVAL ALVGLLTLPG YRTNYNDRNY 421 LPADLPANEG YAAAERHFSQ ARMNPEVLMV ESDHDMRNSA DFLVINKIAK AIFAVEGISR 481 VQAITRPDGK PIEHTSIPFL ISMQGTSQKL TEKYNQDLTA RMLEQVNDIQ SNIDQMERMH 541 SLTQQMADVT HEMVIQMTGM VVDVEELRNH IADFDDFFRP IRSYFYWEKH CYDIPVCWSL 601 RSVFDTLDGI DVMTEDINNL LPLMQRLDTL MPQLTAMMPE MIQTMKSMKA QMLSMHSTQE 661 GLQDQMAAMQ EDSAAMGEAF DASRNDDSFY LPPEVFDNPD FQRGLEQFLS PDGHAVRFII 721 SHEGDPMSQA GIARIAKIKT AAKEAIKGTP LEGSAIYLGG TAAMFKDLSD GNTYDLMIAG 781 ISALCLIFII MLIITRSVVA AAVIVGTVVL SLGASFGLSV LIWQHILGIE LHWLVLAMAV 841 IILLAVGADY NLLLVARLKE EIHAGINTGI IRAMGGSGSV VTAAGLVFAF TMMSFAVSEL 901 TVMAQVGTTI GMGLLFDTLI VRSFMTPSIA ALLGKWFWWP QVVRQRPVPQ PWPSPASART 961 FALV //