LOCUS       SIT99292.1               263 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ENOYL-COA HYDRATASE ECHA5 (ENOYL HYDRASE) (UNSATURATED
            ACYL-COA HYDRATASE) (CROTONASE) protein.
ACCESSION   LT708304-704
PROTEIN_ID  SIT99292.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="echA5"
                     /locus_tag="BQ2027_MB0694"
                     /note="Mb0694, echA5, len: 263 aa. Equivalent to Rv0675,
                     len: 263 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 263 aa overlap). Probable echA5,
                     enoyl-CoA hydratase (EC 4.2.1.17), similar to several e.g.
                     NP_252116.1|NC_002516 probable enoyl
                     CoA-hydratase/isomerase from Pseudomonas aeruginosa (256
                     aa); Q20376 PROTEIN SIMILAR TO ENOYL-COA HYDRATASE from
                     Caenorhabditis elegans (258 aa), FASTA scores: opt: 697,
                     E(): 0, (47.3% identity in 245 aa overlap); etc. Also
                     similar to others from Mycobacterium tuberculosis e.g.
                     Z92669|MTCY8D5_17 (262 aa), FASTA scores: opt: 493, E():
                     3.6e-25, (39.1% identity in 243 aa overlap); etc. Protein
                     product from Mb0694 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0694 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:A0A1R3XY72"
                     /db_xref="InterPro:IPR001753"
                     /db_xref="InterPro:IPR018376"
                     /db_xref="InterPro:IPR029045"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XY72"
BEGIN
        1 MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF
       61 CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA
      121 EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP
      181 KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA
      241 RRFAAGAGRH GAPAPRAEQG DTL
//