LOCUS SIT99292.1 263 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ENOYL-COA HYDRATASE ECHA5 (ENOYL HYDRASE) (UNSATURATED ACYL-COA HYDRATASE) (CROTONASE) protein. ACCESSION LT708304-704 PROTEIN_ID SIT99292.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="echA5" /locus_tag="BQ2027_MB0694" /note="Mb0694, echA5, len: 263 aa. Equivalent to Rv0675, len: 263 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 263 aa overlap). Probable echA5, enoyl-CoA hydratase (EC 4.2.1.17), similar to several e.g. NP_252116.1|NC_002516 probable enoyl CoA-hydratase/isomerase from Pseudomonas aeruginosa (256 aa); Q20376 PROTEIN SIMILAR TO ENOYL-COA HYDRATASE from Caenorhabditis elegans (258 aa), FASTA scores: opt: 697, E(): 0, (47.3% identity in 245 aa overlap); etc. Also similar to others from Mycobacterium tuberculosis e.g. Z92669|MTCY8D5_17 (262 aa), FASTA scores: opt: 493, E(): 3.6e-25, (39.1% identity in 243 aa overlap); etc. Protein product from Mb0694 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0694 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XY72" /db_xref="InterPro:IPR001753" /db_xref="InterPro:IPR018376" /db_xref="InterPro:IPR029045" /db_xref="UniProtKB/TrEMBL:A0A1R3XY72" BEGIN 1 MSDLVRVERK GRVTTVILNR PASRNAVNGP TAAALCAAFE QFDRDDAASV AVLWGAGGTF 61 CAGADLKAFG TPEANSVHRT GPGPMGPSRM MLSKPVIAAV SGYAVAGGLE LALWCDLRVA 121 EEDAVFGVFC RRWGVPLIDG GTVRLPRLIG HSRAMDMILT GRGVPADEAL AMGLANRVVP 181 KGQARQAAEE LAAQLAALPQ QCLRSDRLSA LHQWGLPESA ALDLEFASIA RVAGEALEGA 241 RRFAAGAGRH GAPAPRAEQG DTL //