LOCUS SIT99284.1 1172 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 DNA- DIRECTED RNA POLYMERASE (BETA CHAIN) RPOB (TRANSCRIPTASE BETA CHAIN) (RNA POLYMERASE BETA SUBUNIT) protein. ACCESSION LT708304-696 PROTEIN_ID SIT99284.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="rpoB" /locus_tag="BQ2027_MB0686" /note="Mb0686, rpoB, len: 1172 aa. Equivalent to Rv0667, len: 1172 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 1172 aa overlap). rpoB, DNA-directed RNA polymerase, beta chain (EC 2.7.7.6) (see first and third citations below), equivalent to P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta chain from Mycobacterium leprae (1178 aa). Also highly similar to others e.g. AAF60349.1|AF242549_1|AF242549 DNA-dependent RNA polymerase beta subunit from Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431 DNA-directed RNA polymerase beta chain from Streptomyces coelicolor (1161 aa); etc. Start site chosen on basis of RBS but alternative start exists at position 14359. BELONGS TO THE RNA POLYMERASE BETA CHAIN FAMILY. Protein product from Mb0686 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0686 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P0A681" /db_xref="InterPro:IPR007120" /db_xref="InterPro:IPR007121" /db_xref="InterPro:IPR007641" /db_xref="InterPro:IPR007642" /db_xref="InterPro:IPR007644" /db_xref="InterPro:IPR007645" /db_xref="InterPro:IPR010243" /db_xref="InterPro:IPR014724" /db_xref="InterPro:IPR015712" /db_xref="InterPro:IPR019462" /db_xref="InterPro:IPR037033" /db_xref="InterPro:IPR037034" /db_xref="InterPro:IPR042107" /db_xref="UniProtKB/Swiss-Prot:P0A681" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MADSRQSKTA ASPSPSRPQS SSNNSVPGAP NRVSFAKLRE PLEVPGLLDV QTDSFEWLIG 61 SPRWRESAAE RGDVNPVGGL EEVLYELSPI EDFSGSMSLS FSDPRFDDVK APVDECKDKD 121 MTYAAPLFVT AEFINNNTGE IKSQTVFMGD FPMMTEKGTF IINGTERVVV SQLVRSPGVY 181 FDETIDKSTD KTLHSVKVIP SRGAWLEFDV DKRDTVGVRI DRKRRQPVTV LLKALGWTSE 241 QIVERFGFSE IMRSTLEKDN TVGTDEALLD IYRKLRPGEP PTKESAQTLL ENLFFKEKRY 301 DLARVGRYKV NKKLGLHVGE PITSSTLTEE DVVATIEYLV RLHEGQTTMT VPGGVEVPVE 361 TDDIDHFGNR RLRTVGELIQ NQIRVGMSRM ERVVRERMTT QDVEAITPQT LINIRPVVAA 421 IKEFFGTSQL SQFMDQNNPL SGLTHKRRLS ALGPGGLSRE RAGLEVRDVH PSHYGRMCPI 481 ETPEGPNIGL IGSLSVYARV NPFGFIETPY RKVVDGVVSD EIVYLTADEE DRHVVAQANS 541 PIDADGRFVE PRVLVRRKAG EVEYVPSSEV DYMDVSPRQM VSVATAMIPF LEHDDANRAL 601 MGANMQRQAV PLVRSEAPLV GTGMELRAAI DAGDVVVAEE SGVIEEVSAD YITVMHDNGT 661 RRTYRMRKFA RSNHGTCANQ CPIVDAGDRV EAGQVIADGP CTDDGEMALG KNLLVAIMPW 721 EGHNYEDAII LSNRLVEEDV LTSIHIEEHE IDARDTKLGA EEITRDIPNI SDEVLADLDE 781 RGIVRIGAEV RDGDILVGKV TPKGETELTP EERLLRAIFG EKAREVRDTS LKVPHGESGK 841 VIGIRVFSRE DEDELPAGVN ELVRVYVAQK RKISDGDKLA GRHGNKGVIG KILPVEDMPF 901 LADGTPVDII LNTHGVPRRM NIGQILETHL GWCAHSGWKV DAAKGVPDWA ARLPDELLEA 961 QPNAIVSTPV FDGAQEAELQ GLLSCTLPNR DGDVLVDADG KAMLFDGRSG EPFPYPVTVG 1021 YMYIMKLHHL VDDKIHARST GPYSMITQQP LGGKAQFGGQ RFGEMECWAM QAYGAAYTLQ 1081 ELLTIKSDDT VGRVKVYEAI VKGENIPEPG IPESFKVLLK ELQSLCLNVE VLSSDGAAIE 1141 LREGEDEDLE RAAANLGINL SRNESASVED LA //