LOCUS       SIT99284.1              1172 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 DNA-
            DIRECTED RNA POLYMERASE (BETA CHAIN) RPOB (TRANSCRIPTASE
            BETA CHAIN) (RNA POLYMERASE BETA SUBUNIT) protein.
ACCESSION   LT708304-696
PROTEIN_ID  SIT99284.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="rpoB"
                     /locus_tag="BQ2027_MB0686"
                     /note="Mb0686, rpoB, len: 1172 aa. Equivalent to Rv0667,
                     len: 1172 aa, from Mycobacterium tuberculosis strain
                     H37Rv, (100.0% identity in 1172 aa overlap). rpoB,
                     DNA-directed RNA polymerase, beta chain (EC 2.7.7.6) (see
                     first and third citations below), equivalent to
                     P30760|RPOB_MYCLE|ML1891 DNA-directed RNA polymerase beta
                     chain from Mycobacterium leprae (1178 aa). Also highly
                     similar to others e.g. AAF60349.1|AF242549_1|AF242549
                     DNA-dependent RNA polymerase beta subunit from
                     Amycolatopsis mediterranei (1167 aa); CAB77428.1|AL160431
                     DNA-directed RNA polymerase beta chain from Streptomyces
                     coelicolor (1161 aa); etc. Start site chosen on basis of
                     RBS but alternative start exists at position 14359.
                     BELONGS TO THE RNA POLYMERASE BETA CHAIN FAMILY. Protein
                     product from Mb0686 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0686 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
                     /db_xref="GOA:P0A681"
                     /db_xref="InterPro:IPR007120"
                     /db_xref="InterPro:IPR007121"
                     /db_xref="InterPro:IPR007641"
                     /db_xref="InterPro:IPR007642"
                     /db_xref="InterPro:IPR007644"
                     /db_xref="InterPro:IPR007645"
                     /db_xref="InterPro:IPR010243"
                     /db_xref="InterPro:IPR014724"
                     /db_xref="InterPro:IPR015712"
                     /db_xref="InterPro:IPR019462"
                     /db_xref="InterPro:IPR037033"
                     /db_xref="InterPro:IPR037034"
                     /db_xref="InterPro:IPR042107"
                     /db_xref="UniProtKB/Swiss-Prot:P0A681"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MADSRQSKTA ASPSPSRPQS SSNNSVPGAP NRVSFAKLRE PLEVPGLLDV QTDSFEWLIG
       61 SPRWRESAAE RGDVNPVGGL EEVLYELSPI EDFSGSMSLS FSDPRFDDVK APVDECKDKD
      121 MTYAAPLFVT AEFINNNTGE IKSQTVFMGD FPMMTEKGTF IINGTERVVV SQLVRSPGVY
      181 FDETIDKSTD KTLHSVKVIP SRGAWLEFDV DKRDTVGVRI DRKRRQPVTV LLKALGWTSE
      241 QIVERFGFSE IMRSTLEKDN TVGTDEALLD IYRKLRPGEP PTKESAQTLL ENLFFKEKRY
      301 DLARVGRYKV NKKLGLHVGE PITSSTLTEE DVVATIEYLV RLHEGQTTMT VPGGVEVPVE
      361 TDDIDHFGNR RLRTVGELIQ NQIRVGMSRM ERVVRERMTT QDVEAITPQT LINIRPVVAA
      421 IKEFFGTSQL SQFMDQNNPL SGLTHKRRLS ALGPGGLSRE RAGLEVRDVH PSHYGRMCPI
      481 ETPEGPNIGL IGSLSVYARV NPFGFIETPY RKVVDGVVSD EIVYLTADEE DRHVVAQANS
      541 PIDADGRFVE PRVLVRRKAG EVEYVPSSEV DYMDVSPRQM VSVATAMIPF LEHDDANRAL
      601 MGANMQRQAV PLVRSEAPLV GTGMELRAAI DAGDVVVAEE SGVIEEVSAD YITVMHDNGT
      661 RRTYRMRKFA RSNHGTCANQ CPIVDAGDRV EAGQVIADGP CTDDGEMALG KNLLVAIMPW
      721 EGHNYEDAII LSNRLVEEDV LTSIHIEEHE IDARDTKLGA EEITRDIPNI SDEVLADLDE
      781 RGIVRIGAEV RDGDILVGKV TPKGETELTP EERLLRAIFG EKAREVRDTS LKVPHGESGK
      841 VIGIRVFSRE DEDELPAGVN ELVRVYVAQK RKISDGDKLA GRHGNKGVIG KILPVEDMPF
      901 LADGTPVDII LNTHGVPRRM NIGQILETHL GWCAHSGWKV DAAKGVPDWA ARLPDELLEA
      961 QPNAIVSTPV FDGAQEAELQ GLLSCTLPNR DGDVLVDADG KAMLFDGRSG EPFPYPVTVG
     1021 YMYIMKLHHL VDDKIHARST GPYSMITQQP LGGKAQFGGQ RFGEMECWAM QAYGAAYTLQ
     1081 ELLTIKSDDT VGRVKVYEAI VKGENIPEPG IPESFKVLLK ELQSLCLNVE VLSSDGAAIE
     1141 LREGEDEDLE RAAANLGINL SRNESASVED LA
//