LOCUS SIT99272.1 359 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE RIBONUCLEOTIDE-TRANSPORT ATP-BINDING PROTEIN ABC TRANSPORTER MKL protein. ACCESSION LT708304-684 PROTEIN_ID SIT99272.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mkl" /locus_tag="BQ2027_MB0674" /note="Mb0674, mkl, len: 359 aa. Equivalent to Rv0655, len: 359 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 359 aa overlap). Possible mkl, ribonucleotide-transport ATP-binding protein ABC transporter (see first citation below), equivalent to P30769|MKL_MYCLE|ML1892 POSSIBLE RIBONUCLEOTIDE TRANSPORT ATP-BINDING PROTEIN from Mycobacterium leprae (347 aa), FASTA scores: opt: 2021, E(): 0, (92.2% identity in 335 aa overlap). Also highly similar to many e.g. AB92896.1|AL356992 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (343 aa); NP_253146.1|NC_002516 probable ATP-binding component of ABC transporter from Pseudomonas aeruginosa (269 aa); P45393|YRBF_ECOLI hypothetical ABC transporter ATP-binding protein from Escherichia coli (269 aa), FASTA scores: opt: 644, E(): 3.4e-33, (38.5% identity in 244 aa overlap); etc. Also similar to many other Mycobacterium tuberculosis ABC transporters e.g. P71747|CYSA|Rv2397c|MTCY253.24 (351 aa), FASTA score: (33.6% identity in 241 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00211 ABC transporters family signature. BELONGS TO THE ATP-BINDING TRANSPORT PROTEIN FAMILY (ABC TRANSPORTERS). Protein product from Mb0674 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0674 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P63358" /db_xref="InterPro:IPR003439" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR017871" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR030296" /db_xref="UniProtKB/Swiss-Prot:P63358" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MRYSDSYHTT GRWQPRASTE GFPMGVSIEV NGLTKSFGSS RIWEDVTLTI PAGEVSVLLG 61 PSGTGKSVFL KSLIGLLRPE RGSIIIDGTD IIECSAKELY EIRTLFGVLF QDGALFGSMN 121 LYDNTAFPLR EHTKKKESEI RDIVMEKLAL VGLGGDEKKF PGEISGGMRK RAGLARALVL 181 DPQIILCDEP DSGLDPVRTA YLSQLIMDIN AQIDATILIV THNINIARTV PDNMGMLFRK 241 HLVMFGPREV LLTSDEPVVR QFLNGRRIGP IGMSEEKDEA TMAEEQALLD AGHHAGGVEE 301 IEGVPPQISA TPGMPERKAV ARRQARVREM LHTLPKKAQA AILDDLEGTH KYAVHEIGQ //