LOCUS SIT99271.1 501 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE DIOXYGENASE protein. ACCESSION LT708304-683 PROTEIN_ID SIT99271.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0673" /note="Mb0673, -, len: 501 aa. Equivalent to Rv0654, len: 501 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 501 aa overlap). Probable dioxygenase (EC 1.-.-.-), highly similar to others eg AAK06796.1|AF324838_15|AF324838|SimC5 putative dioxygenase (involved in tetraene formation) from Streptomyces antibioticus (456 aa); CAB56138.1| AL117669 putative dioxygenase from Streptomyces coelicolor (503 aa); T51734 neoxanthin cleavage enzyme (9-cis-epoxy-carotenoid dioxygenase) from Arabidopsis thaliana (538 aa); Q53353 LIGNOSTILBENE-ALPHA,BETA-DIOXYGENASE from Pseudomonas paucimobilis (Sphingomonas paucimobilis), FASTA scores: opt: 280, E(): 2.3e-11, (28.5% identity in 523 aa overlap); etc. Also some similarity with Rv0913c|MTCY21C12.07c POSSIBLE DIOXYGENASE from Mycobacterium tuberculosis (501 aa), FASTA score: (29.5% identity in 522 aa overlap). Protein product from Mb0673 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0673 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XW04" /db_xref="InterPro:IPR004294" /db_xref="UniProtKB/TrEMBL:A0A1R3XW04" BEGIN 1 MTTAQAAESQ NPYLEGFLAP VSTEVTATDL PVTGRIPEHL DGRYLRNGPN PVAEVDPATY 61 HWFTGDAMVH GVALRDGKAR WYRNRWVRTP AVCAALGEPI SARPHPRTGI IEGGPNTNVL 121 THAGRTLALV EAGVVNYELT DELDTVGPCD FDGTLHGGYT AHPQRDPHTG ELHAVSYSFA 181 RGHRVQYSVI GTDGHARRTV DIEVAGSPMM HSFSLTDNYV VIYDLPVTFD PMQVVPASVP 241 RWLQRPARLV IQSVLGRVRI PDPIAALGNR MQGHSDRLPY AWNPSYPARV GVMPREGGNE 301 DVRWFDIEPC YVYHPLNAYS ECRNGAEVLV LDVVRYSRMF DRDRRGPGGD SRPSLDRWTI 361 NLATGAVTAE CRDDRAQEFP RINETLVGGP HRFAYTVGIE GGFLVGAGAA LSTPLYKQDC 421 VTGSSTVASL DPDLLIGEMV FVPNPSARAE DDGILMGYGW HRGRDEGQLL LLDAQTLESI 481 ATVHLPQRVP MGFHGNWAPT T //