LOCUS SIT99252.1 158 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 (3r) -hydroxyacyl-acp dehydratase subunit hada protein. ACCESSION LT708304-664 PROTEIN_ID SIT99252.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="hadA" /locus_tag="BQ2027_MB0654" /note="Mb0654, -, len: 158 aa. Equivalent to Rv0635, len: 158 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 158 aa overlap). Conserved hypothetical protein, equivalent to NP_302287.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (159 aa); and highly similar to YV31_MYCLE|P54879 conserved hypothetical protein from Mycobacterium leprae (166 aa), FASTA scores: opt: 387, E(): 5.9e-21, (43.4% identity in 145 aa overlap). Also similar CAB77410.1|AL160431|SCD82.07 hypothetical protein from Streptomyces coelicolor (150 aa). And highly similar to two hypothetical proteins from Mycobacterium tuberculosis: Rv0504c|YV31_MYCTU|Q11168 (166 aa), FASTA scores: opt: 405, E(): 3.2e-22, (45.0% identity in 140 aa overlap); and Rv0637|MTY20H10_19 (2 ORFs downstream) (166 aa), FASTA score: (48.7% identity in 150 aa overlap). Protein product from Mb0654 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0654 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR016709" /db_xref="InterPro:IPR029069" /db_xref="InterPro:IPR039569" /db_xref="UniProtKB/Swiss-Prot:Q7U1K4" BEGIN 1 MALSADIVGM HYRYPDHYEV EREKIREYAV AVQNDDAWYF EEDGAAELGY KGLLAPLTFI 61 CVFGYKAQAA FFKHANIATA EAQIVQVDQV LKFEKPIVAG DKLYCDVYVD SVREAHGTQI 121 IVTKNIVTNE EGDLVQETYT TLAGRAGEDG EGFSDGAA //