LOCUS SIT98450.1 425 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 serine hydroxymethyltransferase glya2 (serine methylase 2) (shmt 2) protein. ACCESSION LT708304-72 PROTEIN_ID SIT98450.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="glyA2" /locus_tag="BQ2027_MB0071C" /note="Mb0071c, glyA2, len: 425 aa. Equivalent to Rv0070c, len: 425 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 425 aa overlap). Probable glyA2, serine hydroxymethyltransferase (EC 2.1.2.1), equivalent to NP_302318.1|NC_002677 serine hydroxymethyltransferase from Mycobacterium leprae (426 aa). Also highly similar to many e.g. O86565|GLYA_STRCO SERINE HYDROXYMETHYLTRANSFERASE from Streptomyces coelicolor (420 aa); AAK60516.1|AF327063_1|AF327063 serine hydroxymethyltransferase from Corynebacterium glutamicum (434 aa); GLYA_ECOLI|P00477 serine hydroxymethyltransferase from Escherichia coli (417 aa), FASTA scores: opt: 1462, E(): 0, (54.3% identity in 416 aa overlap); etc. Also highly similar to MTV017_46 from Mycobacterium tuberculosis. Contains PS00096 Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. BELONGS TO THE SHMT FAMILY. COFACTOR: PYRIDOXAL PHOSPHATE. Protein product from Mb0071c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0071c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:Q7U2X3" /db_xref="InterPro:IPR001085" /db_xref="InterPro:IPR015421" /db_xref="InterPro:IPR015422" /db_xref="InterPro:IPR015424" /db_xref="InterPro:IPR019798" /db_xref="InterPro:IPR039429" /db_xref="UniProtKB/Swiss-Prot:Q7U2X3" BEGIN 1 MNTLNDSLTA FDPDIAALID GELRRQESGL EMIASENYAP LAVMQAQGSV LTNKYAEGYP 61 GRRYYGGCEF VDGVEQLAID RVKALFGAEY ANVQPHSGAT ANAATMHALL NPGDTILGLS 121 LAHGGHLTHG MRINFSGKLY HATAYEVSKE DYLVDMDAVA EAARTHRPKM IIAGWSAYPR 181 QLDFARFRAI ADEVDAVLMV DMAHFAGLVA AGVHPSPVPH AHVVTSTTHK TLGGPRGGII 241 LCNDPAIAKK INSAVFPGQQ GGPLGHVIAA KATAFKMAAQ PEFAQRQQRC LDGARILAGR 301 LTQPDVAERG IAVLTGGTDV HLVLVDLRDA ELDGQQAEDR LAAVDITVNR NAVPFDPRPP 361 MITSGLRIGT PALAARGFSH NDFRAVADLI AAALTATNDD QLGPLRAQVQ RLAARYPLYP 421 ELHRT //