LOCUS       SIT98438.1               380 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            CELLULASE CELA1 (ENDOGLUCANASE) (ENDO-1,4-BETA-GLUCANASE)
            (FI-CMCASE) (CARBOXYMETHYL CELLULASE) protein.
ACCESSION   LT708304-63
PROTEIN_ID  SIT98438.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="celA1"
                     /locus_tag="BQ2027_MB0063"
                     /note="Mb0063, celA1, len: 380 aa. Equivalent to Rv0062,
                     len: 380 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.7% identity in 380 aa overlap). Possible celA1,
                     cellulase (EC 3.2.1.4), similar to many e.g.
                     AB65568.1|AL136058 putative secreted endoglucanase
                     (cellulase) from Streptomyces coelicolor (332 aa);
                     P07984|GUNA_CELFI ENDOGLUCANASE A PRECURSOR from
                     Cellulomonas fimi (449 aa); GUN1_STRHA|P33682
                     endoglucanase 1 precursor (cellulase) from STREPTOMYCES
                     HALSTEDII (321 aa), FASTA scores: opt: 702, E(): 1. 2e-27,
                     (38.9% identity in 319 aa overlap); etc. SEEMS TO BELONG
                     TO CELLULASE FAMILY B (FAMILY 6 OF GLYCOSYL HYDROLASES).
                     Note that previously known as celA. Protein product from
                     Mb0063 detected using shotgun mass spectrometry and SWATH
                     mass spectrometry. Mb0063 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:A0A1R3XU64"
                     /db_xref="InterPro:IPR016288"
                     /db_xref="InterPro:IPR036434"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3XU64"
BEGIN
        1 MTRRTGQRWR GTLPGRRPWT RPAPATCRRH LAFVELRHYF ARVMSSAIGS VARWIVPLLG
       61 VAAVASIGVI ADPVRVVRAP ALILVDAANP LAGKPFYVDP ASAAMIAARN ANPPNAELTS
      121 VANTPQSYWL DQAFPPATVG GTVARYTGAA QAAGAMPVLT LYGIPHRDCG SYASGGFATG
      181 TDYRGWIDAV ASGLGSSPAT IIVEPDALAM ADCLSPDQRQ ERFDLVRYAV DTLTRDPAAA
      241 VYVDAGHSRW LSAEAMAARL NDVGVGRARG FSLNVSNFYT TDEEIGYGEA ISGLTNGSHY
      301 VIDTSRNGAG PAPDAPLNWC NPSGRALGAP PTTATAGAHA DAYLWIKRPG ESDGTCGRGE
      361 PQAGRFVSQY AIDLAHNAGQ
//