LOCUS SIT98438.1 380 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE CELLULASE CELA1 (ENDOGLUCANASE) (ENDO-1,4-BETA-GLUCANASE) (FI-CMCASE) (CARBOXYMETHYL CELLULASE) protein. ACCESSION LT708304-63 PROTEIN_ID SIT98438.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="celA1" /locus_tag="BQ2027_MB0063" /note="Mb0063, celA1, len: 380 aa. Equivalent to Rv0062, len: 380 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 380 aa overlap). Possible celA1, cellulase (EC 3.2.1.4), similar to many e.g. AB65568.1|AL136058 putative secreted endoglucanase (cellulase) from Streptomyces coelicolor (332 aa); P07984|GUNA_CELFI ENDOGLUCANASE A PRECURSOR from Cellulomonas fimi (449 aa); GUN1_STRHA|P33682 endoglucanase 1 precursor (cellulase) from STREPTOMYCES HALSTEDII (321 aa), FASTA scores: opt: 702, E(): 1. 2e-27, (38.9% identity in 319 aa overlap); etc. SEEMS TO BELONG TO CELLULASE FAMILY B (FAMILY 6 OF GLYCOSYL HYDROLASES). Note that previously known as celA. Protein product from Mb0063 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0063 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XU64" /db_xref="InterPro:IPR016288" /db_xref="InterPro:IPR036434" /db_xref="UniProtKB/TrEMBL:A0A1R3XU64" BEGIN 1 MTRRTGQRWR GTLPGRRPWT RPAPATCRRH LAFVELRHYF ARVMSSAIGS VARWIVPLLG 61 VAAVASIGVI ADPVRVVRAP ALILVDAANP LAGKPFYVDP ASAAMIAARN ANPPNAELTS 121 VANTPQSYWL DQAFPPATVG GTVARYTGAA QAAGAMPVLT LYGIPHRDCG SYASGGFATG 181 TDYRGWIDAV ASGLGSSPAT IIVEPDALAM ADCLSPDQRQ ERFDLVRYAV DTLTRDPAAA 241 VYVDAGHSRW LSAEAMAARL NDVGVGRARG FSLNVSNFYT TDEEIGYGEA ISGLTNGSHY 301 VIDTSRNGAG PAPDAPLNWC NPSGRALGAP PTTATAGAHA DAYLWIKRPG ESDGTCGRGE 361 PQAGRFVSQY AIDLAHNAGQ //