LOCUS SIT98432.1 874 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
REPLICATIVE DNA HELICASE DNAB protein.
ACCESSION LT708304-59
PROTEIN_ID SIT98432.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="dnaB"
/locus_tag="BQ2027_MB0059"
/note="Mb0059, dnaB, len: 874 aa. Equivalent to Rv0058,
len: 874 aa, from Mycobacterium tuberculosis strain H37Rv,
(99.9% identity in 874 aa overlap). Probable dnaB,
replicative DNA helicase (EC 3.6.1.-). Contains an intein
(position 61630..62838) similar to, and in the same
position as, those in Sycnechocystis and Rhodothermus
marinus (see citation below). Highly similar to others
e.g. DNAB_SYNY3|Q55418 replicative dna helicase (872 aa),
FASTA scores: opt: 533, E(): 0, (32.5% identity in 424 aa
overlap). Also similar to intein recA|E1173867|AL008967
RECA INTEIN from Mycobacterium tuberculosis (442 aa),
FASTA scores: E(): 3.8e-16, (27.0% identity in 426 aa
overlap). C-terminal extein (position 62839..63015)
similar to many dnaB proteins e.g.
NP_302709.1|NC_002677|P46394|DNAB_MYCLE REPLICATIVE DNA
HELICASE from Mycobacterium leprae (604 aa);
DNAB_ECOLI|P03005 replicative dna helicase from
Escherichia coli (471 aa), FASTA scores: opt: 148, E():
1.5e-07, (37.9% identity in 58 aa overlap); etc. THIS
PROTEIN UNDERGOES A PROTEIN SELF SPLICING THAT INVOLVES A
POST-TRANSLATIONAL EXCISION OF THE INTERVENING REGION
(INTEIN) FOLLOWED BY PEPTIDE LIGATION. BELONGS TO THE
HELICASE FAMILY, DNAB SUBFAMILY. IN THE INTEIN SECTION;
BELONGS TO THE HOMING ENDONUCLEASE FAMILY. Mb0059 found to
be expressed during exponential growth in Sauton's minimal
media by RNA-sequencing (TPM > 20)."
/db_xref="GOA:P59966"
/db_xref="InterPro:IPR003586"
/db_xref="InterPro:IPR003587"
/db_xref="InterPro:IPR003593"
/db_xref="InterPro:IPR004042"
/db_xref="InterPro:IPR004860"
/db_xref="InterPro:IPR006141"
/db_xref="InterPro:IPR006142"
/db_xref="InterPro:IPR007692"
/db_xref="InterPro:IPR007693"
/db_xref="InterPro:IPR007694"
/db_xref="InterPro:IPR016136"
/db_xref="InterPro:IPR027417"
/db_xref="InterPro:IPR027434"
/db_xref="InterPro:IPR030934"
/db_xref="InterPro:IPR036185"
/db_xref="InterPro:IPR036844"
/db_xref="UniProtKB/Swiss-Prot:P59966"
BEGIN
1 MAVVDDLAPG MDSSPPSEDY GRQPPQDLAA EQSVLGGMLL SKDAIADVLE RLRPGDFYRP
61 AHQNVYDAIL DLYGRGEPAD AVTVAAELDR RGLLRRIGGA PYLHTLISTV PTAANAGYYA
121 SIVAEKALLR RLVEAGTRVV QYGYAGAEGA DVAEVVDRAQ AEIYDVADRR LSEDFVALED
181 LLQPTMDEID AIASSGGLAR GVATGFTELD EVTNGLHPGQ MVIVAARPGV GKSTLGLDFM
241 RSCSIRHRMA SVIFSLEMSK SEIVMRLLSA EAKIKLSDMR SGRMSDDDWT RLARRMSEIS
301 EAPLFIDDSP NLTMMEIRAK ARRLRQKANL KLIVVDYLQL MTSGKKYESR QVEVSEFSRH
361 LKLLAKELEV PVVAISQLNR GPEQRTDKKP MLADLRESGC LTASTRILRA DTGAEVAFGE
421 LMRSGERPMV WSLDERLRMV ARPMINVFPS GRKEVFRLRL ASGREVEATG SHPFMKFEGW
481 TPLAQLKVGD RIAAPRRVPE PIDTQRMPES ELISLARMIG DGSCLKNQPI RYEPVDEANL
541 AAVTVSAAHS DGAAIRDDYL AARVPSLRPA RQRLPRGRCT PIAAWLAGLG LFTKRSHEKC
601 VPEAVFRAPN DQVALFLRHL WSAGGSVRWD PTNGQGRVYY GSTSRRLIDD VAQLLLRVGI
661 FSWITHAPKL GGHDSWRLHI HGAKDQVRFL RHVGVHGAEA VAAQEMLRQL KGPVRNPNLD
721 SAPKKVWAQV RNRLSAKQMM DIQLHEPTMW KHSPSRSRPH RAEARIEDRA IHELARGDAY
781 WDTVVEITSI GDQHVFDGTV SGTHNFVANG ISLHNSLEQD ADVVILLHRP DAFDRDDPRG
841 GEADFILAKH RNGPTKTVTV AHQLHLSRFA NMAR
//