LOCUS SIT98418.1 187 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Intracellular protease protein. ACCESSION LT708304-53 PROTEIN_ID SIT98418.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0053" /note="Mb0053, -, len: 187 aa. Equivalent to Rv0052, len: 187 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 187 aa overlap). Conserved hypothetical protein, similar to others e.g. AL049587|SC5F2A_30S|T35272 hypothetical protein from Streptomyces coelicolor (211 aa), FASTA scores: opt: 531, E(): 3.4e-29, (49.5% identity in 182 aa overlap); NP_420588.1|NC_002696 ThiJ/PfpI family protein from Caulobacter crescentus (267 aa); etc. Some similarity to Escherichia coli G1100872|thiJ (198 aa), FASTA scores: opt: 178, E(): 6.1e-06, (29.9% identity in 137 aa overlap). Also similar to Rv1930c from Mycobacterium tuberculosis (174 aa). May be a membrane protein. Protein product from Mb0053 detected using shotgun mass spectrometry. Mb0053 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR002818" /db_xref="InterPro:IPR029062" /db_xref="UniProtKB/TrEMBL:A0A1R3XU47" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPSFDVVFVG HRRGEVRSDN AMLGLLCDAA FDELTRPDVV IFPGGIGTRT LIHDQTVLDW 61 VREAHRHTLL TTSVCTGGLV LAAAGLLNGL TATTHWRVQD LFNSLGARYV PQRVVEHLPE 121 RVITAAGVSS GIDMGLRLVE LLVSREAAEA SQLMIEYDPQ PPVDAGSLAK ASPATHRLAL 181 EFYQHRL //