LOCUS SIT98407.1 367 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 MYO- INOSITOL-1-PHOSPHATE SYNTHASE INO1 (Inositol 1-phosphate synthetase) (D-glucose 6-phosphate cycloaldolase) (Glucose 6-phosphate cyclase) (Glucocycloaldolase) protein. ACCESSION LT708304-47 PROTEIN_ID SIT98407.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ino1" /locus_tag="BQ2027_MB0047C" /standard_name="tbINO" /note="Mb0047c, ino1, len: 367 aa. Equivalent to Rv0046c, len: 367 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 367 aa overlap). ino1 (alternate gene name: tbINO), myo-inositol-1-phosphate synthase (EC 5.5.1.4) (see citations below), equivalent to Q57240|Y046_MYCLE|U00015_14|G466956|B1620_F3_113 HYPOTHETICAL 40.3 KDA PROTEIN from Mycobacterium leprae (369 aa), FASTA scores: opt: 2221, E(): 0, (91.8% identity in 366 aa overlap). N-terminus similar to N-terminus of myo-inositol-1-phosphate synthases e.g. INO1_SPIPO|P42803 myo-inositol-1-phosphate synthase (510 aa), FASTA scores: opt: 144, E(): 0.021, (25.2% identity in 365 aa overlap); CAC21218.1|AJ401007 myo-inositol 1P synthase from Thermotoga sp. SG1 (335 aa); etc. Also highly similar to other hypothetical proteins e.g. AL049826|SCH24_21c hypothetical protein from Streptomyces coelicolor (360 aa), FASTA scores: opt: 1790, E(): 0, (77.8% identity in 360 aa overlap); AE000881_1 conserved protein from M. thermoautotrophicus (368 aa); etc. Protein product from Mb0047c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0047c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P59967" /db_xref="InterPro:IPR002587" /db_xref="InterPro:IPR013021" /db_xref="InterPro:IPR017815" /db_xref="InterPro:IPR036291" /db_xref="UniProtKB/Swiss-Prot:P59967" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSEHQSLPAP EASTEVRVAI VGVGNCASSL VQGVEYYYNA DDTSTVPGLM HVRFGPYHVR 61 DVKFVAAFDV DAKKVGFDLS DAIFASENNT IKIADVAPTN VIVQRGPTLD GIGKYYADTI 121 ELSDAEPVDV VQALKEAKVD VLVSYLPVGS EEADKFYAQC AIDAGVAFVN ALPVFIASDP 181 VWAKKFTDAG VPIVGDDIKS QVGATITHRV LAKLFEDRGV QLDRTMQLNV GGNMDFLNML 241 ERERLESKKI SKTQAVTSNL KREFKTKDVH IGPSDHVGWL DDRKWAYVRL EGRAFGDVPL 301 NLEYKLEVWD SPNSAGVIID AVRAAKIAKD RGIGGPVIPA SAYLMKSPPE QLPDDIARAQ 361 LEEFIIG //