LOCUS SIT98377.1 477 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CONSERVED HYPOTHETICAL PROTEIN protein. ACCESSION LT708304-27 PROTEIN_ID SIT98377.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0027" /note="Mb0027, -, len: 477 aa. Equivalent to 5' end of Rv0026, len: 448 aa, from Mycobacterium tuberculosis strain H37Rv, (94.4% identity in 449 aa overlap). Conserved hypothetical protein, showing some similarity to other proteins from Mycobacterium tuberculosis: Rv0025 FASTA score: (35.4% identity in 113 aa overlap) and Rv0739 (268 aa), FASTA score: (32.4% identity in 142 aa overlap). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a 4 bp insertion (*-atcg) leads to a longer protein with a different COOH part compared to its homolog in Mycobacterium tuberculosis strain H37Rv (477 aa versus 448 aa). Mb0027 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="InterPro:IPR019710" /db_xref="UniProtKB/TrEMBL:A0A1R3XU28" BEGIN 1 MAFDAAMSTH EDLLATIRYV RDRTGDPNAW QTGLTPTEVT AVVTSTTRSE QLDAILRKIR 61 QRHSNLYYPA PPDREQGDAA RAIADAEAAL AHPNSATAQL DLQVVSAILN AHLKTVEGGE 121 SLHELQQEIE AAVRIRSDLD TPAGARDFQR FLIGKLKDIR EVVATASLDA ASKSALMAAW 181 TSLYDASKGD RGDADDRGPA SVGSGGAPAR GAGQQPELPT RAEPDCLLDS LLLEDPGLLA 241 DDLQVPGGTS AAIPSASSTP SLPNLGGATM PGGGATPALV PGVSAPGGLP LSGLLRGVGD 301 EPELTDFDER GQEVRDPADY EHSNEPDERR ADDREGADED AGLGKSESPP QAPTTVTLPN 361 GETVTAASPQ LAAAIKAAAS GTPIADAFQQ QGIAIPLPGT AVANPVDPAR ISAGDVGVFT 421 DRHALALGPS KALLDGQIQH ISAVRGRNFL GWIHPAATAT APARTEAPTP TRPAAAR //