LOCUS SIT98373.1 277 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PUTATIVE SECRETED PROTEIN P60-RELATED PROTEIN [FIRST PART] protein. ACCESSION LT708304-24 PROTEIN_ID SIT98373.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0024" /note="Mb0024, -, len: 277 aa. Equivalent to 5' end of Rv0024, len: 281 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 192 aa overlap). Putative secreted protein, p60 homologue, similar in part to others and relatives proteins e.g. P60_LISIV|Q01837 protein p60 precursor (invasion-associated protein) from Listeria ivanovii (524 aa), FASTA scores: opt: 245, E(): 1.5e-08, (37.0% identity in 100 aa overlap); CAB92656.1|AL356832 putative NPL/P60 family secreted protein from Streptomyces coelicolor (347 aa); etc. Similar to Mycobacterium tuberculosis proteins Rv1477, Rv1478, Rv1566c, Rv2190c. And several homologues in Streptomyces coelicolor e.g. AL049497|SC6G10_8|T35517 probable secreted protein (338 aa), FASTA scores: opt: 399, E(): 9.8e-18, (34.9% identity in 292 aa overlap). COULD BELONG TO THE E. COLI NLPC / LISTERIA P60 FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv0024 exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base deletion (c-*) splits Rv0024 into 2 parts, Mb0024 and Mb0025. Mb0024 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="UniProtKB/TrEMBL:A0A1R3XU42" BEGIN 1 MNYSEVELLS RAHQLFAGDS RRPGLDAGTT PYGDLLSRAA DLNVGAGQRR YQLAVDHSRA 61 ALLSAARTDA AAGAVITGAQ RDRAWARRST GTVLDEARSD TTVTAVMPIA QREAIRRRVA 121 RLRAQRAHVL TARRRARRHL AALRALRYRV AHGPGVALAK LRLPSPSGRA GIAVHAALSR 181 LGRPYVWGAT GPTSSTVPVW SSGPTPRRVF TWIAPPINRS TRGSRCRAHR SGRAIWSSRT 241 PGTCSWRSAT IWSSRRPMRA RRFGSARWAT TCRFGDR //