LOCUS SIT98371.1 256 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN protein. ACCESSION LT708304-23 PROTEIN_ID SIT98371.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0023" /note="Mb0023, -, len: 256 aa. Equivalent to Rv0023, len: 256 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 256 aa overlap). Possible transcriptional regulator, equivalent to CAB96432.1|AJ251434 hypothetical protein from Mycobacterium avium subsp. paratuberculosis (146 aa). N-terminus showing similarity with other transcriptional regulators e.g. AE0002|ECAE000240_9 from Escherichia coli strain K12 (178 aa), FASTA scores: opt: 149, E(): 0.0048, (33.3% identity in 84 aa overlap); etc. Contains probable helix-turn helix motif from aa 19 to 40 (Score 1615, +4.69 SD). Protein product from Mb0023 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0023 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /db_xref="GOA:P67705" /db_xref="InterPro:IPR001387" /db_xref="InterPro:IPR010982" /db_xref="UniProtKB/Swiss-Prot:P67705" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MSRESAGAAI RALRESRDWS LADLAAATGV STMGLSYLER GARKPHKSTV QKVENGLGLP 61 PGTYSRLLVA ADPDAELARL IAAQPSNPTA VRRAGAVVVD RHSDTDVLEG YAEAQLDAIK 121 SVIDRLPATT SNEYETYILS VIAQCVKAEM LAASSWRVAV NAGADSTGRL MEHLRALEAT 181 RGALLERMPT SLSARFDRAC AQSSLPEAVV AALIGVGADE MWDIRNRGVI PAGALPRVRA 241 FVDAIEASHD ADEGQQ //