LOCUS SIT98363.1 514 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 phosphoserine/ threonine phosphatase pstp protein. ACCESSION LT708304-18 PROTEIN_ID SIT98363.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pstp" /locus_tag="BQ2027_MB0018C" /note="Mb0018c, ppp, len: 514 aa. Equivalent to Rv0018c, len: 514 aa, from Mycobacterium tuberculosis strain H37Rv, (99.4% identity in 514 aa overlap). Possible ppp, serine/threonine phosphatase (EC 3.1.3.16), equivalent to MLCB1770_13|T10013 PUTATIVE PHOSPHOPROTEIN PHOSPHATASE from Mycobacterium leprae (509 aa), FASTA scores: opt: 2517, E(): 0. Also highly similar to others e.g. T36714 probable protein phosphatase from Streptomyces coelicolor (515 aa); CAA10712.1|AJ132604 pppL protein from Lactococcus lactis (258 aa); NP_248765.1|NC_002516 probable phosphoprotein phosphatase from Pseudomonas aeruginosa (242 aa); etc. Also similar to BSUB0009_46 YLOO PROTEIN from Bacillus subtilis (254 aa), FASTA score: (34.0% identity in 250 aa overlap). Protein product from Mb0018c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0018c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XV75" /db_xref="InterPro:IPR001932" /db_xref="InterPro:IPR015655" /db_xref="InterPro:IPR036457" /db_xref="UniProtKB/TrEMBL:A0A1R3XV75" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MALVTLVLRY AARSDRGLVR ANNEDSVYAG ARLLALADGM GGHAAGEVAS QLVIAALAHL 61 DDDEPGGDLL AKLDAAVRAG NSAIAAQVEM EPDLEGMGTT LTAILFAGNR LGLVHIGDSR 121 GYLLRDGELT QITKDDTFVQ TLVDEGRITP EEAHSHPQRS LIMRALTGHE VEPTLTMREA 181 RAGDRYLLCS DGLSDPVSDE TILEALQIPE VAESAHRLIE LALRGGGPDN VTVVVADVVD 241 YDYGQTQPIL AGAVSGDDDQ LTLPNTAAGR ASAISQRKEI VKRVPPQADT FSRPRWSGRR 301 LAFVVALVTV LMTAGLLIGR AIIRSNYYVA DYAGSVSIMR GIQGSLLGMS LHQPYLMGCL 361 SPRNELSQIS YGQSGGPLDC HLMKLEDLRP PERAQVRAGL PAGTLDDAIG QLRELAANSL 421 LPPCPAPRAT SPPGRPAPPT TSETTEPNVT SSPAAPSPTT SASAPTGTTP AIPTSASPAA 481 PASPPTPWPV TSSPTMAALP PPPPQPGIDC RAAA //