LOCUS SIT98361.1 491 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PENICILLIN-BINDING PROTEIN PBPA protein. ACCESSION LT708304-16 PROTEIN_ID SIT98361.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pbpA" /locus_tag="BQ2027_MB0016C" /note="Mb0016c, pbpA, len: 491 aa. Equivalent to Rv0016c, len: 491 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 491 aa overlap). Probable pbpA, penicillin-binding protein, equivalent to NP_301144.1|NC_002677 putative penicillin-binding protein from Mycobacterium leprae (492 aa); and highly similar to MLCB1770_1 penicillin binding protein from Mycobacterium leprae (474 aa), FASTA scores: opt: 2516, E(): 0, (82.4% identity in 472 aa overlap). Also similar to others e.g. T36716 from Streptomyces coelicolor (490 aa); AAF61246.1|AF241575|PbpA from Streptomyces griseus (485 aa); NP_347146.1|NC_003030 from Clostridium acetobutylicum (482 aa); E235825|pbpA penicillin binding protein (325 aa), FASTA scores: opt: 1618, E(): 0, (78.3% identity in 323 aa overlap); etc. And also similar to MTCY270_5 and MTV003_8 from Mycobacterium tuberculosis. Protein product from Mb0016c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0016c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XU21" /db_xref="InterPro:IPR001460" /db_xref="InterPro:IPR012338" /db_xref="UniProtKB/TrEMBL:A0A1R3XU21" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MNASLRRISV TVMALIVLLL LNATMTQVFT ADGLRADPRN QRVLLDEYSR QRGQITAGGQ 61 LLAYSVATDG RFRFLRVYPN PEVYAPVTGF YSLRYSSTAL ERAEDPILNG SDRRLFGRRL 121 ADFFTGRDPR GGNVDTTINP RIQQAGWDAM QQGCYGPCKG AVVALEPSTG KILALVSSPS 181 YDPNLLASHN PEVQAQAWQR LGDNPASPLT NRAISETYPP GSTFKVITTA AALAAGATET 241 EQLTAAPTIP LPGSTAQLEN YGGAPCGDEP TVSLREAFVK SCNTAFVQLG IRTGADALRS 301 MARAFGLDSP PRPTPLQVAE STVGPIPDSA ALGMTSIGQK DVALTPLANA EIAATIANGG 361 ITMRPYLVGS LKGPDLANIS TTVGYQQRRA VSPQVAAKLT ELMVGAEKVA QQKGAIPGVQ 421 IASKTGTAEH GTDPRHTPPH AWYIAFAPAQ APKVAVAVLV ENGADRLSAT GGALAAPIGR 481 AVIEAALQGE P //