LOCUS       SIT98360.1               431 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 TRANSMEMBRANE
            SERINE/THREONINE-PROTEIN KINASE A PKNA (PROTEIN KINASE
            A) (STPK A) protein.
ACCESSION   LT708304-15
PROTEIN_ID  SIT98360.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="pknA"
                     /locus_tag="BQ2027_MB0015C"
                     /note="Mb0015c, pknA, len: 431 aa. Equivalent to Rv0015c,
                     len: 431 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 431 aa overlap). pknA, transmembrane
                     serine/threonine-protein kinase (EC 2.7.1.-),
                     magnesium/manganese dependent (see citations below),
                     equivalent to MLCB1770_10|NP_301143.1|NC_002677 putative
                     serine/threonine protein kinase from Mycobacterium leprae
                     (437 aa), FASTA scores: opt: 1883, E(): 0, (72.1% identity
                     in 434 aa overlap). And also highly similar to other
                     kinases from Mycobacterium leprae e.g. MLCB1770_10 from
                     Mycobacterium leprae (437 aa). Also similar to PKNA_MYCLE
                     protein kinase (253 aa), FASTA scores: opt: 1525, E(): 0,
                     (95.0% identity in 242 aa overlap); etc. Also highly
                     similar in part to others e.g. N-terminus of
                     NP_243370.1|NC_002570 from Bacillus halodurans (664 aa);
                     N-terminus of T36717 from Streptomyces coelicolor (673
                     aa); etc. Also similar to others from Mycobacterium
                     tuberculosis: MTCY10H4_15, MTV021_9, MTCY28_5,
                     MTCY4C12_28, MTCY50_16, MTCY8D9_8, MTCY49_28, MTCY4C12_30,
                     MTCY28_9, etc. Contains PS00108 Serine/Threonine protein
                     kinases active-site signature. Contains Hank's kinase
                     subdomain. BELONGS TO THE SER/THR FAMILY OF PROTEIN
                     KINASES. It has been shown that sodium orthovanadate
                     inhibits the activity of the enzyme in vitro. Protein
                     product from Mb0015c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0015c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:P65727"
                     /db_xref="InterPro:IPR000719"
                     /db_xref="InterPro:IPR008271"
                     /db_xref="InterPro:IPR011009"
                     /db_xref="UniProtKB/Swiss-Prot:P65727"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MSPRVGVTLS GRYRLQRLIA TGGMGQVWEA VDNRLGRRVA VKVLKSEFSS DPEFIERFRA
       61 EARTTAMLNH PGIASVHDYG ESQMNGEGRT AYLVMELVNG EPLNSVLKRT GRLSLRHALD
      121 MLEQTGRALQ IAHAAGLVHR DVKPGNILIT PTGQVKITDF GIAKAVDAAP VTQTGMVMGT
      181 AQYIAPEQAL GHDASPASDV YSLGVVGYEA VSGKRPFAGD GALTVAMKHI KEPPPPLPPD
      241 LPPNVRELIE ITLVKNPAMR YRSGGPFADA VAAVRAGRRP PRPSQTPPPG RAAPAAIPSG
      301 TTARVAANSA GRTAASRRSR PATGGHRPPR RTFSSGQRAL LWAAGVLGAL AIIIAVLLVI
      361 KAPGDNSPQQ APTPTVTTTG NPPASNTGGT DASPRLNWTE RGETRHSGLQ SWVVPPTPHS
      421 RASLARYEIA Q
//