LOCUS SIT98357.1 262 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED MEMBRANE PROTEIN protein. ACCESSION LT708304-12 PROTEIN_ID SIT98357.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0012" /note="Mb0012, -, len: 262 aa. Equivalent to Rv0012, len: 262 aa, from Mycobacterium tuberculosis strain H37Rv (99.2% identity in 262 aa overlap). Probable conserved membrane protein, similar to AL079308|SCH69_23|T36722 hypothetical protein from Streptomyces coelicolor (237 aa), FASTA scores: opt: 506, E(): 1.9e-25, (39.8% identity in 236 aa overlap). Some similarity to BLU0|1958_35A2 DIVIB (fragment) (188 aa), FASTA scores: opt: 204, E(): 8.9e-07, (35.6% identity in 90 aa overlap); and G1129091|DDS cell division and sporulation protein from Bacillus subtilis (231 aa), FASTA scores: opt: 180, E(): 3.8e-05, (30.7% identity in 101 aa overlap). Also similar to Rv1823|MTCY1A11_20 from Mycobacterium tuberculosis FASTA score: (30.1% identity in 246 aa overlap); and MTCY1A11_18 FASTA score: (25.5% identity in 235 aa overlap). Contains probable N-terminal signal sequence. Protein product from Mb0012 detected using SWATH mass spectrometry. Mb0012 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="InterPro:IPR010273" /db_xref="UniProtKB/TrEMBL:A0A1R3XU20" BEGIN 1 MRLTHPTPCP ENGETMIDRR RSAWRFSVPL VCLLAGLLLA ATHGVSGGTE IRRSDAPRLV 61 DLVRRAQASV NRLATEREAL TTRIDSVHGR SVDTALAAMQ RRSAELAGVA AMNPVHGPGL 121 VVTLQDAQRD ANGRFPRDAS PDDLVVHQQD IEAVLNALWN AGAEAIQMQD QRIIAMSIAR 181 CVGNTLLLNG RTYSPPYTIA AIGDAAAMQA ALAAAPLVTL YKQYVVRFGL GYREEVHPDL 241 QIVGYADPVR MHFAQPAGPL DY //