LOCUS SIT98343.1 714 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 DNA GYRASE (SUBUNIT B) GYRB (DNA TOPOISOMERASE (ATP-HYDROLYSING) ) (DNA TOPOISOMERASE II) (TYPE II DNA TOPOISOMERASE) protein. ACCESSION LT708304-5 PROTEIN_ID SIT98343.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="gyrB" /locus_tag="BQ2027_MB0005" /note="Mb0005, gyrB, len: 714 aa. Equivalent to Rv0005, len: 714 aa, from Mycobacterium tuberculosis strain H37Rv, (99.9% identity in 714 aa overlap). gyrB, DNA gyrase subunit B (EC 5.99.1.3) (see citations below), equivalent, except in N-terminus, to other Mycobacterial DNA GYRASES SUBUNIT B e.g. T10005 from Mycobacterium leprae (697 aa); Q9L7L3|GYRB_MYCPA from Mycobacterium avium subsp. paratuberculosis (677 aa) (has its N-terminus shorter); P48355|GYRB_MYCSM from Mycobacterium smegmatis (675 aa); etc. Also highly similar to others e.g. T10969 from Streptomyces coelicolor (686 aa); P50075|GYBS_STRSH from Streptomyces spheroides (684 aa); etc. Contains PS00177 DNA topoisomerase II signature. BELONGS TO THE TYPE II TOPOISOMERASE FAMILY. Protein product from Mb0005 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0005 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:A0A1R3XU15" /db_xref="InterPro:IPR001241" /db_xref="InterPro:IPR002288" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR006171" /db_xref="InterPro:IPR011557" /db_xref="InterPro:IPR013506" /db_xref="InterPro:IPR013759" /db_xref="InterPro:IPR013760" /db_xref="InterPro:IPR014721" /db_xref="InterPro:IPR018522" /db_xref="InterPro:IPR020568" /db_xref="InterPro:IPR034160" /db_xref="InterPro:IPR036890" /db_xref="UniProtKB/TrEMBL:A0A1R3XU15" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MGKNEARRSA LAPDHGTVVC DPLRRLNRMH ATPEESIRIV AAQKKKAQDE YGAASITILE 61 GLEAVRKRPG MYIGSTGERG LHHLIWEVVD NAVDEAMAGY ATTVNVVLLE DGGVEVADDG 121 RGIPVATHAS GIPTVDVVMT QLHAGGKFDS DAYAISGGLH GVGVSVVNAL STRLEVEIKR 181 DGYEWSQVYE KSEPLGLKQG APTKKTGSTV RFWADPAVFE TTEYDFETVA RRLQEMAFLN 241 KGLTINLTDE RVTQDEVVDE VVSDVAEAPK SASERAAEST APHKVKSRTF HYPGGLVDFV 301 KHINRTKNAI HSSIVDFSGK GTGHEVEIAM QWNAGYSESV HTFANTINTH EGGTHEEGFR 361 SALTSVVNKY AKDRKLLKDK DPNLTGDDIR EGLAAVISVK VSEPQFEGQT KTKLGNTEVK 421 SFVQKVCNEQ LTHWFEANPT DSKVVVNKAV SSAQARIAAR KARELVRRKS ATDIGGLPGK 481 LADCRSTDPR KSELYVVEGD SAGGSAKSGR DSMFQAILPL RGKIINVEKA RIDRVLKNTE 541 VQAIITALGT GIHDEFDIGK LRYHKIVLMA DADVDGQHIS TLLLTLLFRF MRPLIENGHV 601 FLAQPPLYKL KWQRSDPEFA YSDRERDGLL EAGLKAGKKI NKEDGIQRYK GLGEMDAKEL 661 WETTMDPSVR VLRQVTLDDA AAADELFSIL MGEDVDARRS FITRNAKDVR FLDV //