LOCUS SIT98337.1 402 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 DNA POLYMERASE III (BETA CHAIN) DNAN (DNA NUCLEOTIDYLTRANSFERASE) protein. ACCESSION LT708304-2 PROTEIN_ID SIT98337.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dnaN" /locus_tag="BQ2027_MB0002" /note="Mb0002, dnaN, len: 402 aa. Equivalent to Rv0002, len: 402 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 402 aa overlap). dnaN, DNA polymerase III (beta chain) (EC 2.7.7.7) (see citations below), equivalent to other Mycobacterial DNA POLYMERASES III BETA CHAIN e.g. NP_301130.1|NC_002677 from Mycobacterium leprae (399 aa); Q9L7L6|DP3B_MYCPA from Mycobacterium avium subsp. paratuberculosis (399 aa); P52851|DP3B_MYCSM from Mycobacterium smegmatis (397 aa); etc. Also highly similar to others e.g. P27903|DP3B_STRCO DNA POLYMERASE III BETA CHAIN from Streptomyces coelicolor (376 aa), FASTA scores: opt: 1189, E(): 0, (52.8% identity in 337 aa overlap); P21174|DP3B_MICLU from Micrococcus luteus (310 aa); P52023|DP3B_SYNP7 from Synechococcus sp. strain PCC 7942 (375 aa); etc. Overlaps and extends CDS in neighbouring cosmid MTCY10H4.01. Protein product from Mb0002 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0002 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:O33914" /db_xref="InterPro:IPR001001" /db_xref="InterPro:IPR022634" /db_xref="InterPro:IPR022635" /db_xref="InterPro:IPR022637" /db_xref="UniProtKB/Swiss-Prot:O33914" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD 61 YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVGV HVEGNRVALT CGNARFSLPT 121 MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ILGETVVLAA 181 TDRFRLAVRE LKWSASSPDI EAAVLVPAKT LAEAAKAGIG GSDVRLSLGT GPGVGKDGLL 241 GISGNGKRST TRLLDAEFPK FRQLLPTEHT AVATMDVAEL IEAIKLVALV ADRGAQVRME 301 FADGSVRLSA GADDVGRAEE DLVVDYAGEP LTIAFNPTYL TDGLSSLRSE RVSFGFTTAG 361 KPALLRPVSG DDRPVAGLNG NGPFPAVSTD YVYLLMPVRL PG //