LOCUS       SIT98337.1               402 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 DNA
            POLYMERASE III (BETA CHAIN) DNAN (DNA NUCLEOTIDYLTRANSFERASE)
            protein.
ACCESSION   LT708304-2
PROTEIN_ID  SIT98337.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="dnaN"
                     /locus_tag="BQ2027_MB0002"
                     /note="Mb0002, dnaN, len: 402 aa. Equivalent to Rv0002,
                     len: 402 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (99.8% identity in 402 aa overlap). dnaN, DNA polymerase
                     III (beta chain) (EC 2.7.7.7) (see citations below),
                     equivalent to other Mycobacterial DNA POLYMERASES III BETA
                     CHAIN e.g. NP_301130.1|NC_002677 from Mycobacterium leprae
                     (399 aa); Q9L7L6|DP3B_MYCPA from Mycobacterium avium
                     subsp. paratuberculosis (399 aa); P52851|DP3B_MYCSM from
                     Mycobacterium smegmatis (397 aa); etc. Also highly similar
                     to others e.g. P27903|DP3B_STRCO DNA POLYMERASE III BETA
                     CHAIN from Streptomyces coelicolor (376 aa), FASTA scores:
                     opt: 1189, E(): 0, (52.8% identity in 337 aa overlap);
                     P21174|DP3B_MICLU from Micrococcus luteus (310 aa);
                     P52023|DP3B_SYNP7 from Synechococcus sp. strain PCC 7942
                     (375 aa); etc. Overlaps and extends CDS in neighbouring
                     cosmid MTCY10H4.01. Protein product from Mb0002 detected
                     using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb0002 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:O33914"
                     /db_xref="InterPro:IPR001001"
                     /db_xref="InterPro:IPR022634"
                     /db_xref="InterPro:IPR022635"
                     /db_xref="InterPro:IPR022637"
                     /db_xref="UniProtKB/Swiss-Prot:O33914"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MDAATTRVGL TDLTFRLLRE SFADAVSWVA KNLPARPAVP VLSGVLLTGS DNGLTISGFD
       61 YEVSAEAQVG AEIVSPGSVL VSGRLLSDIT RALPNKPVGV HVEGNRVALT CGNARFSLPT
      121 MPVEDYPTLP TLPEETGLLP AELFAEAISQ VAIAAGRDDT LPMLTGIRVE ILGETVVLAA
      181 TDRFRLAVRE LKWSASSPDI EAAVLVPAKT LAEAAKAGIG GSDVRLSLGT GPGVGKDGLL
      241 GISGNGKRST TRLLDAEFPK FRQLLPTEHT AVATMDVAEL IEAIKLVALV ADRGAQVRME
      301 FADGSVRLSA GADDVGRAEE DLVVDYAGEP LTIAFNPTYL TDGLSSLRSE RVSFGFTTAG
      361 KPALLRPVSG DDRPVAGLNG NGPFPAVSTD YVYLLMPVRL PG
//