LOCUS SIT98333.1 507 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 CHROMOSOMAL REPLICATION INITIATOR PROTEIN DNAA protein. ACCESSION LT708304-1 PROTEIN_ID SIT98333.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="dnaA" /locus_tag="BQ2027_MB0001" /note="Mb0001, dnaA, len: 507 aa. Equivalent to Rv0001, len: 507 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 507 aa overlap). dnaA, chromosomal replication initiator protein (see citations below), equivalent to other Mycobacterial CHROMOSOMAL REPLICATION INITIATOR PROTEINS e.g. P46388|DNAA_MYCLE from Mycobacterium leprae (502 aa); Q9L7L7|DNAA_MYCPA from Mycobacterium paratuberculosis (509 aa); P49990|DNAA_MYCAV from Mycobacterium avium (508 aa); P49992|DNAA_MYCSM from Mycobacterium smegmatis (504 aa); etc. Also highly similar to others except in N-terminus e.g. Q9ZH75|DNAA_STRCH CHROMOSOMAL REPLICATION INITIATOR PROTEIN from Streptomyces chrysomallus (624 aa); Q9ZH76|DNAA_STRRE from Streptomyces reticuli (643 aa); DNAA_ECOLI|P03004|B3702 chromosomal replication initiator protein from Escherichia coli strain K12 (467 aa), FASTA scores: opt: 986, E(): 0, (43.2% identity in 389 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop) and PS01008 DnaA protein signature. BELONGS TO THE DNAA FAMILY. Note that the first base of this gene has been taken as base 1 of the Mycobacterium bovis genomic sequence. Protein product from Mb0001 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0001 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /db_xref="GOA:P49991" /db_xref="InterPro:IPR001957" /db_xref="InterPro:IPR003593" /db_xref="InterPro:IPR010921" /db_xref="InterPro:IPR013159" /db_xref="InterPro:IPR013317" /db_xref="InterPro:IPR018312" /db_xref="InterPro:IPR020591" /db_xref="InterPro:IPR027417" /db_xref="UniProtKB/Swiss-Prot:P49991" /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTDDPGSGFT TVWNAVVSEL NGDPKVDDGP SSDANLSAPL TPQQRAWLNL VQPLTIVEGF 61 ALLSVPSSFV QNEIERHLRA PITDALSRRL GHQIQLGVRI APPATDEADD TTVPPSENPA 121 TTSPDTTTDN DEIDDSAAAR GDNQHSWPSY FTERPRNTDS ATAGVTSLNR RYTFDTFVIG 181 ASNRFAHAAA LAIAEAPARA YNPLFIWGES GLGKTHLLHA AGNYAQRLFP GMRVKYVSTE 241 EFTNDFINSL RDDRKVAFKR SYRDVDVLLV DDIQFIEGKE GIQEEFFHTF NTLHNANKQI 301 VISSDRPPKQ LATLEDRLRT RFEWGLITDV QPPELETRIA ILRKKAQMER LAIPDDVLEL 361 IASSIERNIR ELEGALIRVT AFASLNKTPI DKALAEIVLR DLIADANTMQ ISAATIMAAT 421 AEYFDTTVEE LRGPGKTRAL AQSRQIAMYL CRELTDLSLP KIGQAFGRDH TTVMYAQRKI 481 LSEMAERREV FDHVKELTTR IRQRSKR //