LOCUS QCY27719.1 403 aa PRT BCT 06-JUN-2019 DEFINITION Priestia megaterium NBRC 15308 = ATCC 14581 tRNA 4-thiouridine( 8) synthase ThiI protein. ACCESSION CP035094-4876 PROTEIN_ID QCY27719.1 SOURCE Priestia megaterium NBRC 15308 = ATCC 14581 ORGANISM Priestia megaterium NBRC 15308 = ATCC 14581 Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia. REFERENCE 1 (bases 1 to 5343009) AUTHORS Chen,H. TITLE Bacillus megaterium Genome sequencing and assembly JOURNAL Unpublished REFERENCE 2 (bases 1 to 5343009) AUTHORS Chen,H. TITLE Direct Submission JOURNAL Submitted (14-JAN-2019) Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Zhejiang Tianke High Technology Development Co. Ltd., Huanggushan Road, Hangzhou, Zhejiang 310000, China COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: Unicycler v. Unicycler v0.4.5 Assembly Name :: TK37 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 103.0x Sequencing Technology :: Oxford Nanopore GridION ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 01/14/2019 10:26:59 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 4.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total) :: 5,913 CDSs (total) :: 5,741 Genes (coding) :: 5,596 CDSs (with protein) :: 5,596 Genes (RNA) :: 172 rRNAs :: 15, 13, 13 (5S, 16S, 23S) complete rRNAs :: 15, 13, 13 (5S, 16S, 23S) tRNAs :: 123 ncRNAs :: 8 Pseudo Genes (total) :: 145 CDSs (without protein) :: 145 Pseudo Genes (ambiguous residues) :: 0 of 145 Pseudo Genes (frameshifted) :: 53 of 145 Pseudo Genes (incomplete) :: 80 of 145 Pseudo Genes (internal stop) :: 41 of 145 Pseudo Genes (multiple problems) :: 27 of 145 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Priestia megaterium NBRC 15308 = ATCC 14581" /mol_type="genomic DNA" /strain="ATCC 14581" /culture_collection="ATCC:14581" /type_material="type strain of Bacillus megaterium" /db_xref="taxon:1348623" protein /gene="thiI" /locus_tag="EQG57_25090" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_013059478.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MIYNHILIRY GEISTKGQNR KKFVAQLRKN IVTALSAFQN IHVKYTRDRM YIHLNGENHE 61 PIIERLQQIF GIQSFSLALK VENELEAIQE AALEAMKEFD IEGKTFKVST RRAYKQFPLD 121 TNELNYAVGS HILRNTEHLK VNVKEPSVNV RVEVRSEATY ITCFDYRGAG GLPVGTSGKA 181 MLMLSGGIDS PVAGYLAMKR GLEIEAVHFY SPPFTSERSK QKVIDLTQKL TAYHEKVKLH 241 IVPFTAVQQA IQKQIPENYT MTSTRRMMLR ITDLIREKNE SLAIVTGDSL GQVASQTVES 301 MYAINEVTNT PILRPLVTMD KTEIVEIARK IDTHEISNRP YEDCCTIFTP SSPKTKPKRE 361 KINRYEQFYD FETLIQEAVN NVETLEMTAS SATNTADSVE DLF //