LOCUS KHJ47470.1 1140 aa PRT CON 17-DEC-2014 DEFINITION Trichuris suis hypothetical protein protein. ACCESSION KN538379-226 PROTEIN_ID KHJ47470.1 SOURCE Trichuris suis (pig whipworm) ORGANISM Trichuris suis Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia; Trichinellida; Trichuridae; Trichuris. REFERENCE 1 (bases 1 to 2343098) AUTHORS Mitreva,M. TITLE Draft genome of Trichuris suis JOURNAL Unpublished REFERENCE 2 (bases 1 to 2343098) AUTHORS Mitreva,M., Pepin,K.H., Abubucker,S., Martin,J., Minx,P., Warren,C., Palsikar,V.B., Zhang,X., Rosa,B.A. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (27-JAN-2014) The Genome Institute, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Trichuris suis contaminated a dirt lot located at the USDA, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Disease Laboratory in Beltsville, MD since the early 1960s. Adult worms were isolated for passage from pigs placed on the lot and naturally infected. The T. suis adults were manually removed from the cecum and proximal colon tissue and cultured in vitro to release fertilized eggs that were removed after 24-48 hours and embryonated to an infective stage (Hill et al., Experimental Parasitology 77, 170-178, 1993). The strain has been actively passed in pigs one to two times per year since that time and characterized for pathogenesis in pigs (Mansfield and Urban, Veterinary Immunology and Immunopathology 50, 1-17, 1996). This strain of T. suis has also been used therapeutically in human subjects with inflammatory bowel disease (Trichuris suis therapy in Crohn's disease. Summers RW, Elliott DE, Urban JF Jr, Thompson R, Weinstock JV. Gut. 2005 Jan;54(1):87-90. The Genome Institute collaborators that provided material for the genome/transcriptome sequencing are: USDA - Urban, Jr., J.F., Hill, D. E. and Michigan State University - Mansfield L.S. The repeat library was generated using Repeatmodeler (A. Smit, R. Hubley http://www.systemsbiology.org/). The Ribosomal RNA genes were identified using RNAmmer ((http://www.cbs.dtu.dk/cgi-bin/nph-sw_request?rnammer ) and transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy, 1997). Non-coding RNAs, such as microRNAs, were identified by sequence homology search of the Rfam database (http://selab.janelia.org/software.html). Repeats and predicted RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding genes were predicted using a combination of ab initio programs Snap (I. Korf, 2004), Fgenesh (Softberry, Corp) and Augustus (M. Stanke, et. Al 2008) and the annotation pipeline tool Maker (M. Yandell et. al., 2007) which aligns mRNA, EST and protein information from same species or cross-species to aid in gene structure determination and modifications. A consensus gene set from the above prediction algorithms was generated, using a logical, hierarchical approach developed at the Genome institute. Gene product naming was determined by BER (JCVI: http://ber.sourceforge.net). Our goal is to explore this WGS draft sequence of Trichuris suis to better define proteins involved in nematode parasitism that impact health and disease and are relevant to both host-parasite relationships and basic biological processes. For information regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. For specific questions regarding the Trichuris suis genome project contact Makedonka Mitreva (mmitreva@genome.wustl.edu) at Washington University School of Medicine. The National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) provided funds for this project. ##Genome-Assembly-Data-START## Finishing Goal :: High-Quality Draft Current Finishing Status :: High-Quality Draft Assembly Method :: ALLPATHS_LG v. 2012-11-02 Assembly Name :: T_suis_1.0.allpaths Genome Coverage :: 392x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Trichuris suis" /mol_type="genomic DNA" /submitter_seqid="T_suis-1.0_Cont4" /isolation_source="cecum and proximal colon of infected animals which were naturally infected" /host="Sus scrofa (pig)" /db_xref="taxon:68888" /chromosome="Unknown" /dev_stage="adult" /country="USA: Beltsville, MD" protein /locus_tag="D918_02330" /inference="protein motif:HMMPfam:IPR006935" /inference="protein motif:HMMPfam:IPR018999" /note="KEGG: sce:YMR080C 1.9e-237 NAM7, IFS2, MOF4, SUP113, UPF1; ATP-dependent RNA helicase of the SFI superfamily involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance" /db_xref="InterPro:IPR006935" /db_xref="InterPro:IPR018999" intron_pos 7:1 (1/18) intron_pos 73:0 (2/18) intron_pos 138:2 (3/18) intron_pos 208:2 (4/18) intron_pos 255:0 (5/18) intron_pos 279:2 (6/18) intron_pos 338:1 (7/18) intron_pos 370:1 (8/18) intron_pos 406:2 (9/18) intron_pos 476:0 (10/18) intron_pos 554:2 (11/18) intron_pos 641:0 (12/18) intron_pos 712:1 (13/18) intron_pos 815:0 (14/18) intron_pos 904:0 (15/18) intron_pos 937:1 (16/18) intron_pos 998:1 (17/18) intron_pos 1088:0 (18/18) BEGIN 1 MDCLVTKGKD CITPKMSDYD RYESCAPAST SSYDGDGRIP FIGATQDSDY DYDFTIPSQM 61 DLSQTDARVE GRASPNQAQP LDSAAVRSDR KAVKPHAAVN LPEHACRYCG IYDPKYVLNC 121 NQCKKWFCNG RLKSSAGSHI INHLVRSRHK EVTLHQEGPL GETILECYNC GCRNVFVLGF 181 VAAKSESVIV LLCRQPCAAQ TALKELNWEG GHWKPLIQDR AFLTWLVKMP SVTEQMKARQ 241 VTPLQMNRLE EFWKEKPDAT LEDIENPSID EEPQRVSIKY ESASHYQSIF SPLITLEAEY 301 DRKMKEAQTQ DNVTVRWDIG LNKKHVAFFK LTKVTEGDMK LMQGDELCLK CTFAGYTWSG 361 VGHVIKIPDS HSDTVGLEMK IGTGVPSDVT HGFSVDFVWK STSFDRMRNA LARLVTIETS 421 VSEPIYYRIL GIETEDVVMK IPLPKRFSVR GLPELNHSQV FAVRTVLQRP ISLIQGPPGT 481 GKTVTSATIV HHLAKIYSSP VLVCAPSNIA VDQLAEKIHR TGLKVVRLCA KSREAIESPV 541 SFLSLHYQIR FLHGEEELSK LLQLRDETGE LSSTDEMRFR TLRNKCERDL LRNADVVCCT 601 CVGAGDPRIA QMQFRCVLID ESTQATEPEC MVPVVNGCRQ LILVGDHCQL GPVVMCKKAA 661 RAGLNQSLFE RLVVLGIRPI RLQVQYRMHP ALSAFPSDVF YEGSLQNGVT EAERTLSGFD 721 FTWPEAHSPM FFWLSMGQEE ISGSGTSYLN RTEAANIEKL ATKFLKLGAR PDQIGIITPY 781 EGQRAFIVQY MQFAGTLNKK LYLDIEVASV DAFQGREKDF ILLSCVRSND NLGIGFLNDP 841 RRLNVALTRA RFGLIIVGNP KVLARQQLWQ NLLVFYKEHH LLVEGSLNAL KESAVQFIRT 901 NVQLNSRGSQ DVDSGNEFQS ARSRHMFSNR PMPRLPTSLP ASCRPYRPLN DPLGMIMPER 961 FTTALAASAM NIPLPINMFL PQVPAVNPMM SQQRAAMRSA AIAKAKSRWQ QGASVASPVP 1021 GGMMSDGRQI QQVRDNRPQR GSSNAMSTGH QQAAASSRMS QPFHLSQGMA PSSSNAPMSQ 1081 IASEMSQDQM MDDMLRSQLE TMMLSQDMSY TNSPMVLMQS SASDGVTAAT DKKMFNQTPE //