LOCUS KHJ47246.1 840 aa PRT CON 17-DEC-2014 DEFINITION Trichuris suis ABC transporter transmembrane region protein. ACCESSION KN538379-2 PROTEIN_ID KHJ47246.1 SOURCE Trichuris suis (pig whipworm) ORGANISM Trichuris suis Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia; Trichinellida; Trichuridae; Trichuris. REFERENCE 1 (bases 1 to 2343098) AUTHORS Mitreva,M. TITLE Draft genome of Trichuris suis JOURNAL Unpublished REFERENCE 2 (bases 1 to 2343098) AUTHORS Mitreva,M., Pepin,K.H., Abubucker,S., Martin,J., Minx,P., Warren,C., Palsikar,V.B., Zhang,X., Rosa,B.A. and Wilson,R.K. TITLE Direct Submission JOURNAL Submitted (27-JAN-2014) The Genome Institute, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA COMMENT Trichuris suis contaminated a dirt lot located at the USDA, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Disease Laboratory in Beltsville, MD since the early 1960s. Adult worms were isolated for passage from pigs placed on the lot and naturally infected. The T. suis adults were manually removed from the cecum and proximal colon tissue and cultured in vitro to release fertilized eggs that were removed after 24-48 hours and embryonated to an infective stage (Hill et al., Experimental Parasitology 77, 170-178, 1993). The strain has been actively passed in pigs one to two times per year since that time and characterized for pathogenesis in pigs (Mansfield and Urban, Veterinary Immunology and Immunopathology 50, 1-17, 1996). This strain of T. suis has also been used therapeutically in human subjects with inflammatory bowel disease (Trichuris suis therapy in Crohn's disease. Summers RW, Elliott DE, Urban JF Jr, Thompson R, Weinstock JV. Gut. 2005 Jan;54(1):87-90. The Genome Institute collaborators that provided material for the genome/transcriptome sequencing are: USDA - Urban, Jr., J.F., Hill, D. E. and Michigan State University - Mansfield L.S. The repeat library was generated using Repeatmodeler (A. Smit, R. Hubley http://www.systemsbiology.org/). The Ribosomal RNA genes were identified using RNAmmer ((http://www.cbs.dtu.dk/cgi-bin/nph-sw_request?rnammer ) and transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy, 1997). Non-coding RNAs, such as microRNAs, were identified by sequence homology search of the Rfam database (http://selab.janelia.org/software.html). Repeats and predicted RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding genes were predicted using a combination of ab initio programs Snap (I. Korf, 2004), Fgenesh (Softberry, Corp) and Augustus (M. Stanke, et. Al 2008) and the annotation pipeline tool Maker (M. Yandell et. al., 2007) which aligns mRNA, EST and protein information from same species or cross-species to aid in gene structure determination and modifications. A consensus gene set from the above prediction algorithms was generated, using a logical, hierarchical approach developed at the Genome institute. Gene product naming was determined by BER (JCVI: http://ber.sourceforge.net). Our goal is to explore this WGS draft sequence of Trichuris suis to better define proteins involved in nematode parasitism that impact health and disease and are relevant to both host-parasite relationships and basic biological processes. For information regarding this assembly or project, or any other GSC genome project, please visit our Genome Groups web page (http://genome.wustl.edu/genome_group_index.cgi) and email the designated contact person. For specific questions regarding the Trichuris suis genome project contact Makedonka Mitreva (mmitreva@genome.wustl.edu) at Washington University School of Medicine. The National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) provided funds for this project. ##Genome-Assembly-Data-START## Finishing Goal :: High-Quality Draft Current Finishing Status :: High-Quality Draft Assembly Method :: ALLPATHS_LG v. 2012-11-02 Assembly Name :: T_suis_1.0.allpaths Genome Coverage :: 392x Sequencing Technology :: Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Trichuris suis" /mol_type="genomic DNA" /submitter_seqid="T_suis-1.0_Cont4" /isolation_source="cecum and proximal colon of infected animals which were naturally infected" /host="Sus scrofa (pig)" /db_xref="taxon:68888" /chromosome="Unknown" /dev_stage="adult" /country="USA: Beltsville, MD" protein /locus_tag="D918_02106" /inference="protein motif:HMMPfam:IPR001140" /inference="protein motif:HMMPfam:IPR003439" /note="KEGG: phu:Phum_PHUM351610 1.2e-182 multidrug resistance protein, putative" /db_xref="InterPro:IPR001140" /db_xref="InterPro:IPR003439" intron_pos 12:0 (1/10) intron_pos 138:0 (2/10) intron_pos 194:0 (3/10) intron_pos 253:1 (4/10) intron_pos 267:2 (5/10) intron_pos 567:2 (6/10) intron_pos 614:0 (7/10) intron_pos 648:0 (8/10) intron_pos 749:0 (9/10) intron_pos 804:2 (10/10) BEGIN 1 MRKLEGSVAV QGTISYVPQQ AWILNATVRD NILFTKPNRP NFYTQVVKAC SLDVDIEEMS 61 EKSDTEIGEK VCCNQLLLFV QLFFDIYFRV SYKYSNKILI QKISLVEKIF FLSKECPAVF 121 AVSILAAFPA LTSSFFQGIN LSGGQKQRLS IARAVYQYTD IYLFDDPLSA VDSRVGRHIF 181 DNVIGRKGLL KDKTRVFVTH ALAFLKDVDL IVIMDQGRIK KVGTPSQLLT EWDGLSEFLE 241 EKNDNAEEPK LSDEESGDEN AEMLRSRRNT VTSVSSSKSS KLDARNSLRE SNAIANGPAK 301 SHENEWNIKR KRLIEEETAE TGRVKAHVYK LYVQSIGFLS SFVVFILYMV SGGLSVGSSI 361 WLAEWSEDSV CSAKNRSDCV QTDTRIGVYA ALGIGQALFV FAAALLMMLC MVASSANLHE 421 RLLHNLLRVP MSFYETTPLG RVLNRIGKDI DVVDNTLPAA VRTWMNCLVQ IIATLVIIAM 481 NMKAFAAAII PLAIFYYAVQ RFYVSSSRQL KRMESVSRSP IYSLFQEVIQ GAVSIRAYRA 541 QKHFRDMFDK HVDANQMTYY PMIVSNRFRW LAVRLEFIGN LLVFLAAIFA VYNREEGILS 601 AGMVGLAVTY ALSITQTLNW VVRMTSDLET NIVSVERIVE YMNVPTEADW TTEYRPPHNW 661 PSMGRVEFDR YAMRYRPGLD LVLKGISFVA HGGEKVGIVG RTGAGKSSIT LALFRIVEPV 721 EGSIVIDSVD IGLIGLHDLR SRISIIPQEP VLFCGSIRMN IDPTGQKNDD EIWTALEHAH 781 LKSFISTLPD KLDHEVVEGG ENLRLLVLSN GQVVDNDSPA NLLTRLDGEF YAMAKEANIV //