LOCUS DAA09066.1 1104 aa PRT PLN 23-JAN-2024
DEFINITION Saccharomyces cerevisiae S288C GTPase-activating protein
BUD2 protein.
ACCESSION BK006944-124
PROTEIN_ID DAA09066.1
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 666816)
AUTHORS Dujon,B., Alexandraki,D., Andre,B., Ansorge,W., Baladron,V.,
Ballesta,J.P., Banrevi,A., Bolle,P.A., Bolotin-Fukuhara,M.,
Bossier,P. et al.
TITLE Complete DNA sequence of yeast chromosome XI
JOURNAL Nature 369 (6479), 371-378 (1994)
PUBMED 8196765
REFERENCE 2 (bases 1 to 666816)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 3 (bases 1 to 666816)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 4 (bases 1 to 666816)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REFERENCE 5 (bases 1 to 666816)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 6 (bases 1 to 666816)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
COMMENT THIRD PARTY DATABASE: This TPA record uses data from
DDBJ/EMBL/GenBank entries JF740677.1, Z28001.1, Z28002.1, Z28003.1,
Z28004.1, Z28005.1, Z28006.1, Z28007.1, Z28008.1, Z28009.1,
Z28010.1, Z28011.1, Z28012.1, Z28013.1, Z28014.1, Z28015.1,
Z28016.1, Z28017.1, Z28018.1, Z28019.1, Z28020.1, Z28021.1,
Z28022.1, Z28023.1, Z28024.1, Z28025.1, Z28026.1, Z28027.1,
Z28028.1, Z28029.1, Z28031.1, Z28032.1, Z28033.1, Z28034.1,
Z28035.1, Z28038.1, Z28039.1, Z28040.1, Z28041.1, Z28042.1,
Z28043.1, Z28044.1, Z28045.1, Z28046.1, Z28047.1, Z28048.1,
Z28049.1, Z28050.1, Z28051.1, Z28052.1, Z28054.1, Z28055.1,
Z28056.1, Z28057.1, Z28058.1, Z28059.1, Z28060.1, Z28061.1,
Z28062.1, Z28063.1, Z28064.1, Z28065.1, Z28066.1, Z28067.1,
Z28068.1, Z28069.1, Z28070.1, Z28071.1, Z28072.1, Z28073.1,
Z28074.1, Z28075.1, Z28077.1, Z28078.1, Z28079.1, Z28080.1,
Z28081.1, Z28082.1, Z28084.1, Z28085.1, Z28086.1, Z28087.1,
Z28088.1, Z28089.1, Z28090.1, Z28091.1, Z28092.1, Z28093.1,
Z28094.1, Z28095.1, Z28096.1, Z28097.1, Z28098.1, Z28099.1,
Z28100.1, Z28101.1, Z28102.1, Z28103.1, Z28104.1, Z28105.1,
Z28106.1, Z28107.1, Z28108.1, Z28109.1, Z28110.1, Z28111.1,
Z28113.1, Z28114.1, Z28115.1, Z28117.1, Z28118.1, Z28120.1,
Z28121.1, Z28122.1, Z28123.1, Z28125.1, Z28126.1, Z28127.1,
Z28128.1, Z28129.1, Z28130.1, Z28131.1, Z28133.1, Z28134.1,
Z28135.1, Z28137.1, Z28138.1, Z28139.1, Z28140.1, Z28141.1,
Z28142.1, Z28143.1, Z28144.1, Z28145.1, Z28146.1, Z28148.1,
Z28149.1, Z28150.1, Z28151.1, Z28152.1, Z28154.1, Z28155.1,
Z28156.1, Z28157.1, Z28158.1, Z28159.1, Z28160.1, Z28161.1,
Z28162.1, Z28163.1, Z28164.1, Z28165.1, Z28166.1, Z28167.1,
Z28168.1, Z28169.1, Z28171.1, Z28172.1, Z28173.1, Z28174.1,
Z28175.1, Z28176.1, Z28177.1, Z28179.1, Z28180.1, Z28181.1,
Z28182.1, Z28183.1, Z28184.1, Z28185.1, Z28186.1, Z28187.1,
Z28188.1, Z28189.1, Z28190.1, Z28191.1, Z28192.1, Z28193.1,
Z28194.1, Z28195.1, Z28196.1, Z28197.1, Z28198.1, Z28200.1,
Z28201.1, Z28202.1, Z28203.1, Z28204.1, Z28205.1, Z28206.1,
Z28207.1, Z28208.1, Z28209.1, Z28210.1, Z28211.1, Z28212.1,
Z28213.1, Z28214.1, Z28215.1, Z28216.1, Z28217.1, Z28218.1,
Z28219.1, Z28220.1, Z28221.1, Z28222.1, Z28223.1, Z28224.1,
Z28225.1, Z28226.1, Z28227.1, Z28228.1, Z28229.1, Z28230.1,
Z28231.1, Z28232.1, Z28233.1, Z28234.1, Z28235.1, Z28236.1,
Z28237.1, Z28238.1, Z28239.1, Z28240.1, Z28241.1, Z28242.1,
Z28243.1, Z28244.1, Z28245.1, Z28246.1, Z28247.1, Z28248.1,
Z28249.1, Z28250.1, Z28251.1, Z28252.1, Z28253.1, Z28254.1,
Z28255.1, Z28256.1, Z28257.1, Z28258.1, Z28259.1, Z28260.1,
Z28261.1, Z28262.1, Z28263.1, Z28264.1, Z28265.1, Z28266.1,
Z28267.1, Z28268.1, Z28269.1, Z28270.1, Z28271.1, Z28272.1,
Z28273.1, Z28274.1, Z28275.1, Z28276.1, Z28277.1, Z28278.1,
Z28279.1, Z28280.1, Z28281.1, Z28282.1, Z28283.1, Z28284.1,
Z28285.1, Z28286.1, Z28287.1, Z28288.1, Z28289.1, Z28290.1,
Z28291.1, Z28292.1, Z28293.1, Z28294.1, Z28295.1, Z28296.1,
Z28297.1, Z28298.1, Z28299.1, Z28300.1, Z28301.1, Z28302.1,
Z28303.1, Z28304.1, Z28305.1, Z28306.1, Z28307.1, Z28308.1,
Z28309.1, Z28310.1, Z28311.1, Z28312.1, Z28313.1, Z28314.1,
Z28315.1, Z28316.1, Z28317.1, Z28318.1, Z28319.1, Z28320.1,
Z28321.1, Z28322.1, Z28323.1, Z28324.1, Z28325.1, Z28326.1,
Z28327.1, Z28328.1, Z28329.1 and Z28330.1
On Apr 18, 2011 this sequence version replaced BK006944.1.
Chromosome XI sequence was verified by resequencing. The sequence
was mapped to the previous GenBank records except for a 500 bp
region. That region was determined by sequencing a descendant of
the S288C strain, AB972, and is present in GenBank as JF740677.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="XI"
protein /gene="BUD2"
/locus_tag="YKL092C"
/gene_synonym="CLA2"
/gene_synonym="ERC25"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10444589]"
/experiment="EXISTENCE:direct assay:GO:0005096 GTPase
activator activity [PMID:8371782]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:10444589|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007120 axial
cellular bud site selection [PMID:2065354|PMID:22558355]"
/experiment="EXISTENCE:mutant phenotype:GO:0007121 bipolar
cellular bud site selection [PMID:2065354]"
/experiment="EXISTENCE:mutant phenotype:GO:0007163
establishment or maintenance of cell polarity
[PMID:23783029]"
/experiment="EXISTENCE:mutant phenotype:GO:0030447
filamentous growth [PMID:15645503]"
/experiment="EXISTENCE:mutant phenotype:GO:0031578 mitotic
spindle orientation checkpoint signaling [PMID:22558355]"
/experiment="EXISTENCE:mutant phenotype:GO:0036267
invasive filamentous growth [PMID:23783029]"
/note="GTPase activating factor for Rsr1p/Bud1p; plays a
role in spindle position checkpoint distinct from its role
in bud site selection; required for both axial and bipolar
budding patterns; mutants exhibit random budding in all
cell types; contains two PH-like domains"
/db_xref="SGD:S000001575"
BEGIN
1 MSSNNEPAQS RTSYFKLNEF LSNVKHYKNT FKGEIQWCNN LSLNDWKTHY LQITSTGALT
61 HSIDELTADS TNIQPIIKHL QQCRIEIIKD KHSSFKDINA NCNFIIQVNT SGKDNKVYLR
121 VKSWSDFKKL LTCLIWWSSM KTNGIFNKFQ VSRPLEFKSK KMAKPESLLV YKLNVFGPIV
181 KNIVLPPATN ILESPDIINN DDNSVGWFSA MGVLKSNGML DLLLQSDGSL IYSLNISQLL
241 RSEIRILDSS VLQSENSLFL GELPLLRSQL GLEKFRIENI ASAATNSSDI SQEIIVEFPL
301 RIDLEDCFIA LQSFARSEYL SITGSDKSND MKISNSFKIS ILEANFQSIN LNDKNNTPWS
361 IFTDITAWGH TWARTSMVSN SSNPFWREEF QFNELLRLTN SYLEIKQLFH DLNNKKRLRL
421 IGKIKITQEI INDTRYNKET RLPIMDVDNK NFQIGTICIK ISSNLNFILP STNFVKLEKL
481 LMNANLSMVS NLIYKSSSSM ENDNKLTQTS IIFLDIFQSL SRIEEWFHVL IDKELAKIDG
541 TVSRINQKNL DSKHVFNSLF RGNSILTKSI EQYFFRVGNE YLSKALSAIL KEIIESNKSC
601 ELDPARVKEK DEVKKRKIIA DNYKRLYSWV TKIWKRLYAT SNDLPIEIRN VLKIFRQKLE
661 IICIDDTLQI ILNGISGLLF LRFFCPVILN PKLFKYVSQN LNETARRNLT LISKVLLNLS
721 TLTQFANKEP WLMKMNNFID KRHNDLLDYI DKMTQKKLDF NSKILNLSST ISRPKLAIEQ
781 TMLDDLPQIP YLLDKNLRET EFVNLIVNFS QEDMTKMEKY NHMDNGGKGE LIEEEGLLSG
841 SSLNLSVDKK DLDSPIEVKP EIGELEFEKI TENNTEIFGD DLMNLLKSDD VGSRSRDLDN
901 GANSGIKFNS IIPKAEEEKH AMKELEQESC LLYNRINHIR KRLSGYECAS STLFEDKKYS
961 ISLSHKIFYE EIKEGKEIVL KLLNKPTNEN SSARLQKFFT KGVSSKSNNT VGDSYCKFLT
1021 IDVSDENPKS SNKTSVHGTS SENGAKDDYL TLPNSQGKGN LGNRFSPTKL SRIMRKPPNA
1081 DVPKEQNSRK LTRWFKKKKE TGGS
//