LOCUS DAA08990.1 828 aa PRT PLN 23-JAN-2024 DEFINITION Saccharomyces cerevisiae S288C Lst4p protein. ACCESSION BK006944-45 PROTEIN_ID DAA08990.1 SOURCE Saccharomyces cerevisiae S288C ORGANISM Saccharomyces cerevisiae S288C Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. REFERENCE 1 (bases 1 to 666816) AUTHORS Dujon,B., Alexandraki,D., Andre,B., Ansorge,W., Baladron,V., Ballesta,J.P., Banrevi,A., Bolle,P.A., Bolotin-Fukuhara,M., Bossier,P. et al. TITLE Complete DNA sequence of yeast chromosome XI JOURNAL Nature 369 (6479), 371-378 (1994) PUBMED 8196765 REFERENCE 2 (bases 1 to 666816) AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B., Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M., Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and Oliver,S.G. TITLE Life with 6000 genes JOURNAL Science 274 (5287), 546 (1996) PUBMED 8849441 REFERENCE 3 (bases 1 to 666816) AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M., Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S., Weng,S. and Cherry,J.M. TITLE New data and collaborations at the Saccharomyces Genome Database: updated reference genome, alleles, and the Alliance of Genome Resources JOURNAL Genetics 220 (4) (2022) PUBMED 34897464 REFERENCE 4 (bases 1 to 666816) CONSRTM Saccharomyces Genome Database TITLE Direct Submission JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA REFERENCE 5 (bases 1 to 666816) CONSRTM Saccharomyces Genome Database TITLE Direct Submission JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA REMARK Sequence update by submitter REFERENCE 6 (bases 1 to 666816) CONSRTM Saccharomyces Genome Database TITLE Direct Submission JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford University, Stanford, CA 94305-5120, USA REMARK Protein update by submitter COMMENT THIRD PARTY DATABASE: This TPA record uses data from DDBJ/EMBL/GenBank entries JF740677.1, Z28001.1, Z28002.1, Z28003.1, Z28004.1, Z28005.1, Z28006.1, Z28007.1, Z28008.1, Z28009.1, Z28010.1, Z28011.1, Z28012.1, Z28013.1, Z28014.1, Z28015.1, Z28016.1, Z28017.1, Z28018.1, Z28019.1, Z28020.1, Z28021.1, Z28022.1, Z28023.1, Z28024.1, Z28025.1, Z28026.1, Z28027.1, Z28028.1, Z28029.1, Z28031.1, Z28032.1, Z28033.1, Z28034.1, Z28035.1, Z28038.1, Z28039.1, Z28040.1, Z28041.1, Z28042.1, Z28043.1, Z28044.1, Z28045.1, Z28046.1, Z28047.1, Z28048.1, Z28049.1, Z28050.1, Z28051.1, Z28052.1, Z28054.1, Z28055.1, Z28056.1, Z28057.1, Z28058.1, Z28059.1, Z28060.1, Z28061.1, Z28062.1, Z28063.1, Z28064.1, Z28065.1, Z28066.1, Z28067.1, Z28068.1, Z28069.1, Z28070.1, Z28071.1, Z28072.1, Z28073.1, Z28074.1, Z28075.1, Z28077.1, Z28078.1, Z28079.1, Z28080.1, Z28081.1, Z28082.1, Z28084.1, Z28085.1, Z28086.1, Z28087.1, Z28088.1, Z28089.1, Z28090.1, Z28091.1, Z28092.1, Z28093.1, Z28094.1, Z28095.1, Z28096.1, Z28097.1, Z28098.1, Z28099.1, Z28100.1, Z28101.1, Z28102.1, Z28103.1, Z28104.1, Z28105.1, Z28106.1, Z28107.1, Z28108.1, Z28109.1, Z28110.1, Z28111.1, Z28113.1, Z28114.1, Z28115.1, Z28117.1, Z28118.1, Z28120.1, Z28121.1, Z28122.1, Z28123.1, Z28125.1, Z28126.1, Z28127.1, Z28128.1, Z28129.1, Z28130.1, Z28131.1, Z28133.1, Z28134.1, Z28135.1, Z28137.1, Z28138.1, Z28139.1, Z28140.1, Z28141.1, Z28142.1, Z28143.1, Z28144.1, Z28145.1, Z28146.1, Z28148.1, Z28149.1, Z28150.1, Z28151.1, Z28152.1, Z28154.1, Z28155.1, Z28156.1, Z28157.1, Z28158.1, Z28159.1, Z28160.1, Z28161.1, Z28162.1, Z28163.1, Z28164.1, Z28165.1, Z28166.1, Z28167.1, Z28168.1, Z28169.1, Z28171.1, Z28172.1, Z28173.1, Z28174.1, Z28175.1, Z28176.1, Z28177.1, Z28179.1, Z28180.1, Z28181.1, Z28182.1, Z28183.1, Z28184.1, Z28185.1, Z28186.1, Z28187.1, Z28188.1, Z28189.1, Z28190.1, Z28191.1, Z28192.1, Z28193.1, Z28194.1, Z28195.1, Z28196.1, Z28197.1, Z28198.1, Z28200.1, Z28201.1, Z28202.1, Z28203.1, Z28204.1, Z28205.1, Z28206.1, Z28207.1, Z28208.1, Z28209.1, Z28210.1, Z28211.1, Z28212.1, Z28213.1, Z28214.1, Z28215.1, Z28216.1, Z28217.1, Z28218.1, Z28219.1, Z28220.1, Z28221.1, Z28222.1, Z28223.1, Z28224.1, Z28225.1, Z28226.1, Z28227.1, Z28228.1, Z28229.1, Z28230.1, Z28231.1, Z28232.1, Z28233.1, Z28234.1, Z28235.1, Z28236.1, Z28237.1, Z28238.1, Z28239.1, Z28240.1, Z28241.1, Z28242.1, Z28243.1, Z28244.1, Z28245.1, Z28246.1, Z28247.1, Z28248.1, Z28249.1, Z28250.1, Z28251.1, Z28252.1, Z28253.1, Z28254.1, Z28255.1, Z28256.1, Z28257.1, Z28258.1, Z28259.1, Z28260.1, Z28261.1, Z28262.1, Z28263.1, Z28264.1, Z28265.1, Z28266.1, Z28267.1, Z28268.1, Z28269.1, Z28270.1, Z28271.1, Z28272.1, Z28273.1, Z28274.1, Z28275.1, Z28276.1, Z28277.1, Z28278.1, Z28279.1, Z28280.1, Z28281.1, Z28282.1, Z28283.1, Z28284.1, Z28285.1, Z28286.1, Z28287.1, Z28288.1, Z28289.1, Z28290.1, Z28291.1, Z28292.1, Z28293.1, Z28294.1, Z28295.1, Z28296.1, Z28297.1, Z28298.1, Z28299.1, Z28300.1, Z28301.1, Z28302.1, Z28303.1, Z28304.1, Z28305.1, Z28306.1, Z28307.1, Z28308.1, Z28309.1, Z28310.1, Z28311.1, Z28312.1, Z28313.1, Z28314.1, Z28315.1, Z28316.1, Z28317.1, Z28318.1, Z28319.1, Z28320.1, Z28321.1, Z28322.1, Z28323.1, Z28324.1, Z28325.1, Z28326.1, Z28327.1, Z28328.1, Z28329.1 and Z28330.1 On Apr 18, 2011 this sequence version replaced BK006944.1. Chromosome XI sequence was verified by resequencing. The sequence was mapped to the previous GenBank records except for a 500 bp region. That region was determined by sequencing a descendant of the S288C strain, AB972, and is present in GenBank as JF740677. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-4-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Saccharomyces cerevisiae S288C" /mol_type="genomic DNA" /strain="S288C" /db_xref="taxon:559292" /chromosome="XI" protein /gene="LST4" /locus_tag="YKL176C" /experiment="EXISTENCE:direct assay:GO:0005096 GTPase activator activity [PMID:26387955]" /experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm [PMID:26387955]" /experiment="EXISTENCE:direct assay:GO:0005774 vacuolar membrane [PMID:26387955]" /experiment="EXISTENCE:direct assay:GO:0005829 cytosol [PMID:26928762]" /experiment="EXISTENCE:direct assay:GO:1990877 FNIP-folliculin RagC/D GAP [PMID:26387955]" /experiment="EXISTENCE:mutant phenotype:GO:1904263 positive regulation of TORC1 signaling [PMID:26387955]" /note="Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface" /db_xref="SGD:S000001659" BEGIN 1 MLGNLLRNKT SSSGFEKSSE HSDFSSVVPN VPVYCKAAST GTTKTAAGAL LDTAVNVEKP 61 SEMLSTTSPP ILDHISDDLK LKLFGSRDIP YSRPIDTLQN NGGLGTDKIT SINEKTYAFR 121 ILIIEEAGQM ACRNNYRDIF DYTTSKISNS MEQIRPSELK EYIFGSPVRS SDLTQCDKIR 181 TIPNSDLVLI TRIFYYTHQY NRIAISLCIP RILLPVVAES WSSISSWLTQ TQKMLIGFLT 241 KNRIMQENTG NYSNNSVIKL SNIDIRTHYP KEIEIMVQTL QKRVIPGLRS MSEIPRLFLY 301 PETFKEFVHV WFKSIFNWIE IKDGPKLGFL PLLMAMIISD YRHTIRELKT SKIVILSGNM 361 VVANKLLFIL SALLEPKYKG QITIRRENIR SDSSAVSRNK SNNNFVDKPE TELSTLTSTD 421 NLLSRTENNS NHNYNNSNVS SNSIGSPNFH SLRKGWQIPN RRNSNTSVSV SSSESLAEVI 481 QPSSFKSGSS SLHYLSSSIS SQPGSYGSWF NKRPTISQFF QPSPSLKHNE SWERLQTTAG 541 NMQRTSSSSS LQQATSRLSL TTPQQSPSIS EYDEYPWMGT PGSPNVGDVS HAPPLVKNIS 601 YKFPLKNVEL KRDCQRISQD DLLDEAFERI CQPSLADLNS TYEIFPGNSS YADILTTDSD 661 IDDGLMNKPL ELLPKYTMYL THFNNFFQLQ ACPAGQESES RITNSMKIDL LKADYTRSLL 721 VSLRSRDIRD VALKREFTGN NNNNSNQNIY DENFVGKRKY VLKQKTRKIF SCGKIGKLST 781 SLENCVNFVE NSIKSAMMLY DDNGIDSELR DSEALRIFSS LVHYCNAG //