LOCUS       CDQ48116.1               918 aa    PRT              BCT 04-FEB-2016
DEFINITION  Vibrio anguillarum EmpA processing protease; protein.
ACCESSION   LK021129-219
PROTEIN_ID  CDQ48116.1
SOURCE      Vibrio anguillarum
  ORGANISM  Vibrio anguillarum
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 1187342)
  AUTHORS   Holm K.
  JOURNAL   Submitted (26-MAR-2014) to the INSDC. Norstruct, Dept of Chemistry,
            University of Tromso, Science Park 3, NO-9037 Tromso, NORWAY.
REFERENCE   2
  AUTHORS   Holm K.O., Nilsson K., Hjerde E., Willassen N.P., Milton D.L.
  TITLE     Complete genome sequence of Vibrio anguillarum strain NB10, a
            virulent isolate from the Gulf of Bothnia
  JOURNAL   Stand Genomic Sci 10, 60-60(2015).
   PUBMED   26380645
FEATURES             Qualifiers
     source          /organism="Vibrio anguillarum"
                     /chromosome="2"
                     /host="Rainbow trout"
                     /strain="NB10"
                     /mol_type="genomic DNA"
                     /country="Sweden:Baltic Sea, Norrbyn Umeaa"
                     /isolation_source="clinical isolate, Rainbow trout"
                     /serovar="O1"
                     /db_xref="taxon:55601"
     protein         /transl_table=11
                     /gene="empA-p"
                     /gene_synonym="epp"
                     /locus_tag="VANGNB10_cII0219c"
                     /old_locus_tag="TVA2585"
                     /function="Ref: The zinc metalloprotease EmpA is a
                     virulence factor for the fish pathogen Vibrio anguillarum.
                     Previous studies demonstrated that EmpA is secreted as a
                     46-kDa proenzyme that is activated extracellularly by the
                     removal of an 10-kDa propeptide. We hypothesized that a
                     specific protease is responsible for processing secreted
                     pro-EmpA into mature EmpA. To identify the protease
                     responsible for processing pro-EmpA, a minitransposon
                     mutagenesis (using mini-Tn10Km) clone bank of V.
                     anguillarum was screened for reduced protease activity due
                     to insertions in undescribed genes. One mutant with
                     reduced protease activity was identified. The region
                     containing the mini-Tn10Km was cloned, sequenced, and
                     found to contain epp, an open reading frame encoding a
                     putative protease. Further characterization of epp was
                     done using strain M101,created by single-crossover
                     insertional mutagenesis. Protease activity was absent in
                     M101 cultures even when empA protease activity was induced
                     by salmon gastrointestinal mucus. When the epp mutation
                     was complemented with a wild-type copy of epp (M102),
                     protease activity was restored. Western blot analysis of
                     sterile filtered culture supernatants from wild-type
                     (M93Sm) cells,M101 cells, and M102 cells revealed that
                     only pro-EmpA was present in M101supernatants; both
                     pro-EmpA and mature EmpA were detected in M93Sm and M102
                     supernatants. When sterile filtered culture supernatants
                     from the empA mutant strain (M99) and M101 were mixed,
                     protease activity was restored. Western blot analysis
                     revealed that pro-EmpA in M101 culture supernatant was
                     processed to mature EmpA only after mixing with M99
                     culture supernatant. These data show that Epp is the
                     EmpA-processing protease."
                     /note="user locus_tag: VANGcII0219c"
                     /note="Similar to Vibrio anguillarum (Listonella
                     anguillarum) epp subname: full=empa processing protease
                     UniProt:B5LBF9 (EMBL:EU650390) (918 aa) fasta scores:
                     E()=0, 99.3% id in 918 aa, and to Vibrio anguillarum
                     (Listonella anguillarum) epp subname: full=empa processing
                     protease UniProt:B5LBF9 (EMBL:EU650390) (918 aa) fasta
                     scores: E()=0, 99.3% id in 918 aa"
BEGIN
        1 MKHIRKTLLA SAMVTLFNVS AYAHTPIDLG VVNEDKLIEM LVRNGVVDAN ATDAIKRDAL
       61 DRYLEQKIKS GFHGDAQFGK QALEQRARVL KTIEKQKGHQ KASVFALDIS TKRTDKVLAL
      121 LIDFPDLPWN DNRLTKEHTE MLYASYNPPH YQDLLFSDAG YAGPNGENFI SMRQYYESES
      181 GQSYSVAGQA AGWYRASKNA AFYGGNSATT DKDMNAQELV REALNQLAQD PSINLADYDI
      241 EDRYDYNGNG NFREPDGVID HLMVFHASVG EEAGGGVLGP DAIWSHRYNL GQPYVLQGTK
      301 STLPDRFNGQ YAAFDYTMQP IDAAAGVCAH EYGHDLGLPD EYDTQYTGKG EPVSYWSIMS
      361 SGSWAGKIGG TQPTAFSSWS KQFLQKAVGG RWINDDQISI AELEKNPRVF TLFQTTDNAR
      421 PNMVKIDLPL KRVDGVKPYQ GSSAFYSNRG DDLKNSMSRK LSIPTGEQAT LNFKAWYQIE
      481 KDYDFARVLI NGKAIAGNIT TMDDPYNTGL VPAIAGQSDG WVDAIFDVSQ WAGQEVTLSF
      541 DYVTDGGLAM EGLYLDNLQL EVDGQQTLID DAEGTSTFAF KGYTTNNGFH EAPHYYLLQW
      601 RSHNDVDQGL ANIKRMGQLM TFEPGLLVWY VDESYTDNWV GNHPGEGWLG VVDADQNALV
      661 WSKTGEAAQT RFQVRDATFS LQDQTPLRLV AADGDVLEDT SLIGNGNFSD GADYSSPLVP
      721 DAGRKLTEFG LMIDILNQGQ DNEYGVIRLS KVSEQNQAPV ANFELKTDGL SVSAFNYSYD
      781 EDGELVSYAW DFGNGQMSSE MAPSWSYTKA GQYTVSLTVT DDKGATNTTT RTTQVEVPNA
      841 LPQASAKYIH LGRWVTMWST SSDSDGRIVD TEWTLPNGKI KRGKLFTAIF PSSGKHEVKI
      901 KVMDDKGATV TNTIVVNL
//