LOCUS CCP46529.1 432 aa PRT BCT 27-FEB-2015
DEFINITION Mycobacterium tuberculosis H37Rv Glutamate--cysteine ligase
GshA (gamma-glutamylcysteine synthetase) (gamma-ECS) (
GCS) (gamma-glutamyl-L-cysteine synthetase) protein.
ACCESSION AL123456-3807
PROTEIN_ID CCP46529.1
SOURCE Mycobacterium tuberculosis H37Rv
ORGANISM Mycobacterium tuberculosis H37Rv
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.,
Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III.,
Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
Connor R., Davies R., Devlin K., Feltwell T., Gentles S.,
Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J.,
Moule S., Murphy L., Oliver K., Osborne J., Quail M.A.,
Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J.,
Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S.,
Barrell B.G.
TITLE Deciphering the biology of Mycobacterium tuberculosis from the
complete genome sequence
JOURNAL Nature 393(6685), 537-544(1998).
PUBMED 9634230
REMARK Erratum:[Nature 1998 Nov 12;396(6707):190]
REFERENCE 2
AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T.
TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis
H37Rv
JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002).
PUBMED 12368430
REFERENCE 3
AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T.
TITLE TubercuList--10 years after
JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011).
PUBMED 20980199
REFERENCE 4 (bases 1 to 4411529)
AUTHORS Parkhill J.
JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the
Mycobacterium tuberculosis sequencing and mapping teams, Sanger
Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28
rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail:
parkhill@sanger.ac.uk
REFERENCE 5 (bases 1 to 4411532)
AUTHORS Lew J.M.
JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique
Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss
Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva
4, SWITZERLAND
COMMENT On or before Feb 1, 2013 this sequence version replaced
gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250,
gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756,
gi:38490319, gi:41352785, gi:38490370, gi:41353971.
Note:
This annotation is from the TubercuList website, Release 26, Dec
2012 (URL: http://tuberculist.epfl.ch) (email:
tuberculist@epfl.ch).
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis H37Rv"
/strain="H37Rv"
/mol_type="genomic DNA"
/db_xref="taxon:83332"
protein /transl_table=11
/gene="gshA"
/locus_tag="Rv3704c"
/note="Rv3704c, (MTV025.052c), len: 432 aa. Possible
gshA,glutamate--cysteine ligase, similar to many e.g.
Q9A2Z2|CC3414 glutamate--cysteine ligase from Caulobacter
crescentus (453 aa), FASTA scores: opt: 404, E():
5.9e-17,(30.45% identity in 312 aa overlap); Q9SEH0|GSH1
gamma-glutamylcysteinyl synthetase precursor from Pisum
sativum (Garden pea) (499 aa), FASTA scores: opt: 400,
E(): 1.1e-16, (26.4% identity in 439 aa overlap);
Q9RH09|GSH gamma-glutamylcysteine synthetase from
Zymomonas mobilis (462 aa), FASTA scores: opt: 397, E():
1.6e-16, (28.95% identity in 304 aa overlap);
P46309|GSH1_ARATH|GSH1|AT4G23100|F7H19.290
glutamate--cysteine ligase from Arabidopsis thaliana
(Mouse-ear cress) (522 aa), FASTA scores: opt: 395, E():
2.3e-16, (27.25% identity in 385 aa overlap); etc. But
note that this putative protein is also similar to
Q9JMV4|GSHA putative glutathione synthetase (fragment)
from Bradyrhizobium japonicum (460 aa), FASTA scores: opt:
498,E(): 1.3e-22, (33.35% identity in 333 aa overlap) (no
significant publications found (August 2001)). Nucleotide
position 4147070 in the genome sequence has been
corrected,A:G resulting in L373L."
/db_xref="EnsemblGenomes-Gn:Rv3704c"
/db_xref="EnsemblGenomes-Tr:CCP46529"
/db_xref="GOA:P9WPK7"
/db_xref="InterPro:IPR006336"
/db_xref="InterPro:IPR014746"
/db_xref="InterPro:IPR017809"
/db_xref="InterPro:IPR035434"
/db_xref="UniProtKB/Swiss-Prot:P9WPK7"
/experiment="EXISTENCE: identified in proteomics study"
BEGIN
1 MTLAAMTAAA SQLDNAAPDD VEITDSSAAA EYIADGCLVD GPLGRVGLEM EAHCFDPADP
61 FRRPSWEEIT EVLEWLSPLP GGSVVSVEPG GAVELSGPPA DGVLAAIGAM TRDQAVLRSA
121 LANAGLGLVF LGADPLRSPV RVNPGARYRA MEQFFAASHS GVPGAAMMTS TAAIQVNLDA
181 GPQEGWAERV RLAHALGPTM IAIAANSPML GGRFSGWQST RQRVWGQMDS ARCGPILGAS
241 GDHPGIDWAK YALKAPVMMV RSPDTQDTRA VTDYVPFTDW VDGRVLLDGR RATVADLVYH
301 LTTLFPPVRP RQWLEIRYLD SVPDEVWPAV VFTLVTLLDD PVAADLAVDA VEPVATAWDT
361 AARIGLADRR LYLAANRCLA IAARRVPTEL IGAMQRLVDH VDRGVCPADD FSDRVIAGGI
421 ASAVTGMMHG AS
//