LOCUS CCP46271.1 455 aa PRT BCT 27-FEB-2015
DEFINITION Mycobacterium tuberculosis H37Rv Probable membrane-anchored
mycosin MycP4 (serine protease) (subtilisin-like protease)
(subtilase-like) (mycosin-4) protein.
ACCESSION AL123456-3549
PROTEIN_ID CCP46271.1
SOURCE Mycobacterium tuberculosis H37Rv
ORGANISM Mycobacterium tuberculosis H37Rv
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.,
Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III.,
Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
Connor R., Davies R., Devlin K., Feltwell T., Gentles S.,
Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J.,
Moule S., Murphy L., Oliver K., Osborne J., Quail M.A.,
Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J.,
Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S.,
Barrell B.G.
TITLE Deciphering the biology of Mycobacterium tuberculosis from the
complete genome sequence
JOURNAL Nature 393(6685), 537-544(1998).
PUBMED 9634230
REMARK Erratum:[Nature 1998 Nov 12;396(6707):190]
REFERENCE 2
AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T.
TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis
H37Rv
JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002).
PUBMED 12368430
REFERENCE 3
AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T.
TITLE TubercuList--10 years after
JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011).
PUBMED 20980199
REFERENCE 4 (bases 1 to 4411529)
AUTHORS Parkhill J.
JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the
Mycobacterium tuberculosis sequencing and mapping teams, Sanger
Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28
rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail:
parkhill@sanger.ac.uk
REFERENCE 5 (bases 1 to 4411532)
AUTHORS Lew J.M.
JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique
Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss
Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva
4, SWITZERLAND
COMMENT On or before Feb 1, 2013 this sequence version replaced
gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250,
gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756,
gi:38490319, gi:41352785, gi:38490370, gi:41353971.
Note:
This annotation is from the TubercuList website, Release 26, Dec
2012 (URL: http://tuberculist.epfl.ch) (email:
tuberculist@epfl.ch).
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis H37Rv"
/strain="H37Rv"
/mol_type="genomic DNA"
/db_xref="taxon:83332"
protein /transl_table=11
/gene="mycP4"
/locus_tag="Rv3449"
/note="Rv3449, (MTCY13E12.02), len: 455 aa. Probable
mycP4,membrane-anchored serine protease (mycosin) (see
citation below), similar to hypothetical unknowns or
proteases from Mycobacterium tuberculosis strains H37Rv
and CDC1551 e.g. AAK48366|MT3998 subtilase family protein
from Mycobacterium tuberculosis strain CDC1551 (411 aa),
FASTA scores: opt: 747, E(): 3.5e-33, (45.65% identity in
416 aa overlap); O05461|Rv3883c|MTCY15F10.29
membrane-anchored mycosin MYCP1 (446 aa), FASTA scores:
opt: 747, E(): 3.8e-33, (45.45% identity in 451 aa
overlap); O53695|Rv0291|MTV035.19 probable
membrane-anchored mycosin MYCP2 (461 aa), FASTA scores:
opt: 660, E(): 1.9e-28, (44.0% identity in 457 aa
overlap); etc. And similar to hypothetical proteases from
Mycobacterium leprae e.g. O33076|MLCB628.04|ML0041
hypothetical 45.7 KDA protein (probable secreted protease)
(446 aa), FASTA scores: opt: 683, E(): 1.1e-29, (43.8%
identity in 450 aa overlap); Q9CD36|ML2528 putative
protease (475 aa), FASTA scores: opt: 608, E():
1.3e-25,(43.0% identity in 451 aa overlap); Q9CBV3|ML1538
possible protease (567 aa), FASTA scores: opt: 389, E():
9.7e-14,(33.8% identity in 562 aa overlap); etc. Also some
similarity to other proteases from several organisms e.g.
O31788|APRX alkaline serine protease from Bacillus
subtilis (442 aa), FASTA scores: opt: 296, E(): 8.3e-09,
(29.4% identity in 313 aa overlap); O86650|SC3C3.17c
putative secreted serine protease from Streptomyces
coelicolor (450 aa), FASTA scores: opt: 279, E(): 7e-08,
(33.55% identity in 343 aa overlap); Q9KBJ7|APRX|BH193
intracellular alkaline serine protease from Bacillus
halodurans (444 aa),FASTA scores: opt: 257, E(): 1.1e-06,
(28.65% identity in 335 aa overlap); O86642|SC3C3.08
serine protease from Streptomyces coelicolor (413 aa),
FASTA scores: opt: 243,E(): 5.7e-06, (38.25% identity in
387 aa overlap); etc. Has putative signal peptide at
N-terminus and hydrophobic stretch at C-terminus. Contains
three signatures typical of subtilase family: aspartic
acid active site (PS00136),histidine active site
(PS00137), and serine active site (PS00138). Belongs to
peptidase family S8 (also known as the subtilase family),
pyrolysin subfamily."
/db_xref="EnsemblGenomes-Gn:Rv3449"
/db_xref="EnsemblGenomes-Tr:CCP46271"
/db_xref="GOA:I6YC58"
/db_xref="InterPro:IPR000209"
/db_xref="InterPro:IPR015500"
/db_xref="InterPro:IPR022398"
/db_xref="InterPro:IPR023827"
/db_xref="InterPro:IPR023828"
/db_xref="InterPro:IPR023834"
/db_xref="InterPro:IPR036852"
/db_xref="UniProtKB/Swiss-Prot:I6YC58"
/inference="protein motif:PROSITE:PS00136"
/inference="protein motif:PROSITE:PS00137"
/inference="protein motif:PROSITE:PS00138"
/experiment="EXISTENCE: identified in proteomics study"
BEGIN
1 MTTSRTLRLL VVSALATLSG LGTPVAHAVS PPPIDERWLP ESALPAPPRP TVQREVCTEV
61 TAESGRAFGR AERSAQLADL DQVWRLTRGA GQRVAVIDTG VARHRRLPKV VAGGDYVFTG
121 DGTADCDAHG TLVAGIIAAA PDAQSDNFSG VAPDVTLISI RQSSSKFAPV GDPSSTGVGD
181 VDTMAKAVRT AADLGASVIN ISSIACVPAA AAPDDRALGA ALAYAVDVKN AVIVAAAGNT
241 GGAAQCPPQA PGVTRDSVTV AVSPAWYDDY VLTVGSVNAQ GEPSAFTLAG PWVDVAATGE
301 AVTSLSPFGD GTVNRLGGQH GSIPISGTSY AAPVVSGLAA LIRARFPTLT ARQVMQRIES
361 TAHHPPAGWD PLVGNGTVDA LAAVSSDSIP QAGTATSDPA PVAVPVPRRS TPGPSDRRAL
421 HTAFAGAAIC LLALMATLAT ASRRLRPGRN GIAGD
//