LOCUS CCP46187.1 154 aa PRT BCT 27-FEB-2015 DEFINITION Mycobacterium tuberculosis H37Rv Probable tRNA/rRNA methylase SpoU (tRNA/rRNA methyltransferase) protein. ACCESSION AL123456-3465 PROTEIN_ID CCP46187.1 SOURCE Mycobacterium tuberculosis H37Rv ORGANISM Mycobacterium tuberculosis H37Rv Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III., Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J., Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. TITLE Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence JOURNAL Nature 393(6685), 537-544(1998). PUBMED 9634230 REMARK Erratum:[Nature 1998 Nov 12;396(6707):190] REFERENCE 2 AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T. TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002). PUBMED 12368430 REFERENCE 3 AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T. TITLE TubercuList--10 years after JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011). PUBMED 20980199 REFERENCE 4 (bases 1 to 4411529) AUTHORS Parkhill J. JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the Mycobacterium tuberculosis sequencing and mapping teams, Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail: parkhill@sanger.ac.uk REFERENCE 5 (bases 1 to 4411532) AUTHORS Lew J.M. JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva 4, SWITZERLAND COMMENT On or before Feb 1, 2013 this sequence version replaced gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250, gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756, gi:38490319, gi:41352785, gi:38490370, gi:41353971. Note: This annotation is from the TubercuList website, Release 26, Dec 2012 (URL: http://tuberculist.epfl.ch) (email: tuberculist@epfl.ch). FEATURES Qualifiers source /organism="Mycobacterium tuberculosis H37Rv" /strain="H37Rv" /mol_type="genomic DNA" /db_xref="taxon:83332" protein /transl_table=11 /gene="spoU" /locus_tag="Rv3366" /note="Rv3366, (MTV004.23), len: 154 aa. Probable spoU,tRNA/rRNA methylase, equivalent to Q9CCU7|ML0419 putative tRNA/rRNA methyltransferase from Mycobacterium leprae (158 aa), FASTA scores: opt: 861, E(): 1.2e-50, (83.75% identity in 154 aa overlap); and O07698|MLCL383.24c rRNA methylase from Mycobacterium leprae (169 aa), FASTA scores: opt: 861,E(): 1.3e-50, (83.75% identity in 154 aa overlap). Also highly similar to many members of the spoU family of rRNA methylases e.g. Q9K199|NMB0268 RNA methyltransferase (TRMH family) from Neisseria meningitidis (serogroup B) (154 aa),FASTA scores: opt: 534, E(): 7.6e-29, (50.0% identity in 154 aa overlap); and Q9JSM8|NMA2218 from Neisseria meningitidis (serogroup A) (154 aa), FASTA scores: opt: 526, E(): 2.6e-28, (49.35% identity in 154 aa overlap); Q9HU57|PA5127 from Pseudomonas aeruginosa (153 aa), FASTA scores: opt: 531, E(): 1.2e-28, (52.95% identity in 151 aa overlap); P33899|YIBK_ECOLI|B3606 from Escherichia coli strain K12 (157 aa), FASTA scores: opt: 511, E(): 2.6e-27,(49.35% identity in 154 aa overlap); etc. Belongs to the RNA methyltransferase TrmH family." /db_xref="EnsemblGenomes-Gn:Rv3366" /db_xref="EnsemblGenomes-Tr:CCP46187" /db_xref="GOA:O50394" /db_xref="InterPro:IPR001537" /db_xref="InterPro:IPR016914" /db_xref="InterPro:IPR029026" /db_xref="InterPro:IPR029028" /db_xref="UniProtKB/TrEMBL:O50394" /experiment="EXISTENCE: identified in proteomics study" BEGIN 1 MFRLLFVSPR IAPNTGNAIR TCAATGCELH LVEPLGFDLS EPKLRRAGLD YHDLASVTVH 61 ASLAHAWEAL SPARVFAFTA QATTLFTNVG YRAGDVLMFG PEPTGLDEAT LADTHITGQV 121 RIPMLAGRRS LNLSNAAAVA VYEAWRQHGF AGAV //