LOCUS CCP45915.1 378 aa PRT BCT 27-FEB-2015 DEFINITION Mycobacterium tuberculosis H37Rv Probable peptide chain release factor 2 PrfB (RF-2) protein. ACCESSION AL123456-3193 PROTEIN_ID CCP45915.1 SOURCE Mycobacterium tuberculosis H37Rv ORGANISM Mycobacterium tuberculosis H37Rv Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III., Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J., Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. TITLE Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence JOURNAL Nature 393(6685), 537-544(1998). PUBMED 9634230 REMARK Erratum:[Nature 1998 Nov 12;396(6707):190] REFERENCE 2 AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T. TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002). PUBMED 12368430 REFERENCE 3 AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T. TITLE TubercuList--10 years after JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011). PUBMED 20980199 REFERENCE 4 (bases 1 to 4411529) AUTHORS Parkhill J. JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the Mycobacterium tuberculosis sequencing and mapping teams, Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail: parkhill@sanger.ac.uk REFERENCE 5 (bases 1 to 4411532) AUTHORS Lew J.M. JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva 4, SWITZERLAND COMMENT On or before Feb 1, 2013 this sequence version replaced gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250, gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756, gi:38490319, gi:41352785, gi:38490370, gi:41353971. Note: This annotation is from the TubercuList website, Release 26, Dec 2012 (URL: http://tuberculist.epfl.ch) (email: tuberculist@epfl.ch). FEATURES Qualifiers source /organism="Mycobacterium tuberculosis H37Rv" /strain="H37Rv" /mol_type="genomic DNA" /db_xref="taxon:83332" protein /transl_table=11 /gene="prfB" /locus_tag="Rv3105c" /note="Rv3105c, (MTCY164.15c), len: 378 aa. Probable prfB,peptide chain release factor 2, equivalent to O32885|RF2_MYCLE|ML0667|MLCB1779.24c from Mycobacterium leprae, FASTA scores: opt: 2197, E(): 1.8e-126, (90.05% identity in 372 aa overlap); and also similar to other peptide chain release factors e.g. Q9L1S3|PRFB from Streptomyces coelicolor (368 aa), FASTA scores: opt: 1674,E(): 1.2e-94, (69.3% identity in 365 aa overlap); O67695|RF2_AQUAE|PRFB|AQ_1840 from Aquifex aeolicus (373 aa), FASTA scores: opt: 1082, E(): 1.3e-58, (44.45% identity in 369 aa overlap); P28367|RF2_BACSU from B. subtilis (366 aa), FASTA scores: opt: 1030, E(): 1.9e-55,(44.0% identity in 359 aa overlap); etc. Also related to Q10605|MTCY373.19|RF1_MYCTU|Rv1299|MT1338 peptide chain release factor 1 (rf-1) (357 aa), FASTA scores: opt: 646,E(): 1.1e-34, (38.6% identity in 350 aa overlap). Contains prokaryotic-type class I peptide chain release factors signature (PS00745). Belongs to the prokaryotic and mitochondrial release factors family." /db_xref="EnsemblGenomes-Gn:Rv3105c" /db_xref="EnsemblGenomes-Tr:CCP45915" /db_xref="GOA:P9WHG1" /db_xref="InterPro:IPR000352" /db_xref="InterPro:IPR004374" /db_xref="InterPro:IPR005139" /db_xref="UniProtKB/Swiss-Prot:P9WHG1" /inference="protein motif:PROSITE:PS00745" /experiment="EXISTENCE: identified in proteomics study" BEGIN 1 MPVTLAAVDP DRQADIAALD CTLTTVERVL DVEGLRSRIE KLEHEASDPH LWDDQTRAQR 61 VTSELSHTQG ELRRVEELRR RLDDLPVLYE LAAEEAGAAA ADAVAEADAE LKSLRADIEA 121 TEVRTLLSGE YDEREALVTI RSGAGGVDAA DWAEMLMRMY IRWAEQHKYP VEVFDTSYAE 181 EAGIKSATFA VHAPFAYGTL SVEQGTHRLV RISPFDNQSR RQTSFAEVEV LPVVETTDHI 241 DIPEGDVRVD VYRSSGPGGQ SVNTTDSAVR LTHIPSGIVV TCQNEKSQLQ NKIAAMRVLQ 301 AKLLERKRLE ERAELDALKA DGGSSWGNQM RSYVLHPYQM VKDLRTEYEV GNPAAVLDGD 361 LDGFLEAGIR WRNRRNDD //