LOCUS CCP45648.1 405 aa PRT BCT 27-FEB-2015
DEFINITION Mycobacterium tuberculosis H37Rv Possible multifunctional
enzyme siroheme synthase CysG: uroporphyrin-III C-methyltransferase
(urogen III methylase) (SUMT) (uroporphyrinogen III methylase)
(UROM) + precorrin-2 oxidase + ferrochelatase protein.
ACCESSION AL123456-2926
PROTEIN_ID CCP45648.1
SOURCE Mycobacterium tuberculosis H37Rv
ORGANISM Mycobacterium tuberculosis H37Rv
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.,
Harris D., Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III.,
Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
Connor R., Davies R., Devlin K., Feltwell T., Gentles S.,
Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J.,
Moule S., Murphy L., Oliver K., Osborne J., Quail M.A.,
Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J.,
Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S.,
Barrell B.G.
TITLE Deciphering the biology of Mycobacterium tuberculosis from the
complete genome sequence
JOURNAL Nature 393(6685), 537-544(1998).
PUBMED 9634230
REMARK Erratum:[Nature 1998 Nov 12;396(6707):190]
REFERENCE 2
AUTHORS Camus J.C., Pryor M.J., Medigue C., Cole S.T.
TITLE Re-annotation of the genome sequence of Mycobacterium tuberculosis
H37Rv
JOURNAL Microbiology (Reading, Engl.) 148(Pt 10), 2967-2973(2002).
PUBMED 12368430
REFERENCE 3
AUTHORS Lew J.M., Kapopoulou A., Jones L.M., Cole S.T.
TITLE TubercuList--10 years after
JOURNAL Tuberculosis (Edinb) 91(1), 1-7(2011).
PUBMED 20980199
REFERENCE 4 (bases 1 to 4411529)
AUTHORS Parkhill J.
JOURNAL Submitted (11-JUN-1998) to the INSDC. Submitted on behalf of the
Mycobacterium tuberculosis sequencing and mapping teams, Sanger
Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA
Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28
rue du Docteur Roux, 75724 Paris Cedex 15, France E-mail:
parkhill@sanger.ac.uk
REFERENCE 5 (bases 1 to 4411532)
AUTHORS Lew J.M.
JOURNAL Submitted (18-DEC-2012) to the INSDC. Lew J., Ecole Polytechnique
Federale de Lausanne, CH-1015, Lausanne, Switzerland, and the Swiss
Institute of Bioinformatics, CMU - Rue Michel-Servet 1, 1211 Geneva
4, SWITZERLAND
COMMENT On or before Feb 1, 2013 this sequence version replaced
gi:41352722, gi:38490165, gi:38490207, gi:41353619, gi:38490250,
gi:38684030, gi:38490288, gi:41353667, gi:41353422, gi:41352756,
gi:38490319, gi:41352785, gi:38490370, gi:41353971.
Note:
This annotation is from the TubercuList website, Release 26, Dec
2012 (URL: http://tuberculist.epfl.ch) (email:
tuberculist@epfl.ch).
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis H37Rv"
/strain="H37Rv"
/mol_type="genomic DNA"
/db_xref="taxon:83332"
protein /transl_table=11
/gene="cysG"
/gene_synonym="cysG2"
/locus_tag="Rv2847c"
/note="Rv2847c, (MTCY24A1.10), len: 405 aa. Possible
cysG,multifunctional enzyme, siroheme synthase containing
uroporphyrin-III c-methyltransferase, precorrin-2 oxidase
and ferrochelatase. C-terminus highly similar to many
uroporphyrin-III c-methyltransferases e.g. Q51720|COBA
uroporphyrinogen III methyltransferase from
Propionibacterium freudenreichii (257 aa), FASTA scores:
opt: 776, E(): 1.5e-39, (48.95% identity in 243 aa
overlap); Q9HMY4|UROM|VNG2331G
S-adenosyl-L-methionine:uroporphyrinogen III
methyltransferase from Halobacterium sp. strain NRC-1 (246
aa), FASTA scores: opt: 704, E(): 3.1e-35, (49.4% identity
in 245 aa overlap); P42437|NASF_BACSU|NASBE
uroporphyrin-III C-methyltransferase from Bacillus
subtilis (483 aa), FASTA scores: opt: 610, E(): 2.4e-29,
(42.1% identity in 240 aa overlap); etc. And highly
similar over entire length to other proteins e.g.
Q9L1C9|SCL11.09c uroporphyrinogen III methyltransferase
from Streptomyces coelicolor (410 aa), FASTA scores: opt:
1481, E(): 5.6e-82,(58.45% identity in 409 aa overlap);
Q9I0M7|CYSG|PA2611 siroheme synthase from Pseudomonas
aeruginosa (465 aa),FASTA scores: opt: 609, E(): 2.7e-29,
(34.7% identity in 444 aa overlap);
P11098|CYSG_ECOLI|B3368|Z4729|ECS4219 siroheme synthase
from Escherichia coli stains O157:H7 and K12 (457 aa),
FASTA scores: opt: 543, E(): 9.1e-27, (31.3% identity in
450 aa overlap); etc. Belongs to a family that groups
SUMT, CYSG, CBIF/COBM and CBIL/COBI. Note that previously
known as cysG2."
/db_xref="EnsemblGenomes-Gn:Rv2847c"
/db_xref="EnsemblGenomes-Tr:CCP45648"
/db_xref="GOA:I6X5I7"
/db_xref="InterPro:IPR000878"
/db_xref="InterPro:IPR006366"
/db_xref="InterPro:IPR012409"
/db_xref="InterPro:IPR014776"
/db_xref="InterPro:IPR014777"
/db_xref="InterPro:IPR035996"
/db_xref="InterPro:IPR036291"
/db_xref="UniProtKB/TrEMBL:I6X5I7"
/experiment="EXISTENCE: identified in proteomics study"
BEGIN
1 MTENPYLVGL RLAGKKVVVV GGGTVAQRRL PLLIASGADV HVIAPSVTPA VEAMDQITLS
61 VRDYRDGDLD GAWYAIAATD DARVNVAVVA EAERRRIFCV RADIAVEGTA VTPASFSYAG
121 LSVGVLAGGE HRRSAAIRSA IREALQQGVI TAQSSDVLSG GVALVGGGPG DPELITVRGR
181 RLLAQADVVV ADRLAPPELL AELPPHVEVI DAAKIPYGRA MAQDAINAVL IERARSGNFV
241 VRLKGGDPFV FARGYEEVLA CAHAGIPVTV VPGVTSAIAV PAMAGVPVTH RAMTHEFVVV
301 SGHLAPGHPE SLVNWDALAA LTGTIVLLMA VERIELFVDV LLKGGRTADT PVLVVQHGTT
361 AAQQTLRATL ADTPEKVRAA GIRPPAIIVI GAVVGLSGVR GLNNS
//